Anti-Metastatic Function of Extracellular Vesicles Derived from <i>Nanog</i>-Overexpressing Melanoma
A metastatic melanoma cell line B16-F10 (F10) was modified to a more undifferentiated state by <i>Nanog</i> overexpression. The produced cell line <i>Nanog</i><sup>+</sup>F10 showed a higher metastatic potential than F10. Instead of whole cells, the extracellular...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Current Oncology |
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| Online Access: | https://www.mdpi.com/1718-7729/29/2/88 |
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| author | Tomohiro Hatakenaka Nahoko Matsuki Seiya Minagawa Celine Swee May Khoo Mikako Saito |
| author_facet | Tomohiro Hatakenaka Nahoko Matsuki Seiya Minagawa Celine Swee May Khoo Mikako Saito |
| author_sort | Tomohiro Hatakenaka |
| collection | DOAJ |
| description | A metastatic melanoma cell line B16-F10 (F10) was modified to a more undifferentiated state by <i>Nanog</i> overexpression. The produced cell line <i>Nanog</i><sup>+</sup>F10 showed a higher metastatic potential than F10. Instead of whole cells, the extracellular vesicles (EVs) therefrom were investigated about their possible role as an autovaccine against metastasis. EVs from <i>Nanog</i><sup>+</sup>F10 cells (<i>Nanog</i><sup>+</sup>F10-EVs) could suppress the metastasis, contrasting the EVs from less metastatic F10 cells (F10-EVs) enhanced metastasis. The involvement of TGF-β1 in the role of <i>Nanog</i><sup>+</sup>F10-EVs was analyzed, as TGF-β1 was a secretory cytokine being affected most intensively by <i>Nanog</i> overexpression. It was suggested to be crucial that the TGF-β1 concentration in <i>Nanog</i><sup>+</sup>F10-EVs should be as low as 1.6 pg/μg for its metastasis-suppressive role. In response to <i>Nanog</i><sup>+</sup>F10-EVs, immunoreaction was observed in liver, indicating the specific decrease in the number of tumor-promotive CD163-positive macrophages. These indicate a possibility of <i>Nanog</i><sup>+</sup>F10-EVs as a novel autovaccine candidate against melanoma metastasis. |
| first_indexed | 2024-03-09T22:13:38Z |
| format | Article |
| id | doaj.art-b4d15c03fcfa4773822bbf6eaf5de4e7 |
| institution | Directory Open Access Journal |
| issn | 1198-0052 1718-7729 |
| language | English |
| last_indexed | 2024-03-09T22:13:38Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Oncology |
| spelling | doaj.art-b4d15c03fcfa4773822bbf6eaf5de4e72023-11-23T19:27:15ZengMDPI AGCurrent Oncology1198-00521718-77292022-02-012921029104610.3390/curroncol29020088Anti-Metastatic Function of Extracellular Vesicles Derived from <i>Nanog</i>-Overexpressing MelanomaTomohiro Hatakenaka0Nahoko Matsuki1Seiya Minagawa2Celine Swee May Khoo3Mikako Saito4Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, JapanDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, JapanDepartment of Industrial Technology and Innovation, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, JapanDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, JapanDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, JapanA metastatic melanoma cell line B16-F10 (F10) was modified to a more undifferentiated state by <i>Nanog</i> overexpression. The produced cell line <i>Nanog</i><sup>+</sup>F10 showed a higher metastatic potential than F10. Instead of whole cells, the extracellular vesicles (EVs) therefrom were investigated about their possible role as an autovaccine against metastasis. EVs from <i>Nanog</i><sup>+</sup>F10 cells (<i>Nanog</i><sup>+</sup>F10-EVs) could suppress the metastasis, contrasting the EVs from less metastatic F10 cells (F10-EVs) enhanced metastasis. The involvement of TGF-β1 in the role of <i>Nanog</i><sup>+</sup>F10-EVs was analyzed, as TGF-β1 was a secretory cytokine being affected most intensively by <i>Nanog</i> overexpression. It was suggested to be crucial that the TGF-β1 concentration in <i>Nanog</i><sup>+</sup>F10-EVs should be as low as 1.6 pg/μg for its metastasis-suppressive role. In response to <i>Nanog</i><sup>+</sup>F10-EVs, immunoreaction was observed in liver, indicating the specific decrease in the number of tumor-promotive CD163-positive macrophages. These indicate a possibility of <i>Nanog</i><sup>+</sup>F10-EVs as a novel autovaccine candidate against melanoma metastasis.https://www.mdpi.com/1718-7729/29/2/88melanoma<i>Nanog</i> overexpressionextracellular vesiclesautovaccineTGF-β1 |
| spellingShingle | Tomohiro Hatakenaka Nahoko Matsuki Seiya Minagawa Celine Swee May Khoo Mikako Saito Anti-Metastatic Function of Extracellular Vesicles Derived from <i>Nanog</i>-Overexpressing Melanoma Current Oncology melanoma <i>Nanog</i> overexpression extracellular vesicles autovaccine TGF-β1 |
| title | Anti-Metastatic Function of Extracellular Vesicles Derived from <i>Nanog</i>-Overexpressing Melanoma |
| title_full | Anti-Metastatic Function of Extracellular Vesicles Derived from <i>Nanog</i>-Overexpressing Melanoma |
| title_fullStr | Anti-Metastatic Function of Extracellular Vesicles Derived from <i>Nanog</i>-Overexpressing Melanoma |
| title_full_unstemmed | Anti-Metastatic Function of Extracellular Vesicles Derived from <i>Nanog</i>-Overexpressing Melanoma |
| title_short | Anti-Metastatic Function of Extracellular Vesicles Derived from <i>Nanog</i>-Overexpressing Melanoma |
| title_sort | anti metastatic function of extracellular vesicles derived from i nanog i overexpressing melanoma |
| topic | melanoma <i>Nanog</i> overexpression extracellular vesicles autovaccine TGF-β1 |
| url | https://www.mdpi.com/1718-7729/29/2/88 |
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