Identification and Characterization of <i>Nectria pseudotrichia</i> Associated with Camellia Canker Disease in China
<i>Camellia japonica</i> is a native tree species with high economic value that is widely cultivated in southern China. In recent years, canker disease has been observed in camellia plantations in Zhejiang Province, China, with the disease incidence rate in some plantations exceeding 20%...
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MDPI AG
2021-12-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/13/1/29 |
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| author | Xiansheng Geng Ying Liu Jiyuan Li Zhihong Li Jinping Shu Guiyang Wu |
| author_facet | Xiansheng Geng Ying Liu Jiyuan Li Zhihong Li Jinping Shu Guiyang Wu |
| author_sort | Xiansheng Geng |
| collection | DOAJ |
| description | <i>Camellia japonica</i> is a native tree species with high economic value that is widely cultivated in southern China. In recent years, canker disease has been observed in camellia plantations in Zhejiang Province, China, with the disease incidence rate in some plantations exceeding 20%. Canker disease severely affects the trunks and branches of <i>C. japonica</i> in China, but the causal agent has not yet been identified. In this study, the pathogen was isolated from infected <i>C. japonica</i> tissues through a conventional tissue isolation approach. Species identification was conducted using morphological methods combined with multilocus phylogenetic analysis. Pathogenicity was tested based on Koch’s postulates. The results showed that the pathogen could be isolated from the diseased bark of <i>C. japonica</i> ‘Hongluzhen’. The pathogen was identified as <i>Nectria pseudotrichia</i> based on morphological, cultural, and molecular traits. The inoculation of the pathogen into <i>C. japonica</i> ‘Hongluzhen’ caused necrotic lesions on healthy seedlings, and the fungus <i>N. pseudotrichia</i> could be re-isolated from such lesions. Therefore, <i>N. pseudotrichia</i> is the causal agent of canker disease affecting <i>C. japonica</i> in China. |
| first_indexed | 2024-03-10T01:28:59Z |
| format | Article |
| id | doaj.art-b4d0ea3642ce461a9cd4fd62230d1737 |
| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-10T01:28:59Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Forests |
| spelling | doaj.art-b4d0ea3642ce461a9cd4fd62230d17372023-11-23T13:46:38ZengMDPI AGForests1999-49072021-12-011312910.3390/f13010029Identification and Characterization of <i>Nectria pseudotrichia</i> Associated with Camellia Canker Disease in ChinaXiansheng Geng0Ying Liu1Jiyuan Li2Zhihong Li3Jinping Shu4Guiyang Wu5Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, ChinaResearch Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, ChinaResearch Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, ChinaResearch Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, ChinaResearch Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, ChinaAgricultural Technology Service Station of Fenghua City, Ningbo 315502, China<i>Camellia japonica</i> is a native tree species with high economic value that is widely cultivated in southern China. In recent years, canker disease has been observed in camellia plantations in Zhejiang Province, China, with the disease incidence rate in some plantations exceeding 20%. Canker disease severely affects the trunks and branches of <i>C. japonica</i> in China, but the causal agent has not yet been identified. In this study, the pathogen was isolated from infected <i>C. japonica</i> tissues through a conventional tissue isolation approach. Species identification was conducted using morphological methods combined with multilocus phylogenetic analysis. Pathogenicity was tested based on Koch’s postulates. The results showed that the pathogen could be isolated from the diseased bark of <i>C. japonica</i> ‘Hongluzhen’. The pathogen was identified as <i>Nectria pseudotrichia</i> based on morphological, cultural, and molecular traits. The inoculation of the pathogen into <i>C. japonica</i> ‘Hongluzhen’ caused necrotic lesions on healthy seedlings, and the fungus <i>N. pseudotrichia</i> could be re-isolated from such lesions. Therefore, <i>N. pseudotrichia</i> is the causal agent of canker disease affecting <i>C. japonica</i> in China.https://www.mdpi.com/1999-4907/13/1/29<i>Camellia japonica</i>canker diseasepathogenKoch’s postulates |
| spellingShingle | Xiansheng Geng Ying Liu Jiyuan Li Zhihong Li Jinping Shu Guiyang Wu Identification and Characterization of <i>Nectria pseudotrichia</i> Associated with Camellia Canker Disease in China Forests <i>Camellia japonica</i> canker disease pathogen Koch’s postulates |
| title | Identification and Characterization of <i>Nectria pseudotrichia</i> Associated with Camellia Canker Disease in China |
| title_full | Identification and Characterization of <i>Nectria pseudotrichia</i> Associated with Camellia Canker Disease in China |
| title_fullStr | Identification and Characterization of <i>Nectria pseudotrichia</i> Associated with Camellia Canker Disease in China |
| title_full_unstemmed | Identification and Characterization of <i>Nectria pseudotrichia</i> Associated with Camellia Canker Disease in China |
| title_short | Identification and Characterization of <i>Nectria pseudotrichia</i> Associated with Camellia Canker Disease in China |
| title_sort | identification and characterization of i nectria pseudotrichia i associated with camellia canker disease in china |
| topic | <i>Camellia japonica</i> canker disease pathogen Koch’s postulates |
| url | https://www.mdpi.com/1999-4907/13/1/29 |
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