Comparative Evaluation of the Antimicrobial and Mucus Induction Properties of Selected <i>Bacillus</i> Strains against Enterotoxigenic <i>Escherichia coli</i>
Probiotics have been shown to bind to host receptors, which are important for pathogen adhesion and induce the host’s production of defence factors. They can activate the goblet-cell-derived production of mucins, a major component of the mucus layer and a physical barrier participating in limiting t...
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| Format: | Article |
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MDPI AG
2020-11-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/9/12/849 |
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| author | Natalia Bravo Santano Erik Juncker Boll Lena Catrine Capern Tomasz Maciej Cieplak Enver Keleszade Michal Letek Adele Costabile |
| author_facet | Natalia Bravo Santano Erik Juncker Boll Lena Catrine Capern Tomasz Maciej Cieplak Enver Keleszade Michal Letek Adele Costabile |
| author_sort | Natalia Bravo Santano |
| collection | DOAJ |
| description | Probiotics have been shown to bind to host receptors, which are important for pathogen adhesion and induce the host’s production of defence factors. They can activate the goblet-cell-derived production of mucins, a major component of the mucus layer and a physical barrier participating in limiting the proximity of microorganisms to the epithelial layer. In the last decade, <i>Bacillus</i> spp. strains have gained interest in human and animal health due to their tolerance and stability under gastrointestinal tract conditions. Moreover, <i>Bacillus</i> spp. strains can also produce various antimicrobial peptides that can support their use as commercial probiotic supplements and functional foods. The present study aimed to evaluate and determine the ability of selected <i>Bacillus</i> spp. strains to inhibit the growth of enterotoxigenic <i>Escherichia coli</i> (ETEC) F4 and to reduce binding of ETEC F4 to HT29-16E (mucus-secreting and goblet-like) human intestinal cells. Moreover, mucus production in the HT29 cells in the presence of the <i>Bacillus</i> spp. strains was quantified by ELISA. <i>Bacillus</i> spp. strains (CHCC 15076, CHCC 15516, CHCC 15541, and CHCC 16872) significantly inhibited the growth of ETEC F4. Moreover, the ability of the probiotic <i>Bacillus</i> spp. strains to stimulate mucin release was highly strain dependent. The treatment with <i>Bacillus subtilis</i> CHCC 15541 resulted in a significant increase of both MUC2 and MUC3 in HT29-16E cells. Therefore, this strain could be an up-and-coming candidate for developing commercial probiotic supplements to prevent infections caused by ETEC F4 and, potentially, other pathogens. |
| first_indexed | 2024-03-10T14:31:22Z |
| format | Article |
| id | doaj.art-b4f5737d3d52482cae46d218ea6950ea |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T14:31:22Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-b4f5737d3d52482cae46d218ea6950ea2023-11-20T22:37:10ZengMDPI AGAntibiotics2079-63822020-11-0191284910.3390/antibiotics9120849Comparative Evaluation of the Antimicrobial and Mucus Induction Properties of Selected <i>Bacillus</i> Strains against Enterotoxigenic <i>Escherichia coli</i>Natalia Bravo Santano0Erik Juncker Boll1Lena Catrine Capern2Tomasz Maciej Cieplak3Enver Keleszade4Michal Letek5Adele Costabile6Department of Life Sciences, University of Roehampton, London SW154JD, UKAnimal Health Innovation, Chr. Hansen A/S, 2970 Hørsholm, DenmarkAnimal Health Innovation, Chr. Hansen A/S, 2970 Hørsholm, DenmarkAnimal Health Innovation, Chr. Hansen A/S, 2970 Hørsholm, DenmarkDepartment of Life Sciences, University of Roehampton, London SW154JD, UKDepartamento de Biología Molecular, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, 24071 León, SpainDepartment of Life Sciences, University of Roehampton, London SW154JD, UKProbiotics have been shown to bind to host receptors, which are important for pathogen adhesion and induce the host’s production of defence factors. They can activate the goblet-cell-derived production of mucins, a major component of the mucus layer and a physical barrier participating in limiting the proximity of microorganisms to the epithelial layer. In the last decade, <i>Bacillus</i> spp. strains have gained interest in human and animal health due to their tolerance and stability under gastrointestinal tract conditions. Moreover, <i>Bacillus</i> spp. strains can also produce various antimicrobial peptides that can support their use as commercial probiotic supplements and functional foods. The present study aimed to evaluate and determine the ability of selected <i>Bacillus</i> spp. strains to inhibit the growth of enterotoxigenic <i>Escherichia coli</i> (ETEC) F4 and to reduce binding of ETEC F4 to HT29-16E (mucus-secreting and goblet-like) human intestinal cells. Moreover, mucus production in the HT29 cells in the presence of the <i>Bacillus</i> spp. strains was quantified by ELISA. <i>Bacillus</i> spp. strains (CHCC 15076, CHCC 15516, CHCC 15541, and CHCC 16872) significantly inhibited the growth of ETEC F4. Moreover, the ability of the probiotic <i>Bacillus</i> spp. strains to stimulate mucin release was highly strain dependent. The treatment with <i>Bacillus subtilis</i> CHCC 15541 resulted in a significant increase of both MUC2 and MUC3 in HT29-16E cells. Therefore, this strain could be an up-and-coming candidate for developing commercial probiotic supplements to prevent infections caused by ETEC F4 and, potentially, other pathogens.https://www.mdpi.com/2079-6382/9/12/849<i>Escherichia coli</i>adhesiongoblet-cell-derived mucinsprobiotics<i>Bacillus</i> |
| spellingShingle | Natalia Bravo Santano Erik Juncker Boll Lena Catrine Capern Tomasz Maciej Cieplak Enver Keleszade Michal Letek Adele Costabile Comparative Evaluation of the Antimicrobial and Mucus Induction Properties of Selected <i>Bacillus</i> Strains against Enterotoxigenic <i>Escherichia coli</i> Antibiotics <i>Escherichia coli</i> adhesion goblet-cell-derived mucins probiotics <i>Bacillus</i> |
| title | Comparative Evaluation of the Antimicrobial and Mucus Induction Properties of Selected <i>Bacillus</i> Strains against Enterotoxigenic <i>Escherichia coli</i> |
| title_full | Comparative Evaluation of the Antimicrobial and Mucus Induction Properties of Selected <i>Bacillus</i> Strains against Enterotoxigenic <i>Escherichia coli</i> |
| title_fullStr | Comparative Evaluation of the Antimicrobial and Mucus Induction Properties of Selected <i>Bacillus</i> Strains against Enterotoxigenic <i>Escherichia coli</i> |
| title_full_unstemmed | Comparative Evaluation of the Antimicrobial and Mucus Induction Properties of Selected <i>Bacillus</i> Strains against Enterotoxigenic <i>Escherichia coli</i> |
| title_short | Comparative Evaluation of the Antimicrobial and Mucus Induction Properties of Selected <i>Bacillus</i> Strains against Enterotoxigenic <i>Escherichia coli</i> |
| title_sort | comparative evaluation of the antimicrobial and mucus induction properties of selected i bacillus i strains against enterotoxigenic i escherichia coli i |
| topic | <i>Escherichia coli</i> adhesion goblet-cell-derived mucins probiotics <i>Bacillus</i> |
| url | https://www.mdpi.com/2079-6382/9/12/849 |
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