Development and Validation of RP-HPLC Method for the Determination of Dexrabeprazole Sodium
A rapid and simple liquid chromatographic method was developed for the quantitative determination of dexrabeprazole sodium in the tablet dosage form. The reverse-phase chromatographic analysis was carried out by using a C-18 column. The acetonitrile-phosphate buffer mixture was used as the mobile ph...
| Main Authors: | , , |
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| 格式: | Article |
| 語言: | English |
| 出版: |
Sakarya University
2021-12-01
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| 叢編: | Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi |
| 主題: | |
| 在線閱讀: | https://dergipark.org.tr/tr/download/article-file/1989694 |
| _version_ | 1826923603508068352 |
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| author | Semra Yılmazer Keskin Cihansel Unlu Ebru Nurdan Şentürk |
| author_facet | Semra Yılmazer Keskin Cihansel Unlu Ebru Nurdan Şentürk |
| author_sort | Semra Yılmazer Keskin |
| collection | DOAJ |
| description | A rapid and simple liquid chromatographic method was developed for the quantitative determination of dexrabeprazole sodium in the tablet dosage form. The reverse-phase chromatographic analysis was carried out by using a C-18 column. The acetonitrile-phosphate buffer mixture was used as the mobile phase. The obtained retention time was 4.33∓0.02 min. by using 50:50 (v:v) acetonitrile:phosphate buffer(pH 7) and 1.0 mL/min. flow rate. Quantification of the analyte was based on measuring the peaks areas at 272 nm. The analytical performance of the developed RP-HPLC method was validated with respect to accuracy, precision, linearity, stability, and robustness. The obtained linearity range was 77-143 mg/L and the correlation coefficient was 0.9989. The obtained LOD and LOQ values were 0.010 mg/L and 0.034 mg/L, respectively. |
| first_indexed | 2025-02-17T14:28:27Z |
| format | Article |
| id | doaj.art-b4ee1c128e344716b169b51cf7390cbf |
| institution | Directory Open Access Journal |
| issn | 2147-835X |
| language | English |
| last_indexed | 2025-02-17T14:28:27Z |
| publishDate | 2021-12-01 |
| publisher | Sakarya University |
| record_format | Article |
| series | Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi |
| spelling | doaj.art-b4ee1c128e344716b169b51cf7390cbf2024-12-23T08:08:56ZengSakarya UniversitySakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi2147-835X2021-12-012561427143110.16984/saufenbilder.99945828Development and Validation of RP-HPLC Method for the Determination of Dexrabeprazole SodiumSemra Yılmazer Keskin0https://orcid.org/0000-0002-9467-3171Cihansel Unlu1https://orcid.org/0000-0001-5676-4323Ebru Nurdan Şentürk2https://orcid.org/0000-0003-1496-4284SAKARYA ÜNİVERSİTESİSAKARYA ÜNİVERSİTESİSAKARYA ÜNİVERSİTESİA rapid and simple liquid chromatographic method was developed for the quantitative determination of dexrabeprazole sodium in the tablet dosage form. The reverse-phase chromatographic analysis was carried out by using a C-18 column. The acetonitrile-phosphate buffer mixture was used as the mobile phase. The obtained retention time was 4.33∓0.02 min. by using 50:50 (v:v) acetonitrile:phosphate buffer(pH 7) and 1.0 mL/min. flow rate. Quantification of the analyte was based on measuring the peaks areas at 272 nm. The analytical performance of the developed RP-HPLC method was validated with respect to accuracy, precision, linearity, stability, and robustness. The obtained linearity range was 77-143 mg/L and the correlation coefficient was 0.9989. The obtained LOD and LOQ values were 0.010 mg/L and 0.034 mg/L, respectively.https://dergipark.org.tr/tr/download/article-file/1989694dexrabeprazolerp-hplcvalidation |
| spellingShingle | Semra Yılmazer Keskin Cihansel Unlu Ebru Nurdan Şentürk Development and Validation of RP-HPLC Method for the Determination of Dexrabeprazole Sodium Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi dexrabeprazole rp-hplc validation |
| title | Development and Validation of RP-HPLC Method for the Determination of Dexrabeprazole Sodium |
| title_full | Development and Validation of RP-HPLC Method for the Determination of Dexrabeprazole Sodium |
| title_fullStr | Development and Validation of RP-HPLC Method for the Determination of Dexrabeprazole Sodium |
| title_full_unstemmed | Development and Validation of RP-HPLC Method for the Determination of Dexrabeprazole Sodium |
| title_short | Development and Validation of RP-HPLC Method for the Determination of Dexrabeprazole Sodium |
| title_sort | development and validation of rp hplc method for the determination of dexrabeprazole sodium |
| topic | dexrabeprazole rp-hplc validation |
| url | https://dergipark.org.tr/tr/download/article-file/1989694 |
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