THE TECHNIQUE OF FLOW CYTOMETRY IN DIAGNOSTIC RESEARCH

Flow cytometry is a technology that simultaneously counts and then examines multiple physical and/or chemical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity o...

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Main Authors: Arkadiusz Macheta, Katarzyna Radko, Agnieszka Szymczyk, Monika Podhorecka
Format: Article
Language:English
Published: Lublin University of Technology 2013-09-01
Series:Advances in Sciences and Technology
Subjects:
Online Access:http://www.journalssystem.com/astrj/The-technique-of-flow-cytometry-in-diagnostic-research,185,0,2.html
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author Arkadiusz Macheta
Katarzyna Radko
Agnieszka Szymczyk
Monika Podhorecka
author_facet Arkadiusz Macheta
Katarzyna Radko
Agnieszka Szymczyk
Monika Podhorecka
author_sort Arkadiusz Macheta
collection DOAJ
description Flow cytometry is a technology that simultaneously counts and then examines multiple physical and/or chemical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity or internal complexity, and relative fluorescence intensity. These characteristics are determined using an optical-to-electronic coupling system that records how the cell scatters incident laser light and emits fluorescence. One of the most significant applications is immunophenotyping of cells - the most important tool in diagnosis and monitoring haematological disorders, such as acute and chronic leukemia, lymphoma, monoclonal gammopathy, myelodisplastic and myeloproliferative diseases.
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spelling doaj.art-b4ec635f595d4b329fa48e5edb5eb1432022-12-22T02:43:07ZengLublin University of TechnologyAdvances in Sciences and Technology2080-40752299-86242013-09-01719555910.5604/20804075.1062364185THE TECHNIQUE OF FLOW CYTOMETRY IN DIAGNOSTIC RESEARCHArkadiusz Macheta0Katarzyna Radko1Agnieszka Szymczyk2Monika Podhorecka3Department of Hematooncology and Bone Marrow Transplantation, Medical University of Lublin, Al. Racławickie 1, 20-059 Lublin, PolandDepartment of Hematooncology and Bone Marrow Transplantation, Medical University of Lublin, Al. Racławickie 1, 20-059 Lublin, PolandStudents Scientific Association at Department of Hematooncology and Bone Marrow Transplantation Medical University of Lublin, Al. Racławickie 1, 20-059 Lublin, PolandDepartment of Hematooncology and Bone Marrow Transplantation, Medical University of Lublin, Al. Racławickie 1, 20-059 Lublin, PolandFlow cytometry is a technology that simultaneously counts and then examines multiple physical and/or chemical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity or internal complexity, and relative fluorescence intensity. These characteristics are determined using an optical-to-electronic coupling system that records how the cell scatters incident laser light and emits fluorescence. One of the most significant applications is immunophenotyping of cells - the most important tool in diagnosis and monitoring haematological disorders, such as acute and chronic leukemia, lymphoma, monoclonal gammopathy, myelodisplastic and myeloproliferative diseases.http://www.journalssystem.com/astrj/The-technique-of-flow-cytometry-in-diagnostic-research,185,0,2.htmlflow cytometryimmunophenotypehematooncology
spellingShingle Arkadiusz Macheta
Katarzyna Radko
Agnieszka Szymczyk
Monika Podhorecka
THE TECHNIQUE OF FLOW CYTOMETRY IN DIAGNOSTIC RESEARCH
Advances in Sciences and Technology
flow cytometry
immunophenotype
hematooncology
title THE TECHNIQUE OF FLOW CYTOMETRY IN DIAGNOSTIC RESEARCH
title_full THE TECHNIQUE OF FLOW CYTOMETRY IN DIAGNOSTIC RESEARCH
title_fullStr THE TECHNIQUE OF FLOW CYTOMETRY IN DIAGNOSTIC RESEARCH
title_full_unstemmed THE TECHNIQUE OF FLOW CYTOMETRY IN DIAGNOSTIC RESEARCH
title_short THE TECHNIQUE OF FLOW CYTOMETRY IN DIAGNOSTIC RESEARCH
title_sort technique of flow cytometry in diagnostic research
topic flow cytometry
immunophenotype
hematooncology
url http://www.journalssystem.com/astrj/The-technique-of-flow-cytometry-in-diagnostic-research,185,0,2.html
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