A modified surgical procedure using minimally invasive ileocolic vein perfusion in a mouse intrahepatic islet transplant model
Summary: Murine intrahepatic islet transplantation is a clinically relevant but technically challenging surgical procedure because of frequent lethal postoperative bleeding. Here, we describe a protocol for mouse pancreatic islet isolation, purification, and culture. Besides, we also describe a prot...
Main Authors: | Lei Zhang, Ali Turan, Haval Shirwan, Esma S. Yolcu |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2022-06-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722002969 |
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