Reactive Nitrogen and Oxygen Intermediates in Patients with Cutaneous Leishmaniasis
The metabolisms of reactive nitrogen and oxygen intermediates (RNI and ROI) in patients with cutaneous leishmaniasis (CL) were investigated and compared with those of healthy subjects. To determine RNI metabolism, nitrite plus nitrate concentrations were measured spectrophotometrically. Nitrite conc...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
1999-03-01
|
| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761999000200009 |
| _version_ | 1797704814148190208 |
|---|---|
| author | Ozcan Erel Abdurrahim Kocyigit Vedat Bulut Mehmet Salih Gurel |
| author_facet | Ozcan Erel Abdurrahim Kocyigit Vedat Bulut Mehmet Salih Gurel |
| author_sort | Ozcan Erel |
| collection | DOAJ |
| description | The metabolisms of reactive nitrogen and oxygen intermediates (RNI and ROI) in patients with cutaneous leishmaniasis (CL) were investigated and compared with those of healthy subjects. To determine RNI metabolism, nitrite plus nitrate concentrations were measured spectrophotometrically. Nitrite concentration in plasma was determined directly by the Griess method. Nitrate levels in plasma were measured after reduction into nitrite by using copper-cadmium-zinc. ROI metabolism was evaluated by measuring erythrocyte superoxide dismutase, catalase and glutathione peroxidase activities. Plasma nitrite plus nitrate levels and erythrocyte superoxide dismutase activity were higher in the patient group than healthy subjects (p<0.01). In contrast, erythrocyte catalase and glutathione peroxidase activities were lower (p<0.05, p<0.01, respectively). ROI metabolism was altered in relation to hydrogen peroxide elevation in patients with CL. These alterations in ROI enable nitric oxide (NO) to amplify its leishmanicidal effect. The determination of ROI and RNI in patients with CL may be a useful tool to evaluate effector mechanisms of NO and clinical manifestations. |
| first_indexed | 2024-03-12T05:26:04Z |
| format | Article |
| id | doaj.art-b4e376f3a3eb48f9b3fb9478a02ad01a |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T05:26:04Z |
| publishDate | 1999-03-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-b4e376f3a3eb48f9b3fb9478a02ad01a2023-09-03T07:24:28ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601999-03-0194217918310.1590/S0074-02761999000200009Reactive Nitrogen and Oxygen Intermediates in Patients with Cutaneous LeishmaniasisOzcan ErelAbdurrahim KocyigitVedat BulutMehmet Salih GurelThe metabolisms of reactive nitrogen and oxygen intermediates (RNI and ROI) in patients with cutaneous leishmaniasis (CL) were investigated and compared with those of healthy subjects. To determine RNI metabolism, nitrite plus nitrate concentrations were measured spectrophotometrically. Nitrite concentration in plasma was determined directly by the Griess method. Nitrate levels in plasma were measured after reduction into nitrite by using copper-cadmium-zinc. ROI metabolism was evaluated by measuring erythrocyte superoxide dismutase, catalase and glutathione peroxidase activities. Plasma nitrite plus nitrate levels and erythrocyte superoxide dismutase activity were higher in the patient group than healthy subjects (p<0.01). In contrast, erythrocyte catalase and glutathione peroxidase activities were lower (p<0.05, p<0.01, respectively). ROI metabolism was altered in relation to hydrogen peroxide elevation in patients with CL. These alterations in ROI enable nitric oxide (NO) to amplify its leishmanicidal effect. The determination of ROI and RNI in patients with CL may be a useful tool to evaluate effector mechanisms of NO and clinical manifestations.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761999000200009nitric oxidesuperoxide dismutasecatalaseglutathione peroxidaseleishmaniasis |
| spellingShingle | Ozcan Erel Abdurrahim Kocyigit Vedat Bulut Mehmet Salih Gurel Reactive Nitrogen and Oxygen Intermediates in Patients with Cutaneous Leishmaniasis Memorias do Instituto Oswaldo Cruz nitric oxide superoxide dismutase catalase glutathione peroxidase leishmaniasis |
| title | Reactive Nitrogen and Oxygen Intermediates in Patients with Cutaneous Leishmaniasis |
| title_full | Reactive Nitrogen and Oxygen Intermediates in Patients with Cutaneous Leishmaniasis |
| title_fullStr | Reactive Nitrogen and Oxygen Intermediates in Patients with Cutaneous Leishmaniasis |
| title_full_unstemmed | Reactive Nitrogen and Oxygen Intermediates in Patients with Cutaneous Leishmaniasis |
| title_short | Reactive Nitrogen and Oxygen Intermediates in Patients with Cutaneous Leishmaniasis |
| title_sort | reactive nitrogen and oxygen intermediates in patients with cutaneous leishmaniasis |
| topic | nitric oxide superoxide dismutase catalase glutathione peroxidase leishmaniasis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761999000200009 |
| work_keys_str_mv | AT ozcanerel reactivenitrogenandoxygenintermediatesinpatientswithcutaneousleishmaniasis AT abdurrahimkocyigit reactivenitrogenandoxygenintermediatesinpatientswithcutaneousleishmaniasis AT vedatbulut reactivenitrogenandoxygenintermediatesinpatientswithcutaneousleishmaniasis AT mehmetsalihgurel reactivenitrogenandoxygenintermediatesinpatientswithcutaneousleishmaniasis |