Oyster Biodeposition Alleviates Sediment Nutrient Overload: A Case Study at Shenzhen Bay, China
Oysters are ecological engineers, and previous studies have examined their role as competent facilitators of ecological restoration. However, the decisive role of oysters in the aquatic environment is still debatable because oyster biodeposition (OBD) may also increase the nutrients enriched in sedi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.716201/full |
| _version_ | 1818842930968788992 |
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| author | Autif Hussain Mangi Autif Hussain Mangi Qi Yan Qi Yan Xiao Song Xiao Song Junting Song Junting Song Xia Lan Jin Zhou Zhong-Hua Cai |
| author_facet | Autif Hussain Mangi Autif Hussain Mangi Qi Yan Qi Yan Xiao Song Xiao Song Junting Song Junting Song Xia Lan Jin Zhou Zhong-Hua Cai |
| author_sort | Autif Hussain Mangi |
| collection | DOAJ |
| description | Oysters are ecological engineers, and previous studies have examined their role as competent facilitators of ecological restoration. However, the decisive role of oysters in the aquatic environment is still debatable because oyster biodeposition (OBD) may also increase the nutrients enriched in sediments. In order to better interpret this problem, we sampled sediment cores from representative oyster culture areas and uncultured areas in Shenzhen Bay. The results have shown that the TOC (total organic carbon) and TN (total nitrogen) decreased significantly (p < 0.05) at the surface sediment layer (0–20-cm deep) and the sediment layer (20–40-cm deep) of the oyster site compared with the reference site. The decreased TOC and TN were also observed at 60- to 100-cm sediment depth in the oyster site. This indicated that the OBD significantly impacted the concentration of TOC and TN in the sediment. To confirm the alleviative role of OBD, we conducted stable isotope (δ13C and δ15N) analyses, which further demonstrated the presence of heavier and less lighter forms of organic carbon and nitrogen sediment. The surface sediment layer (0–20 cm) at the oyster site showed 8% more δ13C‰ compared with the control site (p < 0.05), reflecting the reduction in the TOC. In order to reveal the potential microbial mechanisms involved in OBD, we performed a functional analysis using the Geochip5 advanced microarray technology. Regarding carbon metabolism, we observed that genes (encoding pullulanase, glucoamylase, exoglucanase, cellobiase, and xylanase) involved in the degradation of relatively labile C-based molecules (e.g., starch, cellulose, and hemicellulose) were highly represented in an experimental area (p < 0.05). In addition, microbes in the experimental area exhibited a greater capacity for degrading recalcitrant C (e.g., lignin), which involves glyoxal oxidase (glx), manganese peroxidase (mnp), and phenol oxidase. Among the genes controlling nitrogen metabolism, the genes involved in denitrification, assimilation, ammonification, and nitrification were differentially expressed compared with the control area. These results indicated that microbial metabolic roles might have enhanced the C/N-flux speed and reduced the overall nutrient status. We concluded that OBD alleviates sediment nutrient overload under oyster farming from a microbial ecological perspective in a rapidly urbanized coastal area. |
| first_indexed | 2024-12-19T04:49:48Z |
| format | Article |
| id | doaj.art-b4f3f5abafee415e9b32813546c04651 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-19T04:49:48Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-b4f3f5abafee415e9b32813546c046512022-12-21T20:35:24ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-11-011210.3389/fmicb.2021.716201716201Oyster Biodeposition Alleviates Sediment Nutrient Overload: A Case Study at Shenzhen Bay, ChinaAutif Hussain Mangi0Autif Hussain Mangi1Qi Yan2Qi Yan3Xiao Song4Xiao Song5Junting Song6Junting Song7Xia Lan8Jin Zhou9Zhong-Hua Cai10Shenzhen Public Platform for Screening and Application of Marine Microbial Resources, Institute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, ChinaSchool of Life Sciences, Tsinghua University, Beijing, ChinaShenzhen Public Platform for Screening and Application of Marine Microbial Resources, Institute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, ChinaSchool of Life Sciences, Tsinghua University, Beijing, ChinaShenzhen Public Platform for Screening and Application of Marine Microbial Resources, Institute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, ChinaSchool of Life