Structural Shifts in the Soil Prokaryotic Communities Marking the Podzol-Forming Process on Sand Dumps
This work describes the microbial community structure of the continuously revegetated chronosequence of a former sand quarry, which demonstrates a unique example of nearly complete soil restoration in less than 100 years. Samples were collected at five time points (0, 3, 30, 70 years and mature soil...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Soil Systems |
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| Online Access: | https://www.mdpi.com/2571-8789/8/1/9 |
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| author | Elizaveta Evdokimova Ekaterina Ivanova Grigory Gladkov Aleksei Zverev Anastasiia Kimeklis Elena Serikova Alexandr Pinaev Arina Kichko Tatiana Aksenova Evgeny Andronov Evgeny Abakumov |
| author_facet | Elizaveta Evdokimova Ekaterina Ivanova Grigory Gladkov Aleksei Zverev Anastasiia Kimeklis Elena Serikova Alexandr Pinaev Arina Kichko Tatiana Aksenova Evgeny Andronov Evgeny Abakumov |
| author_sort | Elizaveta Evdokimova |
| collection | DOAJ |
| description | This work describes the microbial community structure of the continuously revegetated chronosequence of a former sand quarry, which demonstrates a unique example of nearly complete soil restoration in less than 100 years. Samples were collected at five time points (0, 3, 30, 70 years and mature soil) from the entire set of soil horizons, revealing the history of pedogenesis. Real-time PCR was applied to quantitatively describe the bacterial and archaeal communities. High-throughput sequencing of the bacterial and archaeal V4 variable region of the 16S rRNA gene was used to identify abundant microbial taxa. A beta-diversity analysis revealed that the prokaryotic community structure responded strongly to the processes of organic matter accumulation and the corresponding evolution of the soil into discrete horizons. Changes in soil microbiota in the course of soil profile evolution revealed three groups of prokaryotes, which tended to accumulate in the specific soil horizons and might be associated with the certain soil-forming processes, including plant roots growth. This research showed the heuristic potential of soil horizon profiling in microbiological studies as opposed to the formal depth-dependent separation of the soil layers. The results allowed us to trace the relationship between the structure of the soil prokaryotic community and the peculiarities of the evolution of the podzolic soil profile as well as to identify the microbial indicators and drivers of primary pedogenesis. |
| first_indexed | 2024-04-24T17:49:46Z |
| format | Article |
| id | doaj.art-b4e3b41b1de44ab9be1b2b375d974cf1 |
| institution | Directory Open Access Journal |
| issn | 2571-8789 |
| language | English |
| last_indexed | 2024-04-24T17:49:46Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Soil Systems |
| spelling | doaj.art-b4e3b41b1de44ab9be1b2b375d974cf12024-03-27T14:04:48ZengMDPI AGSoil Systems2571-87892024-01-0181910.3390/soilsystems8010009Structural Shifts in the Soil Prokaryotic Communities Marking the Podzol-Forming Process on Sand DumpsElizaveta Evdokimova0Ekaterina Ivanova1Grigory Gladkov2Aleksei Zverev3Anastasiia Kimeklis4Elena Serikova5Alexandr Pinaev6Arina Kichko7Tatiana Aksenova8Evgeny Andronov9Evgeny Abakumov10All-Russia Research Institute for Agricultural Microbiology (ARRIAM), Podbelsky Chausse 3, Pushkin 8, Saint-Petersburg 196608, RussiaAll-Russia Research Institute for Agricultural Microbiology (ARRIAM), Podbelsky Chausse 3, Pushkin 8, Saint-Petersburg 196608, RussiaAll-Russia Research Institute for Agricultural Microbiology (ARRIAM), Podbelsky Chausse 3, Pushkin 8, Saint-Petersburg 196608, RussiaAll-Russia Research Institute for Agricultural Microbiology (ARRIAM), Podbelsky Chausse 3, Pushkin 8, Saint-Petersburg 196608, RussiaAll-Russia