NRAMP1 gene polymorphisms and cutaneous leishmaniasis: An evaluation on host susceptibility and treatment outcome
Background & objectives: Association between polymorphisms in the natural resistance associated macrophage protein 1 (NRAMP1) gene and susceptibility to cutaneous leishmaniasis (CL) has been demonstrated worldwide; however, the reported results were inconsistent. This study aimed to determine th...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2016-01-01
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| Series: | Journal of Vector Borne Diseases |
| Subjects: | |
| Online Access: | http://www.jvbd.org/article.asp?issn=0972-9062;year=2016;volume=53;issue=3;spage=257;epage=263;aulast=Fattahi-Dolatabadi |
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| author | Marzieh Fattahi-Dolatabadi Tahereh Mousavi Hajar Mohammadi-Barzelighi Saeed Irian Bita Bakhshi Mohammad-Ali Nilforoushzadeh Leila Shirani-Bidabadi Mohammad-Mahdi Hariri Nazli Ansari Nahid Akbari |
| author_facet | Marzieh Fattahi-Dolatabadi Tahereh Mousavi Hajar Mohammadi-Barzelighi Saeed Irian Bita Bakhshi Mohammad-Ali Nilforoushzadeh Leila Shirani-Bidabadi Mohammad-Mahdi Hariri Nazli Ansari Nahid Akbari |
| author_sort | Marzieh Fattahi-Dolatabadi |
| collection | DOAJ |
| description | Background & objectives: Association between polymorphisms in the natural resistance associated macrophage protein 1 (NRAMP1) gene and susceptibility to cutaneous leishmaniasis (CL) has been demonstrated worldwide; however, the reported results were inconsistent. This study aimed to determine the association of NRAMP1 variants with susceptibility to CL infection and patients′ response to treatment in Isfahan province of Iran.
Methods: Peripheral blood samples were collected from 150 patients with CL and 136 healthy controls. The CL patients were treated with intralesional injection of meglumine antimoniate. The polymorphic variants at NRAMP1 (A318V and D543N) were analyzed using PCR-RFLP. The chi-square test and Fisher′s exact test were used to compare frequencies of alleles and genotypes of polymorphisms between patient and healthy control populations.
Results: There was a statistically significant difference in the D543N (rs17235409) polymorphism between the CL patients and healthy controls (p=0.008). However, no significant association was detected for A318V (rs201565523) polymorphism between groups (p=0.26). In addition, there was a lack of association between D543N and A318V genotypes with response to treatment (p=0.54 and p=0.31, respectively).
Interpretation & conclusion: The results indicated that genetic variations of D543N (rs17235409) might be associated with susceptibility to CL infection. These data may be used for detection of sensitive individuals and prevention of CL in endemic areas. |
| first_indexed | 2024-12-11T06:14:47Z |
| format | Article |
| id | doaj.art-b4ec9f06e2684914a987363bef6480fd |
| institution | Directory Open Access Journal |
| issn | 0972-9062 |
| language | English |
| last_indexed | 2024-12-11T06:14:47Z |
| publishDate | 2016-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Vector Borne Diseases |
| spelling | doaj.art-b4ec9f06e2684914a987363bef6480fd2022-12-22T01:18:00ZengWolters Kluwer Medknow PublicationsJournal of Vector Borne Diseases0972-90622016-01-01533257263NRAMP1 gene polymorphisms and cutaneous leishmaniasis: An evaluation on host susceptibility and treatment outcomeMarzieh Fattahi-DolatabadiTahereh MousaviHajar Mohammadi-BarzelighiSaeed IrianBita BakhshiMohammad-Ali NilforoushzadehLeila Shirani-BidabadiMohammad-Mahdi HaririNazli AnsariNahid AkbariBackground & objectives: Association between polymorphisms in the natural resistance associated macrophage protein 1 (NRAMP1) gene and susceptibility to cutaneous leishmaniasis (CL) has been demonstrated worldwide; however, the reported results were inconsistent. This study aimed to determine the association of NRAMP1 variants with susceptibility to CL infection and patients′ response to treatment in Isfahan province of Iran. Methods: Peripheral blood samples were collected from 150 patients with CL and 136 healthy controls. The CL patients were treated with intralesional injection of meglumine antimoniate. The polymorphic variants at NRAMP1 (A318V and D543N) were analyzed using PCR-RFLP. The chi-square test and Fisher′s exact test were used to compare frequencies of alleles and genotypes of polymorphisms between patient and healthy control populations. Results: There was a statistically significant difference in the D543N (rs17235409) polymorphism between the CL patients and healthy controls (p=0.008). However, no significant association was detected for A318V (rs201565523) polymorphism between groups (p=0.26). In addition, there was a lack of association between D543N and A318V genotypes with response to treatment (p=0.54 and p=0.31, respectively). Interpretation & conclusion: The results indicated that genetic variations of D543N (rs17235409) might be associated with susceptibility to CL infection. These data may be used for detection of sensitive individuals and prevention of CL in endemic areas.http://www.jvbd.org/article.asp?issn=0972-9062;year=2016;volume=53;issue=3;spage=257;epage=263;aulast=Fattahi-DolatabadiA318Vcutaneous D543NIranleishmaniasisNRAMP1 |
| spellingShingle | Marzieh Fattahi-Dolatabadi Tahereh Mousavi Hajar Mohammadi-Barzelighi Saeed Irian Bita Bakhshi Mohammad-Ali Nilforoushzadeh Leila Shirani-Bidabadi Mohammad-Mahdi Hariri Nazli Ansari Nahid Akbari NRAMP1 gene polymorphisms and cutaneous leishmaniasis: An evaluation on host susceptibility and treatment outcome Journal of Vector Borne Diseases A318V cutaneous D543N Iran leishmaniasis NRAMP1 |
| title | NRAMP1 gene polymorphisms and cutaneous leishmaniasis: An evaluation on host susceptibility and treatment outcome |
| title_full | NRAMP1 gene polymorphisms and cutaneous leishmaniasis: An evaluation on host susceptibility and treatment outcome |
| title_fullStr | NRAMP1 gene polymorphisms and cutaneous leishmaniasis: An evaluation on host susceptibility and treatment outcome |
| title_full_unstemmed | NRAMP1 gene polymorphisms and cutaneous leishmaniasis: An evaluation on host susceptibility and treatment outcome |
| title_short | NRAMP1 gene polymorphisms and cutaneous leishmaniasis: An evaluation on host susceptibility and treatment outcome |
| title_sort | nramp1 gene polymorphisms and cutaneous leishmaniasis an evaluation on host susceptibility and treatment outcome |
| topic | A318V cutaneous D543N Iran leishmaniasis NRAMP1 |
| url | http://www.jvbd.org/article.asp?issn=0972-9062;year=2016;volume=53;issue=3;spage=257;epage=263;aulast=Fattahi-Dolatabadi |
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