The role of axonal Kv1 channels in CA3 pyramidal cell excitability
Abstract Axonal ion channels control spike initiation and propagation along the axon and determine action potential waveform. We show here that functional suppression of axonal Kv1 channels with local puff of dendrotoxin (DTx), laser or mechanical axotomy significantly increased excitability measure...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-00388-1 |
| _version_ | 1819039983523069952 |
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| author | Sylvain Rama Mickael Zbili Aurélie Fékété Mónica Tapia Maria José Benitez Norah Boumedine Juan José Garrido Dominique Debanne |
| author_facet | Sylvain Rama Mickael Zbili Aurélie Fékété Mónica Tapia Maria José Benitez Norah Boumedine Juan José Garrido Dominique Debanne |
| author_sort | Sylvain Rama |
| collection | DOAJ |
| description | Abstract Axonal ion channels control spike initiation and propagation along the axon and determine action potential waveform. We show here that functional suppression of axonal Kv1 channels with local puff of dendrotoxin (DTx), laser or mechanical axotomy significantly increased excitability measured in the cell body. Importantly, the functional effect of DTx puffing or axotomy was not limited to the axon initial segment but was also seen on axon collaterals. In contrast, no effects were observed when DTx was puffed on single apical dendrites or after single dendrotomy. A simple model with Kv1 located in the axon reproduced the experimental observations and showed that the distance at which the effects of axon collateral cuts are seen depends on the axon space constant. In conclusion, Kv1 channels located in the axon proper greatly participate in intrinsic excitability of CA3 pyramidal neurons. This finding stresses the importance of the axonal compartment in the regulation of intrinsic neuronal excitability. |
| first_indexed | 2024-12-21T09:01:52Z |
| format | Article |
| id | doaj.art-b4e80117a02f4e5d86fe9015fa2624b5 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-21T09:01:52Z |
| publishDate | 2017-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b4e80117a02f4e5d86fe9015fa2624b52022-12-21T19:09:28ZengNature PortfolioScientific Reports2045-23222017-03-017111210.1038/s41598-017-00388-1The role of axonal Kv1 channels in CA3 pyramidal cell excitabilitySylvain Rama0Mickael Zbili1Aurélie Fékété2Mónica Tapia3Maria José Benitez4Norah Boumedine5Juan José Garrido6Dominique Debanne7UNIS, INSERM UMR_S 1072, Aix-Marseille UniversitéUNIS, INSERM UMR_S 1072, Aix-Marseille UniversitéUNIS, INSERM UMR_S 1072, Aix-Marseille UniversitéInstituto Cajal, CSICInstituto Cajal, CSICUNIS, INSERM UMR_S 1072, Aix-Marseille UniversitéInstituto Cajal, CSICUNIS, INSERM UMR_S 1072, Aix-Marseille UniversitéAbstract Axonal ion channels control spike initiation and propagation along the axon and determine action potential waveform. We show here that functional suppression of axonal Kv1 channels with local puff of dendrotoxin (DTx), laser or mechanical axotomy significantly increased excitability measured in the cell body. Importantly, the functional effect of DTx puffing or axotomy was not limited to the axon initial segment but was also seen on axon collaterals. In contrast, no effects were observed when DTx was puffed on single apical dendrites or after single dendrotomy. A simple model with Kv1 located in the axon reproduced the experimental observations and showed that the distance at which the effects of axon collateral cuts are seen depends on the axon space constant. In conclusion, Kv1 channels located in the axon proper greatly participate in intrinsic excitability of CA3 pyramidal neurons. This finding stresses the importance of the axonal compartment in the regulation of intrinsic neuronal excitability.https://doi.org/10.1038/s41598-017-00388-1 |
| spellingShingle | Sylvain Rama Mickael Zbili Aurélie Fékété Mónica Tapia Maria José Benitez Norah Boumedine Juan José Garrido Dominique Debanne The role of axonal Kv1 channels in CA3 pyramidal cell excitability Scientific Reports |
| title | The role of axonal Kv1 channels in CA3 pyramidal cell excitability |
| title_full | The role of axonal Kv1 channels in CA3 pyramidal cell excitability |
| title_fullStr | The role of axonal Kv1 channels in CA3 pyramidal cell excitability |
| title_full_unstemmed | The role of axonal Kv1 channels in CA3 pyramidal cell excitability |
| title_short | The role of axonal Kv1 channels in CA3 pyramidal cell excitability |
| title_sort | role of axonal kv1 channels in ca3 pyramidal cell excitability |
| url | https://doi.org/10.1038/s41598-017-00388-1 |
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