Performance of Two Matrix-Assisted Laser Desorption Ionization–Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Systems for Identification of the Viridans Group Streptococci
Fen Pan,1,2,* Na Zhao,3,* Wantong Zhao,1 Chun Wang,1 Yan Sun,1 Haomin Zhang,3 Juanxiu Qin,3 Qian Liu,3 Hong Zhang1,2 1Department of Clinical Laboratory, Shanghai Children’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of China; 2Institute o...
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| Format: | Article |
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Dove Medical Press
2023-05-01
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| Series: | Infection and Drug Resistance |
| Subjects: | |
| Online Access: | https://www.dovepress.com/performance-of-two-matrix-assisted-laser-desorption-ionizationtime-of--peer-reviewed-fulltext-article-IDR |
| _version_ | 1797829228446613504 |
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| author | Pan F Zhao N Zhao W Wang C Sun Y Zhang H Qin J Liu Q Zhang H |
| author_facet | Pan F Zhao N Zhao W Wang C Sun Y Zhang H Qin J Liu Q Zhang H |
| author_sort | Pan F |
| collection | DOAJ |
| description | Fen Pan,1,2,* Na Zhao,3,* Wantong Zhao,1 Chun Wang,1 Yan Sun,1 Haomin Zhang,3 Juanxiu Qin,3 Qian Liu,3 Hong Zhang1,2 1Department of Clinical Laboratory, Shanghai Children’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of China; 2Institute of Pediatric Infection, Immunity, and Critical Care Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai, 200062, People’s Republic of China; 3Department of Laboratory Medicine, RenJi Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hong Zhang, Department of Clinical Laboratory, Shanghai Children’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of China, Tel +86 189 1712 8200, Email schjyk2015@126.com Qian Liu, Department of Laboratory Medicine, RenJi Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, People’s Republic of China, Email qq2005011@163.comBackground: Due to similar colony morphology among viridans group streptococci (VGS), the differentiation of VGS species remains difficult in routine clinical microbiology. Recently, matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS) has been described as a fast method for identifying various bacteria at species level, and also for the VGS strains.Methods: A total of 277 VGS isolates were identified with the two MALDI-TOF MS systems (VITEK MS and Bruker Biotyper). The tuf and rpoB gene sequencing was used as the reference identification method for comparison.Results: Based on tuf and rpoB gene sequencing, 84 isolates were S. pneumoniae and 193 strains were other VGS isolates including S. anginosus group (n=91, 47.2%), S. mitis group (n=80, 41.5%), S. bovis group (n=11, 5.7%), S. salivarius group (n=10, 5.2%), and S. mutans group (n=1, 0.5%). VITEK MS and Bruker Biotyper accurately identified 94.6% and 89.9% of all VGS isolates, respectively. VITEK MS showed better identification results than Bruker Biotyper for S. mitis group including S. pneumoniae and S. bovis group, but for other VGS isolates, two MALDI-TOF MS systems showed comparable identification performance. However, VITEK MS was able to identify S. gallolyticus to the subspecies level with high-confidence (S. gallolyticus ssp. pasteurianus), while the Bruker Biotyper system could not. While Bruker Biotyper system could be able to correctly differentiate the subspecies of S. salivarius from S. vestibularis, VITEK MS poorly identify.Conclusion: This study demonstrated that two MALDI-TOF MS systems allowed discrimination for most VGS isolates with different identification performance, but Bruker Biotyper could produce more misidentifications and VITEK MS system. It is crucial to be familiar with the performance of MALDI-TOF MS systems used in clinical microbiology.Keywords: MALDI-TOF MS, viridans group streptococci, identification, Streptococcus pneumoniae |
| first_indexed | 2024-04-09T13:17:01Z |
| format | Article |
| id | doaj.art-b4cf77b63ef6480fa1ea30fd0f5a1396 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-04-09T13:17:01Z |
