Comparative genomics of regulation of heavy metal resistance in Eubacteria
<p>Abstract</p> <p>Background</p> <p>Heavy metal resistance (HMR) in Eubacteria is regulated by a variety of systems including transcription factors from the MerR family (COG0789). The HMR systems are characterized by the complex signal structure (strong palindrome with...
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| Format: | Article |
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BMC
2006-06-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/6/49 |
| _version_ | 1811310997403598848 |
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| author | Kalinina OV Kazakov AE Permina EA Gelfand MS |
| author_facet | Kalinina OV Kazakov AE Permina EA Gelfand MS |
| author_sort | Kalinina OV |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Heavy metal resistance (HMR) in Eubacteria is regulated by a variety of systems including transcription factors from the MerR family (COG0789). The HMR systems are characterized by the complex signal structure (strong palindrome within a 19 or 20 bp promoter spacer), and usually consist of transporter and regulator genes. Some HMR regulons also include detoxification systems. The number of sequenced bacterial genomes is constantly increasing and even though HMR resistance regulons of the COG0789 type usually consist of few genes per genome, the computational analysis may contribute to the understanding of the cellular systems of metal detoxification.</p> <p>Results</p> <p>We studied the mercury (MerR), copper (CueR and HmrR), cadmium (CadR), lead (PbrR), and zinc (ZntR) resistance systems and demonstrated that combining protein sequence analysis and analysis of DNA regulatory signals it was possible to distinguish metal-dependent members of COG0789, assign specificity towards particular metals to uncharacterized loci, and find new genes involved in the metal resistance, in particular, multicopper oxidase and copper chaperones, candidate cytochromes from the copper regulon, new cadmium transporters and, possibly, glutathione-S-transferases.</p> <p>Conclusion</p> <p>Our data indicate that the specificity of the COG0789 systems can be determined combining phylogenetic analysis and identification of DNA regulatory sites. Taking into account signal structure, we can adequately identify genes that are activated using the DNA bending-unbending mechanism. In the case of regulon members that do not reside in single loci, analysis of potential regulatory sites could be crucial for the correct annotation and prediction of the specificity.</p> |
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| format | Article |
| id | doaj.art-b4db06a70feb4be9bcd8400a9b647d0a |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-13T10:10:06Z |
| publishDate | 2006-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-b4db06a70feb4be9bcd8400a9b647d0a2022-12-22T02:50:58ZengBMCBMC Microbiology1471-21802006-06-01614910.1186/1471-2180-6-49Comparative genomics of regulation of heavy metal resistance in EubacteriaKalinina OVKazakov AEPermina EAGelfand MS<p>Abstract</p> <p>Background</p> <p>Heavy metal resistance (HMR) in Eubacteria is regulated by a variety of systems including transcription factors from the MerR family (COG0789). The HMR systems are characterized by the complex signal structure (strong palindrome within a 19 or 20 bp promoter spacer), and usually consist of transporter and regulator genes. Some HMR regulons also include detoxification systems. The number of sequenced bacterial genomes is constantly increasing and even though HMR resistance regulons of the COG0789 type usually consist of few genes per genome, the computational analysis may contribute to the understanding of the cellular systems of metal detoxification.</p> <p>Results</p> <p>We studied the mercury (MerR), copper (CueR and HmrR), cadmium (CadR), lead (PbrR), and zinc (ZntR) resistance systems and demonstrated that combining protein sequence analysis and analysis of DNA regulatory signals it was possible to distinguish metal-dependent members of COG0789, assign specificity towards particular metals to uncharacterized loci, and find new genes involved in the metal resistance, in particular, multicopper oxidase and copper chaperones, candidate cytochromes from the copper regulon, new cadmium transporters and, possibly, glutathione-S-transferases.</p> <p>Conclusion</p> <p>Our data indicate that the specificity of the COG0789 systems can be determined combining phylogenetic analysis and identification of DNA regulatory sites. Taking into account signal structure, we can adequately identify genes that are activated using the DNA bending-unbending mechanism. In the case of regulon members that do not reside in single loci, analysis of potential regulatory sites could be crucial for the correct annotation and prediction of the specificity.</p>http://www.biomedcentral.com/1471-2180/6/49 |
| spellingShingle | Kalinina OV Kazakov AE Permina EA Gelfand MS Comparative genomics of regulation of heavy metal resistance in Eubacteria BMC Microbiology |
| title | Comparative genomics of regulation of heavy metal resistance in Eubacteria |
| title_full | Comparative genomics of regulation of heavy metal resistance in Eubacteria |
| title_fullStr | Comparative genomics of regulation of heavy metal resistance in Eubacteria |
| title_full_unstemmed | Comparative genomics of regulation of heavy metal resistance in Eubacteria |
| title_short | Comparative genomics of regulation of heavy metal resistance in Eubacteria |
| title_sort | comparative genomics of regulation of heavy metal resistance in eubacteria |
| url | http://www.biomedcentral.com/1471-2180/6/49 |
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