Single-Oocyte Gene Expression Suggests That Curcumin Can Protect the Ovarian Reserve by Regulating the PTEN-AKT-FOXO3a Pathway
A better understanding of the mechanism of primordial follicle activation will help us better understand the causes of premature ovarian insufficiency (POI), and will help us identify new drugs that can be applied to the clinical treatment of infertility. In this study, single oocytes were isolated...
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MDPI AG
2021-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/12/6570 |
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| author | Yue Lv Rui-Can Cao Hong-Bin Liu Xian-Wei Su Gang Lu Jin-Long Ma Wai-Yee Chan |
| author_facet | Yue Lv Rui-Can Cao Hong-Bin Liu Xian-Wei Su Gang Lu Jin-Long Ma Wai-Yee Chan |
| author_sort | Yue Lv |
| collection | DOAJ |
| description | A better understanding of the mechanism of primordial follicle activation will help us better understand the causes of premature ovarian insufficiency (POI), and will help us identify new drugs that can be applied to the clinical treatment of infertility. In this study, single oocytes were isolated from primordial and primary follicles, and were used for gene profiling with TaqMan array cards. Bioinformatics analysis was performed on the gene expression data, and Ingenuity Pathway Analysis was used to analyze and predict drugs that affect follicle activation. An ovarian in vitro culture system was used to verify the function of the drug candidates, and we found that curcumin maintains the ovarian reserve. Long-term treatment with 100 mg/kg curcumin improved the ovarian reserve indicators of AMH, FSH, and estradiol in aging mice. Mechanistic studies show that curcumin can affect the translocation of FOXO3, thereby inhibiting the PTEN-AKT-FOXO3a pathway and protecting primordial follicles from overactivation. These results suggest that curcumin is a potential drug for the treatment of POI patients and for fertility preservation. |
| first_indexed | 2024-03-10T10:16:50Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T10:16:50Z |
| publishDate | 2021-06-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b4d315dbfe6d4fb49c2558de909d626c2023-11-22T00:45:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012212657010.3390/ijms22126570Single-Oocyte Gene Expression Suggests That Curcumin Can Protect the Ovarian Reserve by Regulating the PTEN-AKT-FOXO3a PathwayYue Lv0Rui-Can Cao1Hong-Bin Liu2Xian-Wei Su3Gang Lu4Jin-Long Ma5Wai-Yee Chan6School of Basic Medical Sciences, Shandong University, Jinan 250012, ChinaCUHK-SDU Joint Laboratory on Reproductive Genetics, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, ChinaCUHK-SDU Joint Laboratory on Reproductive Genetics, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, ChinaCenter for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan 250012, ChinaCUHK-SDU Joint Laboratory on Reproductive Genetics, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, ChinaSchool of Basic Medical Sciences, Shandong University, Jinan 250012, ChinaCUHK-SDU Joint Laboratory on Reproductive Genetics, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, ChinaA better understanding of the mechanism of primordial follicle activation will help us better understand the causes of premature ovarian insufficiency (POI), and will help us identify new drugs that can be applied to the clinical treatment of infertility. In this study, single oocytes were isolated from primordial and primary follicles, and were used for gene profiling with TaqMan array cards. Bioinformatics analysis was performed on the gene expression data, and Ingenuity Pathway Analysis was used to analyze and predict drugs that affect follicle activation. An ovarian in vitro culture system was used to verify the function of the drug candidates, and we found that curcumin maintains the ovarian reserve. Long-term treatment with 100 mg/kg curcumin improved the ovarian reserve indicators of AMH, FSH, and estradiol in aging mice. Mechanistic studies show that curcumin can affect the translocation of FOXO3, thereby inhibiting the PTEN-AKT-FOXO3a pathway and protecting primordial follicles from overactivation. These results suggest that curcumin is a potential drug for the treatment of POI patients and for fertility preservation.https://www.mdpi.com/1422-0067/22/12/6570single cell qPCRprimordial folliclefollicle activationovarian reserveingenuity pathway analysiscurcumin |
| spellingShingle | Yue Lv Rui-Can Cao Hong-Bin Liu Xian-Wei Su Gang Lu Jin-Long Ma Wai-Yee Chan Single-Oocyte Gene Expression Suggests That Curcumin Can Protect the Ovarian Reserve by Regulating the PTEN-AKT-FOXO3a Pathway International Journal of Molecular Sciences single cell qPCR primordial follicle follicle activation ovarian reserve ingenuity pathway analysis curcumin |
| title | Single-Oocyte Gene Expression Suggests That Curcumin Can Protect the Ovarian Reserve by Regulating the PTEN-AKT-FOXO3a Pathway |
| title_full | Single-Oocyte Gene Expression Suggests That Curcumin Can Protect the Ovarian Reserve by Regulating the PTEN-AKT-FOXO3a Pathway |
| title_fullStr | Single-Oocyte Gene Expression Suggests That Curcumin Can Protect the Ovarian Reserve by Regulating the PTEN-AKT-FOXO3a Pathway |
| title_full_unstemmed | Single-Oocyte Gene Expression Suggests That Curcumin Can Protect the Ovarian Reserve by Regulating the PTEN-AKT-FOXO3a Pathway |
| title_short | Single-Oocyte Gene Expression Suggests That Curcumin Can Protect the Ovarian Reserve by Regulating the PTEN-AKT-FOXO3a Pathway |
| title_sort | single oocyte gene expression suggests that curcumin can protect the ovarian reserve by regulating the pten akt foxo3a pathway |
| topic | single cell qPCR primordial follicle follicle activation ovarian reserve ingenuity pathway analysis curcumin |
| url | https://www.mdpi.com/1422-0067/22/12/6570 |
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