THE TECHNIQUE OF FLOW CYTOMETRY IN DIAGNOSTIC RESEARCH
Flow cytometry is a technology that simultaneously counts and then examines multiple physical and/or chemical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity o...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Lublin University of Technology
2013-09-01
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Series: | Advances in Sciences and Technology |
Subjects: | |
Online Access: | http://www.journalssystem.com/astrj/The-technique-of-flow-cytometry-in-diagnostic-research,185,0,2.html |
Summary: | Flow cytometry is a technology that simultaneously counts and then examines multiple physical and/or chemical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity or internal complexity, and relative fluorescence intensity. These characteristics are determined using an optical-to-electronic coupling system that records how the cell scatters incident laser light and emits fluorescence. One of the most significant applications is immunophenotyping of cells - the most important tool in diagnosis and monitoring haematological disorders, such as acute and chronic leukemia, lymphoma, monoclonal gammopathy, myelodisplastic and myeloproliferative diseases. |
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ISSN: | 2080-4075 2299-8624 |