Exploring Antimicrobial Resistance Profiles of <i>E. coli</i> Isolates in Dairy Cattle: A Baseline Study across Dairy Farms with Varied Husbandry Practices in Puerto Rico
Antimicrobial treatment in livestock can contribute to the emergence and spread of antimicrobial-resistant (AMR) microorganisms. Despite substantial surveillance of AMR bacteria in the continental United States, the prevalence of these AMR organisms in U.S. territories, such as Puerto Rico, remains...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/12/2879 |
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| author | Yadira Malavez Sharon M. Nieves-Miranda Paola N. Loperena Gonzalez Adrian F. Padin-Lopez Lingzi Xiaoli Edward G. Dudley |
| author_facet | Yadira Malavez Sharon M. Nieves-Miranda Paola N. Loperena Gonzalez Adrian F. Padin-Lopez Lingzi Xiaoli Edward G. Dudley |
| author_sort | Yadira Malavez |
| collection | DOAJ |
| description | Antimicrobial treatment in livestock can contribute to the emergence and spread of antimicrobial-resistant (AMR) microorganisms. Despite substantial surveillance of AMR bacteria in the continental United States, the prevalence of these AMR organisms in U.S. territories, such as Puerto Rico, remains understudied. The goals of this research included obtaining baseline data on the antimicrobial profile of <i>E. coli</i> isolates from Puerto Rico dairy farms with different husbandry practices. Seventy-nine fecal samples were collected from two types of conventional dairy farms: those that fed calves with tank milk and those that fed calves with waste milk. These samples were collected from the animals’ rectums, culture, and subsequently confirmed through biochemical tests. Out of these samples, 32 isolates were analyzed phenotypically and genotypically to elucidate their AMR profiles. The results underscore a discrepancy in the occurrence of antimicrobial resistance genes between calves and adult cattle. Notably, waste milk-fed calves exhibited a significantly higher prevalence of antibiotic-resistant <i>E. coli</i> when compared to their tank milk-fed counterparts. These disparities emphasize the need for more comprehensive investigations to determine causative factors. These results underscore the urgency of comprehensive strategies to raise awareness about how management practices influence antimicrobial resistance, shifting the focus from treatment to prevention. |
| first_indexed | 2024-03-08T20:31:13Z |
| format | Article |
| id | doaj.art-b4e1dc4de2314105976c6915988335f0 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-08T20:31:13Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-b4e1dc4de2314105976c6915988335f02023-12-22T14:25:52ZengMDPI AGMicroorganisms2076-26072023-11-011112287910.3390/microorganisms11122879Exploring Antimicrobial Resistance Profiles of <i>E. coli</i> Isolates in Dairy Cattle: A Baseline Study across Dairy Farms with Varied Husbandry Practices in Puerto RicoYadira Malavez0Sharon M. Nieves-Miranda1Paola N. Loperena Gonzalez2Adrian F. Padin-Lopez3Lingzi Xiaoli4Edward G. Dudley5Department of Natural Sciences, University of Puerto Rico, Aguadilla, PR 00603, USADepartment of Natural Sciences, University of Puerto Rico, Aguadilla, PR 00603, USADepartment of Natural Sciences, University of Puerto Rico, Aguadilla, PR 00603, USADepartment of Natural Sciences, University of Puerto Rico, Aguadilla, PR 00603, USADepartment of Food Science, The Pennsylvania State University, University Park, PA 16802, USADepartment of Food Science, The Pennsylvania State University, University Park, PA 16802, USAAntimicrobial treatment in livestock can contribute to the emergence and spread of antimicrobial-resistant (AMR) microorganisms. Despite substantial surveillance of AMR bacteria in the continental United States, the prevalence of these AMR organisms in U.S. territories, such as Puerto Rico, remains understudied. The goals of this research included obtaining baseline data on the antimicrobial profile of <i>E. coli</i> isolates from Puerto Rico dairy farms with different husbandry practices. Seventy-nine fecal samples were collected from two types of conventional dairy farms: those that fed calves with tank milk and those that fed calves with waste milk. These samples were collected from the animals’ rectums, culture, and subsequently confirmed through biochemical tests. Out of these samples, 32 isolates were analyzed phenotypically and genotypically to elucidate their AMR profiles. The results underscore a discrepancy in the occurrence of antimicrobial resistance genes between calves and adult cattle. Notably, waste milk-fed calves exhibited a significantly higher prevalence of antibiotic-resistant <i>E. coli</i> when compared to their tank milk-fed counterparts. These disparities emphasize the need for more comprehensive investigations to determine causative factors. These results underscore the urgency of comprehensive strategies to raise awareness about how management practices influence antimicrobial resistance, shifting the focus from treatment to prevention.https://www.mdpi.com/2076-2607/11/12/2879antimicrobial resistancedairy farms<i>E. coli</i>Puerto Ricowhole-genome sequencing |
| spellingShingle | Yadira Malavez Sharon M. Nieves-Miranda Paola N. Loperena Gonzalez Adrian F. Padin-Lopez Lingzi Xiaoli Edward G. Dudley Exploring Antimicrobial Resistance Profiles of <i>E. coli</i> Isolates in Dairy Cattle: A Baseline Study across Dairy Farms with Varied Husbandry Practices in Puerto Rico Microorganisms antimicrobial resistance dairy farms <i>E. coli</i> Puerto Rico whole-genome sequencing |
| title | Exploring Antimicrobial Resistance Profiles of <i>E. coli</i> Isolates in Dairy Cattle: A Baseline Study across Dairy Farms with Varied Husbandry Practices in Puerto Rico |
| title_full | Exploring Antimicrobial Resistance Profiles of <i>E. coli</i> Isolates in Dairy Cattle: A Baseline Study across Dairy Farms with Varied Husbandry Practices in Puerto Rico |
| title_fullStr | Exploring Antimicrobial Resistance Profiles of <i>E. coli</i> Isolates in Dairy Cattle: A Baseline Study across Dairy Farms with Varied Husbandry Practices in Puerto Rico |
| title_full_unstemmed | Exploring Antimicrobial Resistance Profiles of <i>E. coli</i> Isolates in Dairy Cattle: A Baseline Study across Dairy Farms with Varied Husbandry Practices in Puerto Rico |
| title_short | Exploring Antimicrobial Resistance Profiles of <i>E. coli</i> Isolates in Dairy Cattle: A Baseline Study across Dairy Farms with Varied Husbandry Practices in Puerto Rico |
| title_sort | exploring antimicrobial resistance profiles of i e coli i isolates in dairy cattle a baseline study across dairy farms with varied husbandry practices in puerto rico |
| topic | antimicrobial resistance dairy farms <i>E. coli</i> Puerto Rico whole-genome sequencing |
| url | https://www.mdpi.com/2076-2607/11/12/2879 |
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