Transplantation of insulin-producing cells from umbilical cord mesenchymal stem cells for the treatment of streptozotocin-induced diabetic rats
<p>Abstract</p> <p>Background</p> <p>Although diabetes mellitus (DM) can be treated with islet transplantation, a scarcity of donors limits the utility of this technique. This study investigated whether human mesenchymal stem cells (MSCs) from umbilical cord could be in...
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| Format: | Article |
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BMC
2012-04-01
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| Series: | Journal of Biomedical Science |
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| Online Access: | http://www.jbiomedsci.com/content/19/1/47 |
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| author | Tsai Pei-Jiun Wang Hwai-Shi Shyr Yi-Ming Weng Zen-Chung Tai Ling-Chen Shyu Jia-Fwu Chen Tien-Hua |
| author_facet | Tsai Pei-Jiun Wang Hwai-Shi Shyr Yi-Ming Weng Zen-Chung Tai Ling-Chen Shyu Jia-Fwu Chen Tien-Hua |
| author_sort | Tsai Pei-Jiun |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Although diabetes mellitus (DM) can be treated with islet transplantation, a scarcity of donors limits the utility of this technique. This study investigated whether human mesenchymal stem cells (MSCs) from umbilical cord could be induced efficiently to differentiate into insulin-producing cells. Secondly, we evaluated the effect of portal vein transplantation of these differentiated cells in the treatment of streptozotocin-induced diabetes in rats.</p> <p>Methods</p> <p>MSCs from human umbilical cord were induced in three stages to differentiate into insulin-producing cells and evaluated by immunocytochemistry, reverse transcriptase, and real-time PCR, and ELISA. Differentiated cells were transplanted into the liver of diabetic rats using a Port-A catheter via the portal vein. Blood glucose levels were monitored weekly.</p> <p>Results</p> <p>Human nuclei and C-peptide were detected in the rat liver by immunohistochemistry. Pancreatic β-cell development-related genes were expressed in the differentiated cells. C-peptide release was increased after glucose challenge <it>in vitro</it>. Furthermore, after transplantation of differentiated cells into the diabetic rats, blood sugar level decreased. Insulin-producing cells containing human C-peptide and human nuclei were located in the liver.</p> <p>Conclusion</p> <p>Thus, a Port-A catheter can be used to transplant differentiated insulin-producing cells from human MSCs into the portal vein to alleviate hyperglycemia among diabetic rats.</p> |
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| id | doaj.art-b4d2a10ca1964e899ba63c909d99a3ff |
| institution | Directory Open Access Journal |
| issn | 1021-7770 1423-0127 |
| language | English |
| last_indexed | 2024-04-13T00:37:00Z |
| publishDate | 2012-04-01 |
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| series | Journal of Biomedical Science |
| spelling | doaj.art-b4d2a10ca1964e899ba63c909d99a3ff2022-12-22T03:10:19ZengBMCJournal of Biomedical Science1021-77701423-01272012-04-011914710.1186/1423-0127-19-47Transplantation of insulin-producing cells from umbilical cord mesenchymal stem cells for the treatment of streptozotocin-induced diabetic ratsTsai Pei-JiunWang Hwai-ShiShyr Yi-MingWeng Zen-ChungTai Ling-ChenShyu Jia-FwuChen Tien-Hua<p>Abstract</p> <p>Background</p> <p>Although diabetes mellitus (DM) can be treated with islet transplantation, a scarcity of donors limits the utility of this technique. This study investigated whether human mesenchymal stem cells (MSCs) from umbilical cord could be induced efficiently to differentiate into insulin-producing cells. Secondly, we evaluated the effect of portal vein transplantation of these differentiated cells in the treatment of streptozotocin-induced diabetes in rats.</p> <p>Methods</p> <p>MSCs from human umbilical cord were induced in three stages to differentiate into insulin-producing cells and evaluated by immunocytochemistry, reverse transcriptase, and real-time PCR, and ELISA. Differentiated cells were transplanted into the liver of diabetic rats using a Port-A catheter via the portal vein. Blood glucose levels were monitored weekly.</p> <p>Results</p> <p>Human nuclei and C-peptide were detected in the rat liver by immunohistochemistry. Pancreatic β-cell development-related genes were expressed in the differentiated cells. C-peptide release was increased after glucose challenge <it>in vitro</it>. Furthermore, after transplantation of differentiated cells into the diabetic rats, blood sugar level decreased. Insulin-producing cells containing human C-peptide and human nuclei were located in the liver.</p> <p>Conclusion</p> <p>Thus, a Port-A catheter can be used to transplant differentiated insulin-producing cells from human MSCs into the portal vein to alleviate hyperglycemia among diabetic rats.</p>http://www.jbiomedsci.com/content/19/1/47Mesenchymal stem cellPortal veinInsulin-producing cellsTransplant |
| spellingShingle | Tsai Pei-Jiun Wang Hwai-Shi Shyr Yi-Ming Weng Zen-Chung Tai Ling-Chen Shyu Jia-Fwu Chen Tien-Hua Transplantation of insulin-producing cells from umbilical cord mesenchymal stem cells for the treatment of streptozotocin-induced diabetic rats Journal of Biomedical Science Mesenchymal stem cell Portal vein Insulin-producing cells Transplant |
| title | Transplantation of insulin-producing cells from umbilical cord mesenchymal stem cells for the treatment of streptozotocin-induced diabetic rats |
| title_full | Transplantation of insulin-producing cells from umbilical cord mesenchymal stem cells for the treatment of streptozotocin-induced diabetic rats |
| title_fullStr | Transplantation of insulin-producing cells from umbilical cord mesenchymal stem cells for the treatment of streptozotocin-induced diabetic rats |
| title_full_unstemmed | Transplantation of insulin-producing cells from umbilical cord mesenchymal stem cells for the treatment of streptozotocin-induced diabetic rats |
| title_short | Transplantation of insulin-producing cells from umbilical cord mesenchymal stem cells for the treatment of streptozotocin-induced diabetic rats |
| title_sort | transplantation of insulin producing cells from umbilical cord mesenchymal stem cells for the treatment of streptozotocin induced diabetic rats |
| topic | Mesenchymal stem cell Portal vein Insulin-producing cells Transplant |
| url | http://www.jbiomedsci.com/content/19/1/47 |
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