Protective effect of bone marrow mesenchymal stem cells modified with klotho on renal ischemia-reperfusion injury
Objective: To detect the combination protective effect of bone marrow mesenchymal stem cells (BMSCs) and Klotho gene on the renal ischemia-reperfusion injury (RIRI). Methods: BMSCs isolated from rats were transfected with Klotho gene to form BMSCKl. We injected BMSCKl to allogenic rat RIRI model. Af...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Renal Failure |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/0886022X.2019.1588131 |
| _version_ | 1819101837419085824 |
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| author | Li-Bo Xie Xi Chen Bo Chen Xian-Ding Wang Rui Jiang Yi-Ping Lu |
| author_facet | Li-Bo Xie Xi Chen Bo Chen Xian-Ding Wang Rui Jiang Yi-Ping Lu |
| author_sort | Li-Bo Xie |
| collection | DOAJ |
| description | Objective: To detect the combination protective effect of bone marrow mesenchymal stem cells (BMSCs) and Klotho gene on the renal ischemia-reperfusion injury (RIRI). Methods: BMSCs isolated from rats were transfected with Klotho gene to form BMSCKl. We injected BMSCKl to allogenic rat RIRI model. After 24 h and 72 h, we detected the serum creatinine (SCr), malondialdehyde (MDA), and superoxide dismutase (SOD) in renal tissue, Hematoxylin-eosin (HE) staining, and TUNEL of renal pathology. The expression of FoxO1 and p-FoxO1 in post-hypoxia tubular epithelial cells of normal rat kidney (NRK-52E) were detected by Western blot after cocultured with BMSCKl. Results: Comparing with BMSCCon group, Rats in BMSCKl group had lower SCr and MDA but higher SOD. Both HE and TUNEL score of renal tissue in BMSCKl group were lower than that of BMSCCon group. Western blot indicated that FoxO1 was upregulated, while p-FoxO1 was downregulated in post-hypoxia NRK-52E cells. Conclusions: BMSCs transfected with Klotho gene can further ameliorate RIRI. The possible mechanism may be attributed to the upregulation of SOD in NRK-52E caused by Klotho-FoxO1 axis. |
| first_indexed | 2024-12-22T01:25:01Z |
| format | Article |
| id | doaj.art-b4ef3a35bc4c4df4af5051920b1c9f99 |
| institution | Directory Open Access Journal |
| issn | 0886-022X 1525-6049 |
| language | English |
| last_indexed | 2024-12-22T01:25:01Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Renal Failure |
| spelling | doaj.art-b4ef3a35bc4c4df4af5051920b1c9f992022-12-21T18:43:38ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492019-01-0141117518210.1080/0886022X.2019.15881311588131Protective effect of bone marrow mesenchymal stem cells modified with klotho on renal ischemia-reperfusion injuryLi-Bo Xie0Xi Chen1Bo Chen2Xian-Ding Wang3Rui Jiang4Yi-Ping Lu5The Affiliated Hospital of Southwest Medical UniversityEast China Normal UniversitySouthwest Medical UniversityThe Institution of Urology, West China Hospital, Sichuan UniversityThe Affiliated Hospital of Southwest Medical UniversityThe Institution of Urology, West China Hospital, Sichuan UniversityObjective: To detect the combination protective effect of bone marrow mesenchymal stem cells (BMSCs) and Klotho gene on the renal ischemia-reperfusion injury (RIRI). Methods: BMSCs isolated from rats were transfected with Klotho gene to form BMSCKl. We injected BMSCKl to allogenic rat RIRI model. After 24 h and 72 h, we detected the serum creatinine (SCr), malondialdehyde (MDA), and superoxide dismutase (SOD) in renal tissue, Hematoxylin-eosin (HE) staining, and TUNEL of renal pathology. The expression of FoxO1 and p-FoxO1 in post-hypoxia tubular epithelial cells of normal rat kidney (NRK-52E) were detected by Western blot after cocultured with BMSCKl. Results: Comparing with BMSCCon group, Rats in BMSCKl group had lower SCr and MDA but higher SOD. Both HE and TUNEL score of renal tissue in BMSCKl group were lower than that of BMSCCon group. Western blot indicated that FoxO1 was upregulated, while p-FoxO1 was downregulated in post-hypoxia NRK-52E cells. Conclusions: BMSCs transfected with Klotho gene can further ameliorate RIRI. The possible mechanism may be attributed to the upregulation of SOD in NRK-52E caused by Klotho-FoxO1 axis.http://dx.doi.org/10.1080/0886022X.2019.1588131renal ischemia-reperfusion injurybone marrow mesenchymal stem cellsgene transfectionklothofoxo1 |
| spellingShingle | Li-Bo Xie Xi Chen Bo Chen Xian-Ding Wang Rui Jiang Yi-Ping Lu Protective effect of bone marrow mesenchymal stem cells modified with klotho on renal ischemia-reperfusion injury Renal Failure renal ischemia-reperfusion injury bone marrow mesenchymal stem cells gene transfection klotho foxo1 |
| title | Protective effect of bone marrow mesenchymal stem cells modified with klotho on renal ischemia-reperfusion injury |
| title_full | Protective effect of bone marrow mesenchymal stem cells modified with klotho on renal ischemia-reperfusion injury |
| title_fullStr | Protective effect of bone marrow mesenchymal stem cells modified with klotho on renal ischemia-reperfusion injury |
| title_full_unstemmed | Protective effect of bone marrow mesenchymal stem cells modified with klotho on renal ischemia-reperfusion injury |
| title_short | Protective effect of bone marrow mesenchymal stem cells modified with klotho on renal ischemia-reperfusion injury |
| title_sort | protective effect of bone marrow mesenchymal stem cells modified with klotho on renal ischemia reperfusion injury |
| topic | renal ischemia-reperfusion injury bone marrow mesenchymal stem cells gene transfection klotho foxo1 |
| url | http://dx.doi.org/10.1080/0886022X.2019.1588131 |
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