Sciences, Tsinghua University, Beijing, ChinaShenzhen Public Platform for Screening and Application of Marine Microbial Resources, Institute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, ChinaSchool of Life Sciences, Tsinghua University, Beijing, ChinaShenzhen Public Platform for Screening and Application of Marine Microbial Resources, Institute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, ChinaShenzhen Public Platform for Screening and Application of Marine Microbial Resources, Institute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, ChinaShenzhen Public Platform for Screening and Application of Marine Microbial Resources, Institute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, ChinaOysters are ecological engineers, and previous studies have examined their role as competent facilitators of ecological restoration. However, the decisive role of oysters in the aquatic environment is still debatable because oyster biodeposition (OBD) may also increase the nutrients enriched in sediments. In order to better interpret this problem, we sampled sediment cores from representative oyster culture areas and uncultured areas in Shenzhen Bay. The results have shown that the TOC (total organic carbon) and TN (total nitrogen) decreased significantly (p < 0.05) at the surface sediment layer (0–20-cm deep) and the sediment layer (20–40-cm deep) of the oyster site compared with the reference site. The decreased TOC and TN were also observed at 60- to 100-cm sediment depth in the oyster site. This indicated that the OBD significantly impacted the concentration of TOC and TN in the sediment. To confirm the alleviative role of OBD, we conducted stable isotope (δ13C and δ15N) analyses, which further demonstrated the presence of heavier and less lighter forms of organic carbon and nitrogen sediment. The surface sediment layer (0–20 cm) at the oyster site showed 8% more δ13C‰ compared with the control site (p < 0.05), reflecting the reduction in the TOC. In order to reveal the potential microbial mechanisms involved in OBD, we performed a functional analysis using the Geochip5 advanced microarray technology. Regarding carbon metabolism, we observed that genes (encoding pullulanase, glucoamylase, exoglucanase, cellobiase, and xylanase) involved in the degradation of relatively labile C-based molecules (e.g., starch, cellulose, and hemicellulose) were highly represented in an experimental area (p < 0.05). In addition, microbes in the experimental area exhibited a greater capacity for degrading recalcitrant C (e.g., lignin), which involves glyoxal oxidase (glx), manganese peroxidase (mnp), and phenol oxidase. Among the genes controlling nitrogen metabolism, the genes involved in denitrification, assimilation, ammonification, and nitrification were differentially expressed compared with the control area. These results indicated that microbial metabolic roles might have enhanced the C/N-flux speed and reduced the overall nutrient status. We concluded that OBD alleviates sediment nutrient overload under oyster farming from a microbial ecological perspective in a rapidly urbanized coastal area.https://www.frontiersin.org/articles/10.3389/fmicb.2021.716201/fulloyster biodepositionsediment nutrientalleviative effecteutrophicationShenzhen Bay |
| spellingShingle | Autif Hussain Mangi Autif Hussain Mangi Qi Yan Qi Yan Xiao Song Xiao Song Junting Song Junting Song Xia Lan Jin Zhou Zhong-Hua Cai Oyster Biodeposition Alleviates Sediment Nutrient Overload: A Case Study at Shenzhen Bay, China Frontiers in Microbiology oyster biodeposition sediment nutrient alleviative effect eutrophication Shenzhen Bay |
| title | Oyster Biodeposition Alleviates Sediment Nutrient Overload: A Case Study at Shenzhen Bay, China |
| title_full | Oyster Biodeposition Alleviates Sediment Nutrient Overload: A Case Study at Shenzhen Bay, China |
| title_fullStr | Oyster Biodeposition Alleviates Sediment Nutrient Overload: A Case Study at Shenzhen Bay, China |
| title_full_unstemmed | Oyster Biodeposition Alleviates Sediment Nutrient Overload: A Case Study at Shenzhen Bay, China |
| title_short | Oyster Biodeposition Alleviates Sediment Nutrient Overload: A Case Study at Shenzhen Bay, China |
| title_sort | oyster biodeposition alleviates sediment nutrient overload a case study at shenzhen bay china |
| topic | oyster biodeposition sediment nutrient alleviative effect eutrophication Shenzhen Bay |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.716201/full |
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