Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2
Rett syndrome (RTT) is a childhood neurodevelopmental disorder caused by mutations in MECP2. To study the molecular mechanisms underlying RTT, four sublines of H1 hESCs were generated, carrying a hemizygous knockout or mutant allele of MECP2. Exons 3 and 4 of MECP2 were targeted using the CRISPR/Cas...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-10-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506119301631 |
| _version_ | 1818061694542282752 |
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| author | Ruizhu Zeng Harwin Sidik Kim S. Robinson Franklin L. Zhong Bruno Reversade Mahmoud A. Pouladi |
| author_facet | Ruizhu Zeng Harwin Sidik Kim S. Robinson Franklin L. Zhong Bruno Reversade Mahmoud A. Pouladi |
| author_sort | Ruizhu Zeng |
| collection | DOAJ |
| description | Rett syndrome (RTT) is a childhood neurodevelopmental disorder caused by mutations in MECP2. To study the molecular mechanisms underlying RTT, four sublines of H1 hESCs were generated, carrying a hemizygous knockout or mutant allele of MECP2. Exons 3 and 4 of MECP2 were targeted using the CRISPR/Cas9 nuclease system. |
| first_indexed | 2024-12-10T13:52:23Z |
| format | Article |
| id | doaj.art-b4e964aa11f34855b2d9d264bddf4dbf |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-10T13:52:23Z |
| publishDate | 2019-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-b4e964aa11f34855b2d9d264bddf4dbf2022-12-22T01:46:07ZengElsevierStem Cell Research1873-50612019-10-0140Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2Ruizhu Zeng0Harwin Sidik1Kim S. Robinson2Franklin L. Zhong3Bruno Reversade4Mahmoud A. Pouladi5Translational Laboratory in Genetic Medicine, Agency for Science, Technology and Research, Singapore (A*STAR), 8A Biomedical Grove, Immunos, Level 5, 138648, SingaporeTranslational Laboratory in Genetic Medicine, Agency for Science, Technology and Research, Singapore (A*STAR), 8A Biomedical Grove, Immunos, Level 5, 138648, SingaporeInstitute of Medical Biology, A*STAR, Immunos, 138648, Singapore; Skin Research Institute of Singapore, Immunos, 138648, SingaporeInstitute of Molecular and Cell Biology, A*STAR, Proteos, 138673, Singapore.; Institute of Medical Biology, A*STAR, Immunos, 138648, Singapore; Skin Research Institute of Singapore, Immunos, 138648, Singapore; Lee Kong Chian School of Medicine, Nanyang Technological University, 636921, SingaporeInstitute of Molecular and Cell Biology, A*STAR, Proteos, 138673, Singapore.; Institute of Medical Biology, A*STAR, Immunos, 138648, Singapore; Department of Paediatrics, National University of Singapore, 1E Kent Ridge Road, 119228, Singapore; Medical Genetics Department, Koç University School of Medicine, 34010 Istanbul, TurkeyTranslational Laboratory in Genetic Medicine, Agency for Science, Technology and Research, Singapore (A*STAR), 8A Biomedical Grove, Immunos, Level 5, 138648, Singapore; Department of Medicine, National University of Singapore, 117597, Singapore; Department of Physiology, National University of Singapore, 117597, Singapore; Corresponding author at: Translational Laboratory in Genetic Medicine, Agency for Science, Technology and Research, Singapore (A*STAR), 8A Biomedical Grove, Immunos, Level 5, 138648, Singapore.Rett syndrome (RTT) is a childhood neurodevelopmental disorder caused by mutations in MECP2. To study the molecular mechanisms underlying RTT, four sublines of H1 hESCs were generated, carrying a hemizygous knockout or mutant allele of MECP2. Exons 3 and 4 of MECP2 were targeted using the CRISPR/Cas9 nuclease system.http://www.sciencedirect.com/science/article/pii/S1873506119301631 |
| spellingShingle | Ruizhu Zeng Harwin Sidik Kim S. Robinson Franklin L. Zhong Bruno Reversade Mahmoud A. Pouladi Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2 Stem Cell Research |
| title | Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2 |
| title_full | Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2 |
| title_fullStr | Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2 |
| title_full_unstemmed | Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2 |
| title_short | Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2 |
| title_sort | generation of four h1 hesc sublines carrying a hemizygous knock out mutant mecp2 |
| url | http://www.sciencedirect.com/science/article/pii/S1873506119301631 |
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