Wenjing Zhitong recipe exhibits potential anti-primary dysmenorrhea properties by inhibiting COX2 and PKC signaling pathway in rats induced by estradiol benzoate and oxytocin
Objective: To explore the effect and mechanism of action of the Wenjing Zhitong recipe (WZR) in primary dysmenorrhea (PD) treatment. Methods: Uterine contractions were induced by estradiol benzoate and oxytocin in a PD model and WZR was administrated. The rate of change in uterine contractility and...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Journal of Traditional Chinese Medical Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S209575482300039X |
| _version_ | 1797771587245572096 |
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| author | Jialin Li Xiaoyao Liu Mei Jiang Yantong Xu Chunguo Wang Yujie Hu Song Yang Jian Gao Weiling Wang Ting Wang |
| author_facet | Jialin Li Xiaoyao Liu Mei Jiang Yantong Xu Chunguo Wang Yujie Hu Song Yang Jian Gao Weiling Wang Ting Wang |
| author_sort | Jialin Li |
| collection | DOAJ |
| description | Objective: To explore the effect and mechanism of action of the Wenjing Zhitong recipe (WZR) in primary dysmenorrhea (PD) treatment. Methods: Uterine contractions were induced by estradiol benzoate and oxytocin in a PD model and WZR was administrated. The rate of change in uterine contractility and the writhing test were used to evaluate the effects of WZR. The serum levels of prostaglandin F2α (PGF2α) and prostaglandin E2 (PGE2), and the activity of cyclooxygenase-2 (COX2) were detected by enzyme-linked immunosorbent assay (ELISA). The changes in phosphor-phospholipase C (pPLC/PLC), phosphor-protein kinase C (pPKC/PKC), and connexin 43 (CX43) expression were detected using immunohistochemistry and western blot. Results: WZR significantly reduced the rate of change in uterine contractility and writhing times in the PD model. WZR treatment inhibited the enzymatic activity of COX2 and reduced the levels of PGF2α, PGF2α/PGE2 and COX2 in the PD model. WZR also significantly reduced the expression of pPLC/PLC, pPKC/PKC and CX43. Targeting the inhibition of COX2 activity, caffeic acid and 1-acetyl-β-carboline were validated as the active ingredients in WZR responsible for reducing uterine contractions. Conclusion: WZR attenuated PD by inhibiting COX2 activity, downregulating PGF2α/PGE2 expression, and inhibiting the PKC signaling pathway. |
| first_indexed | 2024-03-12T21:39:46Z |
| format | Article |
| id | doaj.art-b4f4df9fd4734211aa577099b5e3063f |
| institution | Directory Open Access Journal |
| issn | 2095-7548 |
| language | English |
| last_indexed | 2024-03-12T21:39:46Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional Chinese Medical Sciences |
| spelling | doaj.art-b4f4df9fd4734211aa577099b5e3063f2023-07-27T05:55:44ZengElsevierJournal of Traditional Chinese Medical Sciences2095-75482023-07-01103296309Wenjing Zhitong recipe exhibits potential anti-primary dysmenorrhea properties by inhibiting COX2 and PKC signaling pathway in rats induced by estradiol benzoate and oxytocinJialin Li0Xiaoyao Liu1Mei Jiang2Yantong Xu3Chunguo Wang4Yujie Hu5Song Yang6Jian Gao7Weiling Wang8Ting Wang9School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, ChinaBeijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China; Key Laboratory of Famous Doctors and Famous Prescriptions, State Administration of Traditional Chinese Medicine, Beijing 100020, ChinaBeijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China; Key Laboratory of Famous Doctors and Famous Prescriptions, State Administration of Traditional Chinese Medicine, Beijing 100020, ChinaBeijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China; Key Laboratory of Famous Doctors and Famous Prescriptions, State Administration of Traditional Chinese Medicine, Beijing 100020, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, ChinaBeijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China; Key Laboratory of Famous Doctors and Famous Prescriptions, State