Research Institute for Agricultural Microbiology (ARRIAM), Podbelsky Chausse 3, Pushkin 8, Saint-Petersburg 196608, RussiaAll-Russia Research Institute for Agricultural Microbiology (ARRIAM), Podbelsky Chausse 3, Pushkin 8, Saint-Petersburg 196608, RussiaAll-Russia Research Institute for Agricultural Microbiology (ARRIAM), Podbelsky Chausse 3, Pushkin 8, Saint-Petersburg 196608, RussiaAll-Russia Research Institute for Agricultural Microbiology (ARRIAM), Podbelsky Chausse 3, Pushkin 8, Saint-Petersburg 196608, RussiaAll-Russia Research Institute for Agricultural Microbiology (ARRIAM), Podbelsky Chausse 3, Pushkin 8, Saint-Petersburg 196608, RussiaAll-Russia Research Institute for Agricultural Microbiology (ARRIAM), Podbelsky Chausse 3, Pushkin 8, Saint-Petersburg 196608, RussiaDepartment of Applied Ecology, Faculty of Biology, St. Petersburg State University, 16th Liniya V.O., 29, Saint-Petersburg 199178, RussiaThis work describes the microbial community structure of the continuously revegetated chronosequence of a former sand quarry, which demonstrates a unique example of nearly complete soil restoration in less than 100 years. Samples were collected at five time points (0, 3, 30, 70 years and mature soil) from the entire set of soil horizons, revealing the history of pedogenesis. Real-time PCR was applied to quantitatively describe the bacterial and archaeal communities. High-throughput sequencing of the bacterial and archaeal V4 variable region of the 16S rRNA gene was used to identify abundant microbial taxa. A beta-diversity analysis revealed that the prokaryotic community structure responded strongly to the processes of organic matter accumulation and the corresponding evolution of the soil into discrete horizons. Changes in soil microbiota in the course of soil profile evolution revealed three groups of prokaryotes, which tended to accumulate in the specific soil horizons and might be associated with the certain soil-forming processes, including plant roots growth. This research showed the heuristic potential of soil horizon profiling in microbiological studies as opposed to the formal depth-dependent separation of the soil layers. The results allowed us to trace the relationship between the structure of the soil prokaryotic community and the peculiarities of the evolution of the podzolic soil profile as well as to identify the microbial indicators and drivers of primary pedogenesis.https://www.mdpi.com/2571-8789/8/1/9chronosequencehigh-throughput sequencingmicrobiomesoilsoil horizons16S rRNA gene |
| spellingShingle | Elizaveta Evdokimova Ekaterina Ivanova Grigory Gladkov Aleksei Zverev Anastasiia Kimeklis Elena Serikova Alexandr Pinaev Arina Kichko Tatiana Aksenova Evgeny Andronov Evgeny Abakumov Structural Shifts in the Soil Prokaryotic Communities Marking the Podzol-Forming Process on Sand Dumps Soil Systems chronosequence high-throughput sequencing microbiome soil soil horizons 16S rRNA gene |
| title | Structural Shifts in the Soil Prokaryotic Communities Marking the Podzol-Forming Process on Sand Dumps |
| title_full | Structural Shifts in the Soil Prokaryotic Communities Marking the Podzol-Forming Process on Sand Dumps |
| title_fullStr | Structural Shifts in the Soil Prokaryotic Communities Marking the Podzol-Forming Process on Sand Dumps |
| title_full_unstemmed | Structural Shifts in the Soil Prokaryotic Communities Marking the Podzol-Forming Process on Sand Dumps |
| title_short | Structural Shifts in the Soil Prokaryotic Communities Marking the Podzol-Forming Process on Sand Dumps |
| title_sort | structural shifts in the soil prokaryotic communities marking the podzol forming process on sand dumps |
| topic | chronosequence high-throughput sequencing microbiome soil soil horizons 16S rRNA gene |
| url | https://www.mdpi.com/2571-8789/8/1/9 |
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