Meta-Analysis of Gene Expressions in Testicular Germ Cell Tumor Histologies
There is no consensus as to how a precursor lesion, germ cell neoplasia in situ (GCNIS), develops into the histologic types of testicular germ cell tumor type II (TGCT). The present meta-analysis examined RNA expressions of 24 candidate genes in three datasets. They included 203 samples of normal te...
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| Format: | Article |
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MDPI AG
2020-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/12/4487 |
| _version_ | 1797564316722921472 |
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| author | Finn Edler von Eyben Jorge Parraga-Alava |
| author_facet | Finn Edler von Eyben Jorge Parraga-Alava |
| author_sort | Finn Edler von Eyben |
| collection | DOAJ |
| description | There is no consensus as to how a precursor lesion, germ cell neoplasia in situ (GCNIS), develops into the histologic types of testicular germ cell tumor type II (TGCT). The present meta-analysis examined RNA expressions of 24 candidate genes in three datasets. They included 203 samples of normal testis (NT) and histologic types of TGCT. The Fisher’s test for combined <i>p</i> values was used for meta-analysis of the RNA expressions in the three datasets. The histologic types differed in RNA expression of <i>PRAME, KIT, SOX17, NANOG, KLF4, POU5F1, RB1, DNMT3B</i>, and <i>LIN28A</i> (<i>p</i> < 0.01). The histologic types had concordant differences in RNA expression of the genes in the three datasets. Eight genes had overlap with a high RNA expression in at least two histologic types. In contrast, only seminoma (SE) had a high RNA expression of <i>KLF4</i> and only embryonal carcinoma (EC) had a high RNA expression of <i>DNMT3B</i>. In conclusion, the meta-analysis showed that the development of the histologic types of TGCT was driven by changes in RNA expression of candidate genes. According to the RNA expressions of the ten genes, TGCT develops from NT over GCNIS, SE, EC, to the differentiated types of TGCT. |
| first_indexed | 2024-03-10T18:55:36Z |
| format | Article |
| id | doaj.art-b4d2a9f5942c458fa36304716d41ce33 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T18:55:36Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-b4d2a9f5942c458fa36304716d41ce332023-11-20T04:49:12ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-06-012112448710.3390/ijms21124487Meta-Analysis of Gene Expressions in Testicular Germ Cell Tumor HistologiesFinn Edler von Eyben0Jorge Parraga-Alava1Center of Tobacco Control Research, DK-5230 Odense M, DenmarkFacultad de Ciencias Informaticas, Universidad Tecnica de Manabi, Portoviejo 130105, EcuadorThere is no consensus as to how a precursor lesion, germ cell neoplasia in situ (GCNIS), develops into the histologic types of testicular germ cell tumor type II (TGCT). The present meta-analysis examined RNA expressions of 24 candidate genes in three datasets. They included 203 samples of normal testis (NT) and histologic types of TGCT. The Fisher’s test for combined <i>p</i> values was used for meta-analysis of the RNA expressions in the three datasets. The histologic types differed in RNA expression of <i>PRAME, KIT, SOX17, NANOG, KLF4, POU5F1, RB1, DNMT3B</i>, and <i>LIN28A</i> (<i>p</i> < 0.01). The histologic types had concordant differences in RNA expression of the genes in the three datasets. Eight genes had overlap with a high RNA expression in at least two histologic types. In contrast, only seminoma (SE) had a high RNA expression of <i>KLF4</i> and only embryonal carcinoma (EC) had a high RNA expression of <i>DNMT3B</i>. In conclusion, the meta-analysis showed that the development of the histologic types of TGCT was driven by changes in RNA expression of candidate genes. According to the RNA expressions of the ten genes, TGCT develops from NT over GCNIS, SE, EC, to the differentiated types of TGCT.https://www.mdpi.com/1422-0067/21/12/4487biomarkersgenes for pluripotencymeta-analysisoncogenespathogenesisRNA expression |
| spellingShingle | Finn Edler von Eyben Jorge Parraga-Alava Meta-Analysis of Gene Expressions in Testicular Germ Cell Tumor Histologies International Journal of Molecular Sciences biomarkers genes for pluripotency meta-analysis oncogenes pathogenesis RNA expression |
| title | Meta-Analysis of Gene Expressions in Testicular Germ Cell Tumor Histologies |
| title_full | Meta-Analysis of Gene Expressions in Testicular Germ Cell Tumor Histologies |
| title_fullStr | Meta-Analysis of Gene Expressions in Testicular Germ Cell Tumor Histologies |
| title_full_unstemmed | Meta-Analysis of Gene Expressions in Testicular Germ Cell Tumor Histologies |
| title_short | Meta-Analysis of Gene Expressions in Testicular Germ Cell Tumor Histologies |
| title_sort | meta analysis of gene expressions in testicular germ cell tumor histologies |
| topic | biomarkers genes for pluripotency meta-analysis oncogenes pathogenesis RNA expression |
| url | https://www.mdpi.com/1422-0067/21/12/4487 |
| work_keys_str_mv | AT finnedlervoneyben metaanalysisofgeneexpressionsintesticulargermcelltumorhistologies AT jorgeparragaalava metaanalysisofgeneexpressionsintesticulargermcelltumorhistologies |