Administration of Traditional Chinese Medicine, Beijing 100020, ChinaBeijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China; Key Laboratory of Famous Doctors and Famous Prescriptions, State Administration of Traditional Chinese Medicine, Beijing 100020, ChinaBeijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China; Key Laboratory of Famous Doctors and Famous Prescriptions, State Administration of Traditional Chinese Medicine, Beijing 100020, ChinaBeijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China; Key Laboratory of Famous Doctors and Famous Prescriptions, State Administration of Traditional Chinese Medicine, Beijing 100020, China; Corresponding author.Objective: To explore the effect and mechanism of action of the Wenjing Zhitong recipe (WZR) in primary dysmenorrhea (PD) treatment. Methods: Uterine contractions were induced by estradiol benzoate and oxytocin in a PD model and WZR was administrated. The rate of change in uterine contractility and the writhing test were used to evaluate the effects of WZR. The serum levels of prostaglandin F2α (PGF2α) and prostaglandin E2 (PGE2), and the activity of cyclooxygenase-2 (COX2) were detected by enzyme-linked immunosorbent assay (ELISA). The changes in phosphor-phospholipase C (pPLC/PLC), phosphor-protein kinase C (pPKC/PKC), and connexin 43 (CX43) expression were detected using immunohistochemistry and western blot. Results: WZR significantly reduced the rate of change in uterine contractility and writhing times in the PD model. WZR treatment inhibited the enzymatic activity of COX2 and reduced the levels of PGF2α, PGF2α/PGE2 and COX2 in the PD model. WZR also significantly reduced the expression of pPLC/PLC, pPKC/PKC and CX43. Targeting the inhibition of COX2 activity, caffeic acid and 1-acetyl-β-carboline were validated as the active ingredients in WZR responsible for reducing uterine contractions. Conclusion: WZR attenuated PD by inhibiting COX2 activity, downregulating PGF2α/PGE2 expression, and inhibiting the PKC signaling pathway.http://www.sciencedirect.com/science/article/pii/S209575482300039XWenjing zhitong recipePrimary dysmenorrheaNetwork pharmacologyPGF2αPKC system |
| spellingShingle | Jialin Li Xiaoyao Liu Mei Jiang Yantong Xu Chunguo Wang Yujie Hu Song Yang Jian Gao Weiling Wang Ting Wang Wenjing Zhitong recipe exhibits potential anti-primary dysmenorrhea properties by inhibiting COX2 and PKC signaling pathway in rats induced by estradiol benzoate and oxytocin Journal of Traditional Chinese Medical Sciences Wenjing zhitong recipe Primary dysmenorrhea Network pharmacology PGF2α PKC system |
| title | Wenjing Zhitong recipe exhibits potential anti-primary dysmenorrhea properties by inhibiting COX2 and PKC signaling pathway in rats induced by estradiol benzoate and oxytocin |
| title_full | Wenjing Zhitong recipe exhibits potential anti-primary dysmenorrhea properties by inhibiting COX2 and PKC signaling pathway in rats induced by estradiol benzoate and oxytocin |
| title_fullStr | Wenjing Zhitong recipe exhibits potential anti-primary dysmenorrhea properties by inhibiting COX2 and PKC signaling pathway in rats induced by estradiol benzoate and oxytocin |
| title_full_unstemmed | Wenjing Zhitong recipe exhibits potential anti-primary dysmenorrhea properties by inhibiting COX2 and PKC signaling pathway in rats induced by estradiol benzoate and oxytocin |
| title_short | Wenjing Zhitong recipe exhibits potential anti-primary dysmenorrhea properties by inhibiting COX2 and PKC signaling pathway in rats induced by estradiol benzoate and oxytocin |
| title_sort | wenjing zhitong recipe exhibits potential anti primary dysmenorrhea properties by inhibiting cox2 and pkc signaling pathway in rats induced by estradiol benzoate and oxytocin |
| topic | Wenjing zhitong recipe Primary dysmenorrhea Network pharmacology PGF2α PKC system |
| url | http://www.sciencedirect.com/science/article/pii/S209575482300039X |
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