Multiple drug resistance of Listeria monocytogenes isolated from aborted women by using serological and molecular techniques in Diwaniyah city/ Iraq
Background and Objectives: The study was sought to detect the effect of Listeria monocytogenes on pregnant Iraqi women at Al-Diwaniya hospitals and determination of virulence genes and antimicrobial susceptibility of isolates. Materials and Methods: 360 specimens including blood, urine, vaginal and...
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
Tehran University of Medical Sciences
2020-07-01
|
Series: | Iranian Journal of Microbiology |
Subjects: | |
Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/2508 |
_version_ | 1798027140124377088 |
---|---|
author | Firas Srhan Abd Al-Mayahi Saja Mahdey Jaber |
author_facet | Firas Srhan Abd Al-Mayahi Saja Mahdey Jaber |
author_sort | Firas Srhan Abd Al-Mayahi |
collection | DOAJ |
description | Background and Objectives: The study was sought to detect the effect of Listeria monocytogenes on pregnant Iraqi women at Al-Diwaniya hospitals and determination of virulence genes and antimicrobial susceptibility of isolates.
Materials and Methods: 360 specimens including blood, urine, vaginal and endocervical were collected from 90 patients with spontaneous abortions. Blood samples were displayed to immunological study and remaining specimens were subjected to bacteriological diagnosis. PCR was used to determine the virulence factors and antimicrobial resistance genes.
Results: Fifteen positive samples (16.6%) of patients and thirteen isolates (14.5%) from patients were recognized based on ELISA and PCR assay respectively. The general isolation of L. monocytogenes strains in cases of abortive women was 13/270 (4.8%). L. monocytogenes strains were highly virulent because of presence of virulence factors associated genes, namely actA, hlyA, plcA and prfA in all strains. Multiple drug resistance (MAR) index values of 15.4% of isolates were >0.2.
Conclusion: It is necessary for conducting susceptibility testing and to select the suitable antibiotics and avoid the effects of these bacteria in pregnant women. |
first_indexed | 2024-04-11T18:47:42Z |
format | Article |
id | doaj.art-b4e4ef1194fb47ad99f04934bbe8517e |
institution | Directory Open Access Journal |
issn | 2008-3289 2008-4447 |
language | English |
last_indexed | 2024-04-11T18:47:42Z |
publishDate | 2020-07-01 |
publisher | Tehran University of Medical Sciences |
record_format | Article |
series | Iranian Journal of Microbiology |
spelling | doaj.art-b4e4ef1194fb47ad99f04934bbe8517e2022-12-22T04:08:42ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472020-07-0112410.18502/ijm.v12i4.3933Multiple drug resistance of Listeria monocytogenes isolated from aborted women by using serological and molecular techniques in Diwaniyah city/ IraqFiras Srhan Abd Al-Mayahi0Saja Mahdey Jaber1Department of Biology, College of Science, University of Al-Qadisiyah, Diwaniyah, IraqDepartment of Science, School of Nursing, University of Al-Qadisiyah, Diwaniyah, IraqBackground and Objectives: The study was sought to detect the effect of Listeria monocytogenes on pregnant Iraqi women at Al-Diwaniya hospitals and determination of virulence genes and antimicrobial susceptibility of isolates. Materials and Methods: 360 specimens including blood, urine, vaginal and endocervical were collected from 90 patients with spontaneous abortions. Blood samples were displayed to immunological study and remaining specimens were subjected to bacteriological diagnosis. PCR was used to determine the virulence factors and antimicrobial resistance genes. Results: Fifteen positive samples (16.6%) of patients and thirteen isolates (14.5%) from patients were recognized based on ELISA and PCR assay respectively. The general isolation of L. monocytogenes strains in cases of abortive women was 13/270 (4.8%). L. monocytogenes strains were highly virulent because of presence of virulence factors associated genes, namely actA, hlyA, plcA and prfA in all strains. Multiple drug resistance (MAR) index values of 15.4% of isolates were >0.2. Conclusion: It is necessary for conducting susceptibility testing and to select the suitable antibiotics and avoid the effects of these bacteria in pregnant women.https://ijm.tums.ac.ir/index.php/ijm/article/view/2508Listeria monocytogenes;Enzyme-linked immunosorbent assay;Polymerase chain reaction;Antibiotic |
spellingShingle | Firas Srhan Abd Al-Mayahi Saja Mahdey Jaber Multiple drug resistance of Listeria monocytogenes isolated from aborted women by using serological and molecular techniques in Diwaniyah city/ Iraq Iranian Journal of Microbiology Listeria monocytogenes; Enzyme-linked immunosorbent assay; Polymerase chain reaction; Antibiotic |
title | Multiple drug resistance of Listeria monocytogenes isolated from aborted women by using serological and molecular techniques in Diwaniyah city/ Iraq |
title_full | Multiple drug resistance of Listeria monocytogenes isolated from aborted women by using serological and molecular techniques in Diwaniyah city/ Iraq |
title_fullStr | Multiple drug resistance of Listeria monocytogenes isolated from aborted women by using serological and molecular techniques in Diwaniyah city/ Iraq |
title_full_unstemmed | Multiple drug resistance of Listeria monocytogenes isolated from aborted women by using serological and molecular techniques in Diwaniyah city/ Iraq |
title_short | Multiple drug resistance of Listeria monocytogenes isolated from aborted women by using serological and molecular techniques in Diwaniyah city/ Iraq |
title_sort | multiple drug resistance of listeria monocytogenes isolated from aborted women by using serological and molecular techniques in diwaniyah city iraq |
topic | Listeria monocytogenes; Enzyme-linked immunosorbent assay; Polymerase chain reaction; Antibiotic |
url | https://ijm.tums.ac.ir/index.php/ijm/article/view/2508 |
work_keys_str_mv | AT firassrhanabdalmayahi multipledrugresistanceoflisteriamonocytogenesisolatedfromabortedwomenbyusingserologicalandmoleculartechniquesindiwaniyahcityiraq AT sajamahdeyjaber multipledrugresistanceoflisteriamonocytogenesisolatedfromabortedwomenbyusingserologicalandmoleculartechniquesindiwaniyahcityiraq |