Transcriptional Analysis of C-Repeat Binding Factors in Fruit of <i>Citrus</i> Species with Differential Sensitivity to Chilling Injury during Postharvest Storage
Citrus fruit are sensitive to chilling injury (CI) during cold storage, a peel disorder that causes economic losses. C-repeat binding factors (CBFs) are related to cold acclimation and tolerance in different plants. To explore the role of <i>Citrus</i> CBFs in fruit response to cold, an...
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/2/804 |
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| author | Matías Salvo Florencia Rey Ana Arruabarrena Giuliana Gambetta María J. Rodrigo Lorenzo Zacarías Joanna Lado |
| author_facet | Matías Salvo Florencia Rey Ana Arruabarrena Giuliana Gambetta María J. Rodrigo Lorenzo Zacarías Joanna Lado |
| author_sort | Matías Salvo |
| collection | DOAJ |
| description | Citrus fruit are sensitive to chilling injury (CI) during cold storage, a peel disorder that causes economic losses. C-repeat binding factors (CBFs) are related to cold acclimation and tolerance in different plants. To explore the role of <i>Citrus</i> CBFs in fruit response to cold, an in silico study was performed, revealing three genes (<i>CBF1</i>, <i>CBF2</i>, and <i>CBF3</i>) whose expression in CI sensitive and tolerant cultivars was followed. Major changes occurred at the early stages of cold exposure (1–5 d). Interestingly, <i>CBF1</i> was the most stimulated gene in the peel of CI-tolerant cultivars (Lisbon lemon, Star Ruby grapefruit, and Navelina orange), remaining unaltered in sensitive cultivars (Meyer lemon, Marsh grapefruit, and Salustiana orange). Results suggest a positive association of <i>CBF1</i> expression with cold tolerance in <i>Citrus</i> cultivars (except for mandarins), whereas the expression of <i>CBF2</i> or <i>CBF3</i> genes did not reveal a clear relationship with the susceptibility to CI. Light avoidance during fruit growth reduced postharvest CI in most sensitive cultivars, associated with a rapid and transient enhance in the expression of the three CBFs. Results suggest that CBFs-dependent pathways mediate at least part of the cold tolerance responses in sensitive <i>Citrus</i>, indicating that <i>CBF1</i> participates in the natural tolerance to CI. |
| first_indexed | 2024-03-09T04:44:14Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T04:44:14Z |
| publishDate | 2021-01-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b4ebef87cc5e4e63ad8ba63f9fd9f68e2023-12-03T13:17:59ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-0122280410.3390/ijms22020804Transcriptional Analysis of C-Repeat Binding Factors in Fruit of <i>Citrus</i> Species with Differential Sensitivity to Chilling Injury during Postharvest StorageMatías Salvo0Florencia Rey1Ana Arruabarrena2Giuliana Gambetta3María J. Rodrigo4Lorenzo Zacarías5Joanna Lado6Programa de Investigación en Citricultura, Estación Experimental INIA Salto Grande, Instituto Nacional de Investigación Agropecuaria (INIA), Camino a la Represa s/n, 50000 Salto, UruguayFood Biotechnology Department, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Paterna, 46980 Valencia, SpainPrograma de Investigación en Citricultura, Estación Experimental INIA Salto Grande, Instituto Nacional de Investigación Agropecuaria (INIA), Camino a la Represa s/n, 50000 Salto, UruguayDepartamento de Producción Vegetal, Facultad de Agronomía, Universidad de la República, Garzón 780, 11900 Montevideo, UruguayFood Biotechnology Department, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Paterna, 46980 Valencia, SpainFood Biotechnology Department, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Paterna, 46980 Valencia, SpainPrograma de Investigación en Citricultura, Estación Experimental INIA Salto Grande, Instituto Nacional de Investigación Agropecuaria (INIA), Camino a la Represa s/n, 50000 Salto, UruguayCitrus fruit are sensitive to chilling injury (CI) during cold storage, a peel disorder that causes economic losses. C-repeat binding factors (CBFs) are related to cold acclimation and tolerance in different plants. To explore the role of <i>Citrus</i> CBFs in fruit response to cold, an in silico study was performed, revealing three genes (<i>CBF1</i>, <i>CBF2</i>, and <i>CBF3</i>) whose expression in CI sensitive and tolerant cultivars was followed. Major changes occurred at the early stages of cold exposure (1–5 d). Interestingly, <i>CBF1</i> was the most stimulated gene in the peel of CI-tolerant cultivars (Lisbon lemon, Star Ruby grapefruit, and Navelina orange), remaining unaltered in sensitive cultivars (Meyer lemon, Marsh grapefruit, and Salustiana orange). Results suggest a positive association of <i>CBF1</i> expression with cold tolerance in <i>Citrus</i> cultivars (except for mandarins), whereas the expression of <i>CBF2</i> or <i>CBF3</i> genes did not reveal a clear relationship with the susceptibility to CI. Light avoidance during fruit growth reduced postharvest CI in most sensitive cultivars, associated with a rapid and transient enhance in the expression of the three CBFs. Results suggest that CBFs-dependent pathways mediate at least part of the cold tolerance responses in sensitive <i>Citrus</i>, indicating that <i>CBF1</i> participates in the natural tolerance to CI.https://www.mdpi.com/1422-0067/22/2/804CBFcitruschilling injuryDREBgene expression |
| spellingShingle | Matías Salvo Florencia Rey Ana Arruabarrena Giuliana Gambetta María J. Rodrigo Lorenzo Zacarías Joanna Lado Transcriptional Analysis of C-Repeat Binding Factors in Fruit of <i>Citrus</i> Species with Differential Sensitivity to Chilling Injury during Postharvest Storage International Journal of Molecular Sciences CBF citrus chilling injury DREB gene expression |
| title | Transcriptional Analysis of C-Repeat Binding Factors in Fruit of <i>Citrus</i> Species with Differential Sensitivity to Chilling Injury during Postharvest Storage |
| title_full | Transcriptional Analysis of C-Repeat Binding Factors in Fruit of <i>Citrus</i> Species with Differential Sensitivity to Chilling Injury during Postharvest Storage |
| title_fullStr | Transcriptional Analysis of C-Repeat Binding Factors in Fruit of <i>Citrus</i> Species with Differential Sensitivity to Chilling Injury during Postharvest Storage |
| title_full_unstemmed | Transcriptional Analysis of C-Repeat Binding Factors in Fruit of <i>Citrus</i> Species with Differential Sensitivity to Chilling Injury during Postharvest Storage |
| title_short | Transcriptional Analysis of C-Repeat Binding Factors in Fruit of <i>Citrus</i> Species with Differential Sensitivity to Chilling Injury during Postharvest Storage |
| title_sort | transcriptional analysis of c repeat binding factors in fruit of i citrus i species with differential sensitivity to chilling injury during postharvest storage |
| topic | CBF citrus chilling injury DREB gene expression |
| url | https://www.mdpi.com/1422-0067/22/2/804 |
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