| publishDate | 2023-05-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-b4cf77b63ef6480fa1ea30fd0f5a13962023-05-11T18:59:32ZengDove Medical PressInfection and Drug Resistance1178-69732023-05-01Volume 162901290983641Performance of Two Matrix-Assisted Laser Desorption Ionization–Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Systems for Identification of the Viridans Group StreptococciPan FZhao NZhao WWang CSun YZhang HQin JLiu QZhang HFen Pan,1,2,* Na Zhao,3,* Wantong Zhao,1 Chun Wang,1 Yan Sun,1 Haomin Zhang,3 Juanxiu Qin,3 Qian Liu,3 Hong Zhang1,2 1Department of Clinical Laboratory, Shanghai Children’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of China; 2Institute of Pediatric Infection, Immunity, and Critical Care Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai, 200062, People’s Republic of China; 3Department of Laboratory Medicine, RenJi Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hong Zhang, Department of Clinical Laboratory, Shanghai Children’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of China, Tel +86 189 1712 8200, Email schjyk2015@126.com Qian Liu, Department of Laboratory Medicine, RenJi Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, People’s Republic of China, Email qq2005011@163.comBackground: Due to similar colony morphology among viridans group streptococci (VGS), the differentiation of VGS species remains difficult in routine clinical microbiology. Recently, matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS) has been described as a fast method for identifying various bacteria at species level, and also for the VGS strains.Methods: A total of 277 VGS isolates were identified with the two MALDI-TOF MS systems (VITEK MS and Bruker Biotyper). The tuf and rpoB gene sequencing was used as the reference identification method for comparison.Results: Based on tuf and rpoB gene sequencing, 84 isolates were S. pneumoniae and 193 strains were other VGS isolates including S. anginosus group (n=91, 47.2%), S. mitis group (n=80, 41.5%), S. bovis group (n=11, 5.7%), S. salivarius group (n=10, 5.2%), and S. mutans group (n=1, 0.5%). VITEK MS and Bruker Biotyper accurately identified 94.6% and 89.9% of all VGS isolates, respectively. VITEK MS showed better identification results than Bruker Biotyper for S. mitis group including S. pneumoniae and S. bovis group, but for other VGS isolates, two MALDI-TOF MS systems showed comparable identification performance. However, VITEK MS was able to identify S. gallolyticus to the subspecies level with high-confidence (S. gallolyticus ssp. pasteurianus), while the Bruker Biotyper system could not. While Bruker Biotyper system could be able to correctly differentiate the subspecies of S. salivarius from S. vestibularis, VITEK MS poorly identify.Conclusion: This study demonstrated that two MALDI-TOF MS systems allowed discrimination for most VGS isolates with different identification performance, but Bruker Biotyper could produce more misidentifications and VITEK MS system. It is crucial to be familiar with the performance of MALDI-TOF MS systems used in clinical microbiology.Keywords: MALDI-TOF MS, viridans group streptococci, identification, Streptococcus pneumoniaehttps://www.dovepress.com/performance-of-two-matrix-assisted-laser-desorption-ionizationtime-of--peer-reviewed-fulltext-article-IDRmaldi-tof msviridans group streptococciidentificationstreptococcus pneumoniae |
| spellingShingle | Pan F Zhao N Zhao W Wang C Sun Y Zhang H Qin J Liu Q Zhang H Performance of Two Matrix-Assisted Laser Desorption Ionization–Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Systems for Identification of the Viridans Group Streptococci Infection and Drug Resistance maldi-tof ms viridans group streptococci identification streptococcus pneumoniae |
| title | Performance of Two Matrix-Assisted Laser Desorption Ionization–Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Systems for Identification of the Viridans Group Streptococci |
| title_full | Performance of Two Matrix-Assisted Laser Desorption Ionization–Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Systems for Identification of the Viridans Group Streptococci |
| title_fullStr | Performance of Two Matrix-Assisted Laser Desorption Ionization–Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Systems for Identification of the Viridans Group Streptococci |
| title_full_unstemmed | Performance of Two Matrix-Assisted Laser Desorption Ionization–Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Systems for Identification of the Viridans Group Streptococci |
| title_short | Performance of Two Matrix-Assisted Laser Desorption Ionization–Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Systems for Identification of the Viridans Group Streptococci |
| title_sort | performance of two matrix assisted laser desorption ionization ndash time of flight mass spectrometry maldi tof ms systems for identification of the viridans group streptococci |
| topic | maldi-tof ms viridans group streptococci identification streptococcus pneumoniae |
| url | https://www.dovepress.com/performance-of-two-matrix-assisted-laser-desorption-ionizationtime-of--peer-reviewed-fulltext-article-IDR |
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