Oral exposure to Staphylococcus aureus enterotoxin B could promote the Ovalbumin-induced food allergy by enhancing the activation of DCs and T cells
IntroductionRecent work highlighted the importance of environmental contaminants in the development of allergic diseases.MethodsThe intestinal mucosal barrier, Th (helper T) cells, DCs (dendritic cells), and intestinal flora were analyzed with flow cytometry, RNA-seq, and 16s sequencing in the prese...
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1250458/full |
| _version_ | 1827792947014270976 |
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| author | Jin Yuan Jin Yuan Jin Yuan Ping Tong Xuanyi Meng Yong Wu Xin Li Xin Li Jinyan Gao Hongbing Chen Hongbing Chen |
| author_facet | Jin Yuan Jin Yuan Jin Yuan Ping Tong Xuanyi Meng Yong Wu Xin Li Xin Li Jinyan Gao Hongbing Chen Hongbing Chen |
| author_sort | Jin Yuan |
| collection | DOAJ |
| description | IntroductionRecent work highlighted the importance of environmental contaminants in the development of allergic diseases.MethodsThe intestinal mucosal barrier, Th (helper T) cells, DCs (dendritic cells), and intestinal flora were analyzed with flow cytometry, RNA-seq, and 16s sequencing in the present study to demonstrate whether the exposure of enterotoxins like Staphylococcus aureus enterotoxin B (SEB) in allergens could promote the development of food allergy.Results and discussionWe found that co-exposure to SEB and Ovalbumin (OVA) could impair the intestinal barrier, imbalance the intestinal Th immune, and cause the decline of intestinal flora diversity in OVA-sensitized mice. Moreover, with the co-stimulation of SEB, the transport of OVA was enhanced in the Caco-2 cell monolayer, the uptake and presentation of OVA were promoted in the bone marrow dendritic cells (BMDCs), and Th cell differentiation was also enhanced. In summary, co-exposure to SEB in allergens should be considered a food allergy risk factor. |
| first_indexed | 2024-03-11T18:10:27Z |
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| id | doaj.art-b4ed5b535daf429b8b8cf8916c88b12d |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-11T18:10:27Z |
| publishDate | 2023-10-01 |
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| series | Frontiers in Immunology |
| spelling | doaj.art-b4ed5b535daf429b8b8cf8916c88b12d2023-10-16T15:30:27ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-10-011410.3389/fimmu.2023.12504581250458Oral exposure to Staphylococcus aureus enterotoxin B could promote the Ovalbumin-induced food allergy by enhancing the activation of DCs and T cellsJin Yuan0Jin Yuan1Jin Yuan2Ping Tong3Xuanyi Meng4Yong Wu5Xin Li6Xin Li7Jinyan Gao8Hongbing Chen9Hongbing Chen10State Key Laboratory of Food Science and Resource, Nanchang University, Nanchang, ChinaSino-German Joint Research Institute, Nanchang University, Nanchang, ChinaCollege of Food Science & Technology, Nanchang University, Nanchang, ChinaState Key Laboratory of Food Science and Resource, Nanchang University, Nanchang, ChinaSino-German Joint Research Institute, Nanchang University, Nanchang, ChinaSino-German Joint Research Institute, Nanchang University, Nanchang, ChinaState Key Laboratory of Food Science and Resource, Nanchang University, Nanchang, ChinaCollege of Food Science & Technology, Nanchang University, Nanchang, ChinaCollege of Food Science & Technology, Nanchang University, Nanchang, ChinaState Key Laboratory of Food Science and Resource, Nanchang University, Nanchang, ChinaSino-German Joint Research Institute, Nanchang University, Nanchang, ChinaIntroductionRecent work highlighted the importance of environmental contaminants in the development of allergic diseases.MethodsThe intestinal mucosal barrier, Th (helper T) cells, DCs (dendritic cells), and intestinal flora were analyzed with flow cytometry, RNA-seq, and 16s sequencing in the present study to demonstrate whether the exposure of enterotoxins like Staphylococcus aureus enterotoxin B (SEB) in allergens could promote the development of food allergy.Results and discussionWe found that co-exposure to SEB and Ovalbumin (OVA) could impair the intestinal barrier, imbalance the intestinal Th immune, and cause the decline of intestinal flora diversity in OVA-sensitized mice. Moreover, with the co-stimulation of SEB, the transport of OVA was enhanced in the Caco-2 cell monolayer, the uptake and presentation of OVA were promoted in the bone marrow dendritic cells (BMDCs), and Th cell differentiation was also enhanced. In summary, co-exposure to SEB in allergens should be considered a food allergy risk factor.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1250458/fullStaphylococcus aureus enterotoxin Bfood allergydendritic cellsT cellsgut microbiota |
| spellingShingle | Jin Yuan Jin Yuan Jin Yuan Ping Tong Xuanyi Meng Yong Wu Xin Li Xin Li Jinyan Gao Hongbing Chen Hongbing Chen Oral exposure to Staphylococcus aureus enterotoxin B could promote the Ovalbumin-induced food allergy by enhancing the activation of DCs and T cells Frontiers in Immunology Staphylococcus aureus enterotoxin B food allergy dendritic cells T cells gut microbiota |
| title | Oral exposure to Staphylococcus aureus enterotoxin B could promote the Ovalbumin-induced food allergy by enhancing the activation of DCs and T cells |
| title_full | Oral exposure to Staphylococcus aureus enterotoxin B could promote the Ovalbumin-induced food allergy by enhancing the activation of DCs and T cells |
| title_fullStr | Oral exposure to Staphylococcus aureus enterotoxin B could promote the Ovalbumin-induced food allergy by enhancing the activation of DCs and T cells |
| title_full_unstemmed | Oral exposure to Staphylococcus aureus enterotoxin B could promote the Ovalbumin-induced food allergy by enhancing the activation of DCs and T cells |
| title_short | Oral exposure to Staphylococcus aureus enterotoxin B could promote the Ovalbumin-induced food allergy by enhancing the activation of DCs and T cells |
| title_sort | oral exposure to staphylococcus aureus enterotoxin b could promote the ovalbumin induced food allergy by enhancing the activation of dcs and t cells |
| topic | Staphylococcus aureus enterotoxin B food allergy dendritic cells T cells gut microbiota |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1250458/full |
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