F3MB(PANDER) decreases mice hepatic triglyceride and is associated with decreased DGAT1 expression.
<h4>Objective</h4>Pancreatic-derived factor (PANDER, also named as FAM3B) is secreted by pancreatic α and β cells. Increasing evidence suggests that it may serve a hormonal function related to glycemic and lipid metabolism. In this study, we investigated the effects of PANDER overexpress...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0117156 |
| _version_ | 1818729744280059904 |
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| author | Xiaoqing Mo Chijiao Yang Xuelan Wang Brant R Burkhardt Yangbin Li Haipeng Xia Xiaopei Cao |
| author_facet | Xiaoqing Mo Chijiao Yang Xuelan Wang Brant R Burkhardt Yangbin Li Haipeng Xia Xiaopei Cao |
| author_sort | Xiaoqing Mo |
| collection | DOAJ |
| description | <h4>Objective</h4>Pancreatic-derived factor (PANDER, also named as FAM3B) is secreted by pancreatic α and β cells. Increasing evidence suggests that it may serve a hormonal function related to glycemic and lipid metabolism. In this study, we investigated the effects of PANDER overexpression on hepatic and adipose triglyceride metabolism in high-fat diet-fed male C57BL/6 mice.<h4>Methods</h4>PANDER overexpression was achieved by tail-vein injection of recombinant Ad-PANDER and Ad-GFP injected mice served as a control. The TG metabolism in both groups were compared.<h4>Results</h4>Adenoviral-mediated overexpression of PANDER did not affect body weight, food consumption, or liver enzymes. The triglyceride (TG) content of both liver and adipose tissue was significantly decreased in Ad-PANDER mice (liver: 6.16±1.89 mg/g vs. control 14.95±2.27 mg/g, P<0.05; adipose: 39.31±1.99 mg/100mg vs. 47.22±2.21 mg/100mg, P<0.05). The free fatty acid (FFA) content of adipose tissue in Ad-PANDER mice was also decreased (1.38±0.18 mg/g vs. 2.77±0.31 mg/g, P<0.01). The investigation of key enzymes of triglyceride hydrolysis and FFA oxidation in liver and adipose tissue showed that p-HSL/HSL was significantly increased and that DGAT1 gene and protein expression were significantly reduced in the liver of PANDER-overexpressing mice. PKA phosphorylation was also significantly increased in the livers of Ad-PANDER mice. No differences in ATGL, CPT1, ACOX1, or DGAT2 expression were observed.<h4>Conclusion</h4>Overexpression of PANDER is associated with observable decreases in TG, increases in PKA phosphorylation, and decreased DGAT1 expression, suggesting a possible interrelationship. The mechanisms by which this occurs remain to be elucidated. |
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| format | Article |
| id | doaj.art-b4cda61d71894dde890ae2d2ace79c15 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T22:50:45Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b4cda61d71894dde890ae2d2ace79c152022-12-21T21:29:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01102e011715610.1371/journal.pone.0117156F3MB(PANDER) decreases mice hepatic triglyceride and is associated with decreased DGAT1 expression.Xiaoqing MoChijiao YangXuelan WangBrant R BurkhardtYangbin LiHaipeng XiaXiaopei Cao<h4>Objective</h4>Pancreatic-derived factor (PANDER, also named as FAM3B) is secreted by pancreatic α and β cells. Increasing evidence suggests that it may serve a hormonal function related to glycemic and lipid metabolism. In this study, we investigated the effects of PANDER overexpression on hepatic and adipose triglyceride metabolism in high-fat diet-fed male C57BL/6 mice.<h4>Methods</h4>PANDER overexpression was achieved by tail-vein injection of recombinant Ad-PANDER and Ad-GFP injected mice served as a control. The TG metabolism in both groups were compared.<h4>Results</h4>Adenoviral-mediated overexpression of PANDER did not affect body weight, food consumption, or liver enzymes. The triglyceride (TG) content of both liver and adipose tissue was significantly decreased in Ad-PANDER mice (liver: 6.16±1.89 mg/g vs. control 14.95±2.27 mg/g, P<0.05; adipose: 39.31±1.99 mg/100mg vs. 47.22±2.21 mg/100mg, P<0.05). The free fatty acid (FFA) content of adipose tissue in Ad-PANDER mice was also decreased (1.38±0.18 mg/g vs. 2.77±0.31 mg/g, P<0.01). The investigation of key enzymes of triglyceride hydrolysis and FFA oxidation in liver and adipose tissue showed that p-HSL/HSL was significantly increased and that DGAT1 gene and protein expression were significantly reduced in the liver of PANDER-overexpressing mice. PKA phosphorylation was also significantly increased in the livers of Ad-PANDER mice. No differences in ATGL, CPT1, ACOX1, or DGAT2 expression were observed.<h4>Conclusion</h4>Overexpression of PANDER is associated with observable decreases in TG, increases in PKA phosphorylation, and decreased DGAT1 expression, suggesting a possible interrelationship. The mechanisms by which this occurs remain to be elucidated.https://doi.org/10.1371/journal.pone.0117156 |
| spellingShingle | Xiaoqing Mo Chijiao Yang Xuelan Wang Brant R Burkhardt Yangbin Li Haipeng Xia Xiaopei Cao F3MB(PANDER) decreases mice hepatic triglyceride and is associated with decreased DGAT1 expression. PLoS ONE |
| title | F3MB(PANDER) decreases mice hepatic triglyceride and is associated with decreased DGAT1 expression. |
| title_full | F3MB(PANDER) decreases mice hepatic triglyceride and is associated with decreased DGAT1 expression. |
| title_fullStr | F3MB(PANDER) decreases mice hepatic triglyceride and is associated with decreased DGAT1 expression. |
| title_full_unstemmed | F3MB(PANDER) decreases mice hepatic triglyceride and is associated with decreased DGAT1 expression. |
| title_short | F3MB(PANDER) decreases mice hepatic triglyceride and is associated with decreased DGAT1 expression. |
| title_sort | f3mb pander decreases mice hepatic triglyceride and is associated with decreased dgat1 expression |
| url | https://doi.org/10.1371/journal.pone.0117156 |
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