Identification of three Iranian species of the genus <em>Rattus</em> (Rodentia, Muridae) using a PCR-RFLP technique on mitochondrial DNA
<strong>Abstract</strong> Three species of the genus <em>Rattus</em> Fisher, 1803 have been reported from Iran: the brown rat (<em>R. norvegicus</em>), the black rat (<em>R. rattus</em&a...
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| Format: | Article |
| Language: | English |
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Associazione Teriologica Italiana
2009-11-01
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| Series: | Hystrix, the Italian Journal of Mammalogy |
| Subjects: | |
| Online Access: | http://www.italian-journal-of-mammalogy.it/article/view/4435 |
| _version_ | 1828369415408713728 |
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| author | safieh Akbary rad Razieh Jalal Jamshid Darvish Mayam Moghadam Matin |
| author_facet | safieh Akbary rad Razieh Jalal Jamshid Darvish Mayam Moghadam Matin |
| author_sort | safieh Akbary rad |
| collection | DOAJ |
| description | <strong>Abstract</strong> Three species of the genus <em>Rattus</em> Fisher, 1803 have been reported from Iran: the brown rat (<em>R. norvegicus</em>), the black rat (<em>R. rattus</em>) and the Himalayan rat (<em>R. pyctoris</em>). The first two were introduced, whilst <em>R. pyctoris</em> is native and lives in mountainous regions from Pakistan to north-eastern Iran. In this study, the mitochondrial DNA from twenty six rats were analysed using a PCR-RFLP (Polymerase Chain Reaction - Restriction Fragments Length Polymorphism) method to investigate inter-specific variation. Part of the 16S rRNA and cytochrome b genes were amplified and digested with three restriction enzymes: AluI, MboI and HinfI. Restriction fragments resulted in four different haplotypes and allowed to distinguish the three <em>Rattus</em> species. Our results suggest that the Himalayan rats are more closely related to <em>R. rattus</em> than to <em>R. norvegicus</em> and provide the basics for further phylogenetic studies. <strong>Riassunto</strong> <strong>Identificazione di tre specie iraniane del genere <em>Rattus</em> (Rodentia, Muridae) tramite PCR-RFLP su DNA mitocondriale.</strong> Tre specie del genere <em>Rattus</em> risultano diffuse in Iran: il surmolotto (<em>R. norvegicus</em>), il ratto nero (<em>R. rattus</em>) e il ratto himalayano (<em>R. pyctoris</em>). Le prime due specie sono state introdotte, mentre <em>R. pyctoris</em> è presente nelle aree montane che si sviluppano dal Pakistan all’Iran nordorientale. In questo studio, il DNA mitocondriale di 26 individui è stato analizzato tramite PCR-RFLP per evidenziare variazioni inter-specifiche. Parte dei geni del rRNA 16S e del citocromo b è stata amplificata e quindi sottoposta a digestione tramite tre diversi enzimi: AluI, MboI e HinfI. I frammenti di restrizione hanno permesso di individuare quattro aplotipi mitocondriali e di distinguere le tre specie. I risultati ottenuti suggeriscono che il ratto himalayano sia più vicino a <em>R. rattus</em> che non a R. norvegicus e pongono le basi per ulteriori studi filogenetici. |
| first_indexed | 2024-04-14T06:24:34Z |
| format | Article |
| id | doaj.art-b4cff13c78c64bb6966429b850d1b2cd |
| institution | Directory Open Access Journal |
| issn | 0394-1914 1825-5272 |
| language | English |
| last_indexed | 2024-04-14T06:24:34Z |
| publishDate | 2009-11-01 |
| publisher | Associazione Teriologica Italiana |
| record_format | Article |
| series | Hystrix, the Italian Journal of Mammalogy |
