Low-Temperature Conservation of Collection Cholera Vibrio Strains
Objective of the study is to evaluate cholera vibrio resistance to freezing and freeze preservation at -70 °C in the presence of cryoprotectors. The study outlines the results of storage of seven different V. cholerae strains within three-years term in the protective media containing glycerin or lac...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2014-12-01
|
| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/183 |
| _version_ | 1797220892560850944 |
|---|---|
| author | I. V. Gracheva A.V. Low Osin |
| author_facet | I. V. Gracheva A.V. Low Osin |
| author_sort | I. V. Gracheva |
| collection | DOAJ |
| description | Objective of the study is to evaluate cholera vibrio resistance to freezing and freeze preservation at -70 °C in the presence of cryoprotectors. The study outlines the results of storage of seven different V. cholerae strains within three-years term in the protective media containing glycerin or lactose (10, 20, 30 % aquatic solutions of glycerin, meat-peptone broth with 10 % of glycerin, a medium with 15 % of lactose and 3 % of gelatin) as cryoprotectors. Demonstrated is the fact that cholera vibrio death occurs during freezing-refreezing procedure and preservation of samples. After three-year conservation the numbers of living cells decrease in all types of media, but to different extent. The highest survival rate in cholera vibrios after freezing-refreezing procedure and freeze preservation in accordance with applicable protocols is observed in lactose-gelatin medium. When using aquatic solution of glycerin as protective medium, optimum turns out to be the cryoprotector concentration equal to 20 %. After three-year preservation at -70 °C all the strains, irrespective of the protective medium composition, retain their basic diagnostically significant phenotypic characteristics. Thus, cholera vibrios can be conserved free from subcultures within three-year term (period of observation) without alterations to their basic diagnostic properties in all tested protective media but at -70 °C. |
| first_indexed | 2024-03-08T13:43:43Z |
| format | Article |
| id | doaj.art-b4f98a7a0217476cb0dbaebcca1e730e |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2024-04-24T12:56:46Z |
| publishDate | 2014-12-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-b4f98a7a0217476cb0dbaebcca1e730e2024-04-05T16:47:10ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2014-12-0104394210.21055/0370-1069-2014-4-39-42183Low-Temperature Conservation of Collection Cholera Vibrio StrainsI. V. Gracheva0A.V. Low Osin1Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Objective of the study is to evaluate cholera vibrio resistance to freezing and freeze preservation at -70 °C in the presence of cryoprotectors. The study outlines the results of storage of seven different V. cholerae strains within three-years term in the protective media containing glycerin or lactose (10, 20, 30 % aquatic solutions of glycerin, meat-peptone broth with 10 % of glycerin, a medium with 15 % of lactose and 3 % of gelatin) as cryoprotectors. Demonstrated is the fact that cholera vibrio death occurs during freezing-refreezing procedure and preservation of samples. After three-year conservation the numbers of living cells decrease in all types of media, but to different extent. The highest survival rate in cholera vibrios after freezing-refreezing procedure and freeze preservation in accordance with applicable protocols is observed in lactose-gelatin medium. When using aquatic solution of glycerin as protective medium, optimum turns out to be the cryoprotector concentration equal to 20 %. After three-year preservation at -70 °C all the strains, irrespective of the protective medium composition, retain their basic diagnostically significant phenotypic characteristics. Thus, cholera vibrios can be conserved free from subcultures within three-year term (period of observation) without alterations to their basic diagnostic properties in all tested protective media but at -70 °C.https://journal.microbe.ru/jour/article/view/183холерные вибрионызамораживаниенизкотемпературная консервациякриопротекторыжизнеспособностьcholera vibriosfreezinglow-temperature conservationcryoprotectorssurvivability |
| spellingShingle | I. V. Gracheva A.V. Low Osin Low-Temperature Conservation of Collection Cholera Vibrio Strains Проблемы особо опасных инфекций холерные вибрионы замораживание низкотемпературная консервация криопротекторы жизнеспособность cholera vibrios freezing low-temperature conservation cryoprotectors survivability |
| title | Low-Temperature Conservation of Collection Cholera Vibrio Strains |
| title_full | Low-Temperature Conservation of Collection Cholera Vibrio Strains |
| title_fullStr | Low-Temperature Conservation of Collection Cholera Vibrio Strains |
| title_full_unstemmed | Low-Temperature Conservation of Collection Cholera Vibrio Strains |
| title_short | Low-Temperature Conservation of Collection Cholera Vibrio Strains |
| title_sort | low temperature conservation of collection cholera vibrio strains |
| topic | холерные вибрионы замораживание низкотемпературная консервация криопротекторы жизнеспособность cholera vibrios freezing low-temperature conservation cryoprotectors survivability |
| url | https://journal.microbe.ru/jour/article/view/183 |
| work_keys_str_mv | AT ivgracheva lowtemperatureconservationofcollectioncholeravibriostrains AT avlowosin lowtemperatureconservationofcollectioncholeravibriostrains |