Circ_0027885 sponges miR-203-3p to regulate RUNX2 expression and alleviates osteoporosis progression
Abstract Background Osteoporosis (OP) is a progressive metabolic disorder that is difficult to cure clinically. The molecular mechanisms of OP urgently need to be further examined. This study was designed to explore the potential function of circ_0027885 during osteogenic differentiation, as well as...
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | BMC Musculoskeletal Disorders |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12891-023-07122-1 |
| _version_ | 1797363755632295936 |
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| author | Shuhua Fang Dingwen Cao Zhanpo Wu Jie Chen Yafei Huang Ying Shen Zengxin Gao |
| author_facet | Shuhua Fang Dingwen Cao Zhanpo Wu Jie Chen Yafei Huang Ying Shen Zengxin Gao |
| author_sort | Shuhua Fang |
| collection | DOAJ |
| description | Abstract Background Osteoporosis (OP) is a progressive metabolic disorder that is difficult to cure clinically. The molecular mechanisms of OP urgently need to be further examined. This study was designed to explore the potential function of circ_0027885 during osteogenic differentiation, as well as the systematic interactions among circ_0027885, miR-203-3p and runt-related transcription factor 2 (RUNX2). Methods Relative levels of circ_0027885, miR-203-3p and RUNX2 were analyzed with RT-qPCR and western blotting. Alizarin red staining was performed to detect the mineralization ability under the control of circ_0027885 and miR-203-3p. Dual-luciferase reporter gene assay was conducted to examine the combination among circ_0027885, miR-203-3p and RUNX2. Results Our research demonstrated that circ_0027885 was significantly increased during hBMSCs differentiation. Overexpression of circ_0027885 notably facilitated osteogenic differentiation and upregulated RUNX2 expression, while knockdown of circ_0027885 reversed the above results. Through prediction on bioinformatics analysis, miR-203-3p was the target binding circ_0027885, and RUNX2 was the potential target of miR-203-3p. Subsequently, these changes induced by the overexpression of circ_0027885 were reversed upon addition of miR-203-3p mimic. Conclusions Circ_0027885 could sponge miR-203-3p to regulate RUNX2 expression and alleviate osteoporosis progression. |
| first_indexed | 2024-03-08T16:25:38Z |
| format | Article |
| id | doaj.art-b4f53c55b6564758a511592ae5a330d9 |
| institution | Directory Open Access Journal |
| issn | 1471-2474 |
| language | English |
| last_indexed | 2024-03-08T16:25:38Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Musculoskeletal Disorders |
| spelling | doaj.art-b4f53c55b6564758a511592ae5a330d92024-01-07T12:04:08ZengBMCBMC Musculoskeletal Disorders1471-24742024-01-0125111110.1186/s12891-023-07122-1Circ_0027885 sponges miR-203-3p to regulate RUNX2 expression and alleviates osteoporosis progressionShuhua Fang0Dingwen Cao1Zhanpo Wu2Jie Chen3Yafei Huang4Ying Shen5Zengxin Gao6Department of Pharmacy, Nanjing Lishui People’s Hospital, Zhongda Hospital Lishui Branch Southeast UniversityDepartment of Pharmacy, Nanjing Lishui People’s Hospital, Zhongda Hospital Lishui Branch Southeast UniversityDepartment of Orthopedics, Nanjing Lishui People’s Hospital, Zhongda Hospital Lishui Branch Southeast UniversityDepartment of Pharmacy, Nanjing Lishui People’s Hospital, Zhongda Hospital Lishui Branch Southeast UniversityDepartment of Pharmacy, Nanjing Lishui People’s Hospital, Zhongda Hospital Lishui Branch Southeast UniversityDepartment of Pharmacy, Nanjing Lishui People’s Hospital, Zhongda Hospital Lishui Branch Southeast UniversityDepartment of Orthopedics, Nanjing Lishui People’s Hospital, Zhongda Hospital Lishui Branch Southeast UniversityAbstract Background Osteoporosis (OP) is a progressive metabolic disorder that is difficult to cure clinically. The molecular mechanisms of OP urgently need to be further examined. This study was designed to explore the potential function of circ_0027885 during osteogenic differentiation, as well as the systematic interactions among circ_0027885, miR-203-3p and runt-related transcription factor 2 (RUNX2). Methods Relative levels of circ_0027885, miR-203-3p and RUNX2 were analyzed with RT-qPCR and western blotting. Alizarin red staining was performed to detect the mineralization ability under the control of circ_0027885 and miR-203-3p. Dual-luciferase reporter gene assay was conducted to examine the combination among circ_0027885, miR-203-3p and RUNX2. Results Our research demonstrated that circ_0027885 was significantly increased during hBMSCs differentiation. Overexpression of circ_0027885 notably facilitated osteogenic differentiation and upregulated RUNX2 expression, while knockdown of circ_0027885 reversed the above results. Through prediction on bioinformatics analysis, miR-203-3p was the target binding circ_0027885, and RUNX2 was the potential target of miR-203-3p. Subsequently, these changes induced by the overexpression of circ_0027885 were reversed upon addition of miR-203-3p mimic. Conclusions Circ_0027885 could sponge miR-203-3p to regulate RUNX2 expression and alleviate osteoporosis progression.https://doi.org/10.1186/s12891-023-07122-1Circ_0027885miR-203-3pRUNX2OsteoporosisOsteogenic differentiation |
| spellingShingle | Shuhua Fang Dingwen Cao Zhanpo Wu Jie Chen Yafei Huang Ying Shen Zengxin Gao Circ_0027885 sponges miR-203-3p to regulate RUNX2 expression and alleviates osteoporosis progression BMC Musculoskeletal Disorders Circ_0027885 miR-203-3p RUNX2 Osteoporosis Osteogenic differentiation |
| title | Circ_0027885 sponges miR-203-3p to regulate RUNX2 expression and alleviates osteoporosis progression |
| title_full | Circ_0027885 sponges miR-203-3p to regulate RUNX2 expression and alleviates osteoporosis progression |
| title_fullStr | Circ_0027885 sponges miR-203-3p to regulate RUNX2 expression and alleviates osteoporosis progression |
| title_full_unstemmed | Circ_0027885 sponges miR-203-3p to regulate RUNX2 expression and alleviates osteoporosis progression |
| title_short | Circ_0027885 sponges miR-203-3p to regulate RUNX2 expression and alleviates osteoporosis progression |
| title_sort | circ 0027885 sponges mir 203 3p to regulate runx2 expression and alleviates osteoporosis progression |
| topic | Circ_0027885 miR-203-3p RUNX2 Osteoporosis Osteogenic differentiation |
| url | https://doi.org/10.1186/s12891-023-07122-1 |
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