| spelling | doaj.art-b4cff13c78c64bb6966429b850d1b2cd2022-12-22T02:07:54ZengAssociazione Teriologica ItalianaHystrix, the Italian Journal of Mammalogy0394-19141825-52722009-11-0120110.4404/hystrix-20.1-4435Identification of three Iranian species of the genus <em>Rattus</em> (Rodentia, Muridae) using a PCR-RFLP technique on mitochondrial DNAsafieh Akbary radRazieh JalalJamshid DarvishMayam Moghadam Matin<strong>Abstract</strong> Three species of the genus <em>Rattus</em> Fisher, 1803 have been reported from Iran: the brown rat (<em>R. norvegicus</em>), the black rat (<em>R. rattus</em>) and the Himalayan rat (<em>R. pyctoris</em>). The first two were introduced, whilst <em>R. pyctoris</em> is native and lives in mountainous regions from Pakistan to north-eastern Iran. In this study, the mitochondrial DNA from twenty six rats were analysed using a PCR-RFLP (Polymerase Chain Reaction - Restriction Fragments Length Polymorphism) method to investigate inter-specific variation. Part of the 16S rRNA and cytochrome b genes were amplified and digested with three restriction enzymes: AluI, MboI and HinfI. Restriction fragments resulted in four different haplotypes and allowed to distinguish the three <em>Rattus</em> species. Our results suggest that the Himalayan rats are more closely related to <em>R. rattus</em> than to <em>R. norvegicus</em> and provide the basics for further phylogenetic studies. <strong>Riassunto</strong> <strong>Identificazione di tre specie iraniane del genere <em>Rattus</em> (Rodentia, Muridae) tramite PCR-RFLP su DNA mitocondriale.</strong> Tre specie del genere <em>Rattus</em> risultano diffuse in Iran: il surmolotto (<em>R. norvegicus</em>), il ratto nero (<em>R. rattus</em>) e il ratto himalayano (<em>R. pyctoris</em>). Le prime due specie sono state introdotte, mentre <em>R. pyctoris</em> è presente nelle aree montane che si sviluppano dal Pakistan all’Iran nordorientale. In questo studio, il DNA mitocondriale di 26 individui è stato analizzato tramite PCR-RFLP per evidenziare variazioni inter-specifiche. Parte dei geni del rRNA 16S e del citocromo b è stata amplificata e quindi sottoposta a digestione tramite tre diversi enzimi: AluI, MboI e HinfI. I frammenti di restrizione hanno permesso di individuare quattro aplotipi mitocondriali e di distinguere le tre specie. I risultati ottenuti suggeriscono che il ratto himalayano sia più vicino a <em>R. rattus</em> che non a R. norvegicus e pongono le basi per ulteriori studi filogenetici.http://www.italian-journal-of-mammalogy.it/article/view/4435, mtDNA, PCR-RFLP, taxonomy |
| spellingShingle | safieh Akbary rad Razieh Jalal Jamshid Darvish Mayam Moghadam Matin Identification of three Iranian species of the genus <em>Rattus</em> (Rodentia, Muridae) using a PCR-RFLP technique on mitochondrial DNA Hystrix, the Italian Journal of Mammalogy , mtDNA, PCR-RFLP, taxonomy |
| title | Identification of three Iranian species of the genus <em>Rattus</em> (Rodentia, Muridae) using a PCR-RFLP technique on mitochondrial DNA |
| title_full | Identification of three Iranian species of the genus <em>Rattus</em> (Rodentia, Muridae) using a PCR-RFLP technique on mitochondrial DNA |
| title_fullStr | Identification of three Iranian species of the genus <em>Rattus</em> (Rodentia, Muridae) using a PCR-RFLP technique on mitochondrial DNA |
| title_full_unstemmed | Identification of three Iranian species of the genus <em>Rattus</em> (Rodentia, Muridae) using a PCR-RFLP technique on mitochondrial DNA |
| title_short | Identification of three Iranian species of the genus <em>Rattus</em> (Rodentia, Muridae) using a PCR-RFLP technique on mitochondrial DNA |
| title_sort | identification of three iranian species of the genus lt em gt rattus lt em gt rodentia muridae using a pcr rflp technique on mitochondrial dna |
| topic | , mtDNA, PCR-RFLP, taxonomy |
| url | http://www.italian-journal-of-mammalogy.it/article/view/4435 |
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