RETRACTED: Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer
Many bacterial pathogens can cause septicemia and spread from the bloodstream into internal organs. During leptospirosis, individuals are infected by contact with Leptospira-containing animal urine-contaminated water. The spirochetes invade internal organs after septicemia to cause disease aggravati...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2019-04-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/44594 |
| _version_ | 1797633664987693056 |
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| author | Yang Li Kai-Xuan Li Wei-Lin Hu David M Ojcius Jia-Qi Fang Shi-Jun Li Xu'ai Lin Jie Yan |
| author_facet | Yang Li Kai-Xuan Li Wei-Lin Hu David M Ojcius Jia-Qi Fang Shi-Jun Li Xu'ai Lin Jie Yan |
| author_sort | Yang Li |
| collection | DOAJ |
| description | Many bacterial pathogens can cause septicemia and spread from the bloodstream into internal organs. During leptospirosis, individuals are infected by contact with Leptospira-containing animal urine-contaminated water. The spirochetes invade internal organs after septicemia to cause disease aggravation, but the mechanism of leptospiral excretion and spreading remains unknown. Here, we demonstrated that Leptospira interrogans entered human/mouse endothelial and epithelial cells and fibroblasts by caveolae/integrin-β1-PI3K/FAK-mediated microfilament-dependent endocytosis to form Leptospira (Lep)-vesicles that did not fuse with lysosomes. Lep-vesicles recruited Rab5/Rab11 and Sec/Exo-SNARE proteins in endocytic recycling and vesicular transport systems for intracellular transport and release by SNARE-complex/FAK-mediated microfilament/microtubule-dependent exocytosis. Both intracellular leptospires and infected cells maintained their viability. Leptospiral propagation was only observed in mouse fibroblasts. Our study revealed that L. interrogans utilizes endocytic recycling and vesicular transport systems for transcytosis across endothelial or epithelial barrier in blood vessels or renal tubules, which contributes to spreading in vivo and transmission of leptospirosis. |
| first_indexed | 2024-03-11T11:57:05Z |
| format | Article |
| id | doaj.art-b4d52125be434b2ba34dfd795cdd3112 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-03-11T11:57:05Z |
| publishDate | 2019-04-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-b4d52125be434b2ba34dfd795cdd31122023-11-08T14:19:55ZengeLife Sciences Publications LtdeLife2050-084X2019-04-01810.7554/eLife.44594RETRACTED: Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayerYang Li0Kai-Xuan Li1Wei-Lin Hu2David M Ojcius3Jia-Qi Fang4Shi-Jun Li5Xu'ai Lin6Jie Yan7https://orcid.org/0000-0003-2054-6986Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University School of Medicine, Hangzhou, China; Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, China; Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, China; Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaCollaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University School of Medicine, Hangzhou, China; Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, China; Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Biomedical Sciences, Arthur Dugoni School of Dentistry, University of the Pacific, San Francisco, United StatesDepartment of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, China; Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaInstitute of Communicable Disease Prevention and Control, Guizhou Provincial Centre for Disease Control and Prevention, Guiyang, ChinaCollaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University School of Medicine, Hangzhou, China; Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, China; Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaCollaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University School of Medicine, Hangzhou, China; Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, China; Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaMany bacterial pathogens can cause septicemia and spread from the bloodstream into internal organs. During leptospirosis, individuals are infected by contact with Leptospira-containing animal urine-contaminated water. The spirochetes invade internal organs after septicemia to cause disease aggravation, but the mechanism of leptospiral excretion and spreading remains unknown. Here, we demonstrated that Leptospira interrogans entered human/mouse endothelial and epithelial cells and fibroblasts by caveolae/integrin-β1-PI3K/FAK-mediated microfilament-dependent endocytosis to form Leptospira (Lep)-vesicles that did not fuse with lysosomes. Lep-vesicles recruited Rab5/Rab11 and Sec/Exo-SNARE proteins in endocytic recycling and vesicular transport systems for intracellular transport and release by SNARE-complex/FAK-mediated microfilament/microtubule-dependent exocytosis. Both intracellular leptospires and infected cells maintained their viability. Leptospiral propagation was only observed in mouse fibroblasts. Our study revealed that L. interrogans utilizes endocytic recycling and vesicular transport systems for transcytosis across endothelial or epithelial barrier in blood vessels or renal tubules, which contributes to spreading in vivo and transmission of leptospirosis.https://elifesciences.org/articles/44594leptospirosisLeptospira interroganstranscytosisendocytosisinfection spreadingleptospiral excretion |
| spellingShingle | Yang Li Kai-Xuan Li Wei-Lin Hu David M Ojcius Jia-Qi Fang Shi-Jun Li Xu'ai Lin Jie Yan RETRACTED: Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer eLife leptospirosis Leptospira interrogans transcytosis endocytosis infection spreading leptospiral excretion |
| title | RETRACTED: Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer |
| title_full | RETRACTED: Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer |
| title_fullStr | RETRACTED: Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer |
| title_full_unstemmed | RETRACTED: Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer |
| title_short | RETRACTED: Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer |
| title_sort | retracted endocytic recycling and vesicular transport systems mediate transcytosis of leptospira interrogans across cell monolayer |
| topic | leptospirosis Leptospira interrogans transcytosis endocytosis infection spreading leptospiral excretion |
| url | https://elifesciences.org/articles/44594 |
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