Mass Spectrometric Studies on the Addition Reaction of HCCA and Sulfhydryl-Containing Living Substance in MALDI-TOF MS Analysis
The amuch eaddition reactions between the common matrix α-cyano-4-hydroxy cinnamic acid (HCCA) in matrix assisted laser desorption (MALDI) and the sulfhydryl-contained compounds were investigated using matrix assisted laser desorption time of flight mass spectrometry (MALDI-TOF MS) and electrospray...
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Editorial Board of Journal of Chinese Mass Spectrometry Society
2022-03-01
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| Series: | Zhipu Xuebao |
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| Online Access: | http://www.jcmss.com.cn/CN/article/openArticlePDF.jsp?attachType=PDF&id=3054 |
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| author | LI Jing1,2 REN Qi-long1,2 PAN Yuan-jiang3 |
| author_facet | LI Jing1,2 REN Qi-long1,2 PAN Yuan-jiang3 |
| author_sort | LI Jing1,2 |
| collection | DOAJ |
| description | The amuch eaddition reactions between the common matrix α-cyano-4-hydroxy cinnamic acid (HCCA) in matrix assisted laser desorption (MALDI) and the sulfhydryl-contained compounds were investigated using matrix assisted laser desorption time of flight mass spectrometry (MALDI-TOF MS) and electrospray mass spectrometry (ESI-MS), and the corresponding reactivity was also compared in the acid, weak acid and alkaline conditions, respectively. The results showed that the sulfhydryl-contained compounds could much easily undergo addition reaction with HCCA under alkaline conditions, resulting in a mass shift of 189.0 u in the mass spectrum. In addition, the identity of the reaction product was supported by high resolution mass spectrometry analysis, nd was further identified as covalent bonding products by tandem MS analysis. This work provides a proof for the addition reaction between MALDI matrix and sulfhydryl-contained compounds, so as to reduce the interference of the matrix in the MALDI-TOF MS analysis of the relevant compounds, and to provide a reference for the selection of matrix. |
| first_indexed | 2024-12-13T07:26:54Z |
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| id | doaj.art-b4e29a359da34cbf8446e7a16cf67afd |
| institution | Directory Open Access Journal |
| issn | 1004-2997 |
| language | English |
| last_indexed | 2024-12-13T07:26:54Z |
| publishDate | 2022-03-01 |
| publisher | Editorial Board of Journal of Chinese Mass Spectrometry Society |
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| series | Zhipu Xuebao |
| spelling | doaj.art-b4e29a359da34cbf8446e7a16cf67afd2022-12-21T23:55:18ZengEditorial Board of Journal of Chinese Mass Spectrometry SocietyZhipu Xuebao1004-29972022-03-0143213314110.7538/zpxb.2021.0064Mass Spectrometric Studies on the Addition Reaction of HCCA and Sulfhydryl-Containing Living Substance in MALDI-TOF MS AnalysisLI Jing1,20REN Qi-long1,21PAN Yuan-jiang321.Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University;2. Institute of Zhejiang University Quzhou1.Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University;2. Institute of Zhejiang University Quzhou3.Department of Chemistry, Zhejiang UniversityThe amuch eaddition reactions between the common matrix α-cyano-4-hydroxy cinnamic acid (HCCA) in matrix assisted laser desorption (MALDI) and the sulfhydryl-contained compounds were investigated using matrix assisted laser desorption time of flight mass spectrometry (MALDI-TOF MS) and electrospray mass spectrometry (ESI-MS), and the corresponding reactivity was also compared in the acid, weak acid and alkaline conditions, respectively. The results showed that the sulfhydryl-contained compounds could much easily undergo addition reaction with HCCA under alkaline conditions, resulting in a mass shift of 189.0 u in the mass spectrum. In addition, the identity of the reaction product was supported by high resolution mass spectrometry analysis, nd was further identified as covalent bonding products by tandem MS analysis. This work provides a proof for the addition reaction between MALDI matrix and sulfhydryl-contained compounds, so as to reduce the interference of the matrix in the MALDI-TOF MS analysis of the relevant compounds, and to provide a reference for the selection of matrix.http://www.jcmss.com.cn/CN/article/openArticlePDF.jsp?attachType=PDF&id=3054matrix assisted laser desorption time of flight mass spectrometry (maldi-tof ms)electrospray mass spectrometry (esi-ms)α-cyano-4-hydroxycinnamic acid (hcca)analyte-matrix adductsulfhydryl-containing living substancefragmentation mechanism |
| spellingShingle | LI Jing1,2 REN Qi-long1,2 PAN Yuan-jiang3 Mass Spectrometric Studies on the Addition Reaction of HCCA and Sulfhydryl-Containing Living Substance in MALDI-TOF MS Analysis Zhipu Xuebao matrix assisted laser desorption time of flight mass spectrometry (maldi-tof ms) electrospray mass spectrometry (esi-ms) α-cyano-4-hydroxycinnamic acid (hcca) analyte-matrix adduct sulfhydryl-containing living substance fragmentation mechanism |
| title | Mass Spectrometric Studies on the Addition Reaction of HCCA and Sulfhydryl-Containing Living Substance in MALDI-TOF MS Analysis |
| title_full | Mass Spectrometric Studies on the Addition Reaction of HCCA and Sulfhydryl-Containing Living Substance in MALDI-TOF MS Analysis |
| title_fullStr | Mass Spectrometric Studies on the Addition Reaction of HCCA and Sulfhydryl-Containing Living Substance in MALDI-TOF MS Analysis |
| title_full_unstemmed | Mass Spectrometric Studies on the Addition Reaction of HCCA and Sulfhydryl-Containing Living Substance in MALDI-TOF MS Analysis |
| title_short | Mass Spectrometric Studies on the Addition Reaction of HCCA and Sulfhydryl-Containing Living Substance in MALDI-TOF MS Analysis |
| title_sort | mass spectrometric studies on the addition reaction of hcca and sulfhydryl containing living substance in maldi tof ms analysis |
| topic | matrix assisted laser desorption time of flight mass spectrometry (maldi-tof ms) electrospray mass spectrometry (esi-ms) α-cyano-4-hydroxycinnamic acid (hcca) analyte-matrix adduct sulfhydryl-containing living substance fragmentation mechanism |
| url | http://www.jcmss.com.cn/CN/article/openArticlePDF.jsp?attachType=PDF&id=3054 |
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