Rapid identification of <it>Aspergillus fumigatus </it>within the section <it>Fumigati</it>
<p>Abstract</p> <p>Background</p> <p>New fungal species that are morphologically similar to <it>Aspergillus fumigatus </it>were recently described and included in section <it>Fumigati</it>. Misidentification of such fungal species, particularly o...
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| Format: | Article |
| Language: | English |
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BMC
2011-04-01
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| Series: | BMC Microbiology |
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| Online Access: | http://www.biomedcentral.com/1471-2180/11/82 |
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| author | Amorim António Gusmão Leonor Serrano Rita Araujo Ricardo |
| author_facet | Amorim António Gusmão Leonor Serrano Rita Araujo Ricardo |
| author_sort | Amorim António |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>New fungal species that are morphologically similar to <it>Aspergillus fumigatus </it>were recently described and included in section <it>Fumigati</it>. Misidentification of such fungal species, particularly of the human pathogens, <it>Aspergillus lentulus</it>, <it>Neosartorya fischeri</it>, <it>Neosartorya hiratsukae</it>, <it>Neosartorya pseudofischeri </it>and <it>Neosartorya udagawae</it>, has been increasingly reported by numerous clinical labs. Nevertheless, <it>A. fumigatus </it>still accounts for more than 90% of all invasive aspergillosis cases. The purpose of the present study was to develop a rapid method for the molecular identification of <it>A. fumigatus </it>to distinguish it from other species within the section <it>Fumigati</it>.</p> <p>Results</p> <p>A multiplex PCR was developed using prior information based on β-tubulin (βtub) and rodlet A (rodA) partial gene sequences. PCR amplification of βtub and rodA fragments resulted in a distinctive electrophoretic pattern in <it>A. fumigatus </it>and <it>N. udagawae</it>. The polymorphisms found in the smallest amplified sequence of βtub (153 bp) and rodA (103 bp) genes were then compared among and within species of this taxonomic section. βtub was able to differentiate among 13 individual species and two groups of species that included the pathogenic fungus <it>A. lentulus</it>. A more limited number of sequences were available for rodA; nevertheless, we were able to distinguish <it>Aspergillus viridinutans, N. hiratsukae </it>and <it>N. udagawae</it>.</p> <p>Conclusions</p> <p>The assay described in the present study proved to be specific and highly reproducible, representing a fast and economic way of targeting molecular identification of the relevant mould, <it>A. fumigatus</it>, in clinical laboratories.</p> |
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| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-19T02:06:58Z |
| publishDate | 2011-04-01 |
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| spelling | doaj.art-b4d65c0823a7431fb7777ae97778b5852022-12-21T20:40:54ZengBMCBMC Microbiology1471-21802011-04-011118210.1186/1471-2180-11-82Rapid identification of <it>Aspergillus fumigatus </it>within the section <it>Fumigati</it>Amorim AntónioGusmão LeonorSerrano RitaAraujo Ricardo<p>Abstract</p> <p>Background</p> <p>New fungal species that are morphologically similar to <it>Aspergillus fumigatus </it>were recently described and included in section <it>Fumigati</it>. Misidentification of such fungal species, particularly of the human pathogens, <it>Aspergillus lentulus</it>, <it>Neosartorya fischeri</it>, <it>Neosartorya hiratsukae</it>, <it>Neosartorya pseudofischeri </it>and <it>Neosartorya udagawae</it>, has been increasingly reported by numerous clinical labs. Nevertheless, <it>A. fumigatus </it>still accounts for more than 90% of all invasive aspergillosis cases. The purpose of the present study was to develop a rapid method for the molecular identification of <it>A. fumigatus </it>to distinguish it from other species within the section <it>Fumigati</it>.</p> <p>Results</p> <p>A multiplex PCR was developed using prior information based on β-tubulin (βtub) and rodlet A (rodA) partial gene sequences. PCR amplification of βtub and rodA fragments resulted in a distinctive electrophoretic pattern in <it>A. fumigatus </it>and <it>N. udagawae</it>. The polymorphisms found in the smallest amplified sequence of βtub (153 bp) and rodA (103 bp) genes were then compared among and within species of this taxonomic section. βtub was able to differentiate among 13 individual species and two groups of species that included the pathogenic fungus <it>A. lentulus</it>. A more limited number of sequences were available for rodA; nevertheless, we were able to distinguish <it>Aspergillus viridinutans, N. hiratsukae </it>and <it>N. udagawae</it>.</p> <p>Conclusions</p> <p>The assay described in the present study proved to be specific and highly reproducible, representing a fast and economic way of targeting molecular identification of the relevant mould, <it>A. fumigatus</it>, in clinical laboratories.</p>http://www.biomedcentral.com/1471-2180/11/82<it>Aspergillus</it>azole resistanceelectrophoretic profileinvasive aspergillosismolecular identificationmouldmultiplex PCRrodlet Aβ-tubulin |
| spellingShingle | Amorim António Gusmão Leonor Serrano Rita Araujo Ricardo Rapid identification of <it>Aspergillus fumigatus </it>within the section <it>Fumigati</it> BMC Microbiology <it>Aspergillus</it> azole resistance electrophoretic profile invasive aspergillosis molecular identification mould multiplex PCR rodlet A β-tubulin |
| title | Rapid identification of <it>Aspergillus fumigatus </it>within the section <it>Fumigati</it> |
| title_full | Rapid identification of <it>Aspergillus fumigatus </it>within the section <it>Fumigati</it> |
| title_fullStr | Rapid identification of <it>Aspergillus fumigatus </it>within the section <it>Fumigati</it> |
| title_full_unstemmed | Rapid identification of <it>Aspergillus fumigatus </it>within the section <it>Fumigati</it> |
| title_short | Rapid identification of <it>Aspergillus fumigatus </it>within the section <it>Fumigati</it> |
| title_sort | rapid identification of it aspergillus fumigatus it within the section it fumigati it |
| topic | <it>Aspergillus</it> azole resistance electrophoretic profile invasive aspergillosis molecular identification mould multiplex PCR rodlet A β-tubulin |
| url | http://www.biomedcentral.com/1471-2180/11/82 |
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