Chromosome positioning in interphase nuclei of hematopoietic stem cell and myeloid precursor
Human myelopoiesis is an intriguing biological process during which multipotent stem cells limit their differentiation potential generating precursors that evolve into terminally differentiated cells. The differentiation process is correlated with differential gene expression and changes in nuclear...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-04-01
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| Series: | Hematology Reports |
| Subjects: | |
| Online Access: | http://www.pagepress.org/journals/index.php/hr/article/view/7515 |
| _version_ | 1797936772487839744 |
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| author | Mariana Lomiento Fabiana Mammoli Emilia Maria Cristina Mazza Silvio Bicciato Sergio Ferrari |
| author_facet | Mariana Lomiento Fabiana Mammoli Emilia Maria Cristina Mazza Silvio Bicciato Sergio Ferrari |
| author_sort | Mariana Lomiento |
| collection | DOAJ |
| description | Human myelopoiesis is an intriguing biological process during which multipotent stem cells limit their differentiation potential generating precursors that evolve into terminally differentiated cells. The differentiation process is correlated with differential gene expression and changes in nuclear architecture. In interphase, chromosomes are distinct entities known as chromosome territories and they show a radial localization that could result in a constrain of inter-homologous distance. This element plays a role in genome stability and gene expression. Here, we provide the first experimental evidence of 3D chromosomal arrangement considering two steps of human normal myelopoiesis. Specifically, multicolor 3D-FISH and 3D image analysis revealed that, in both normal human hematopoietic stem cells and myelod precursors CD14-, chromosomal position is correlated with gene density. However, we observed that inter-homologue distances are totally different during differentiation. This could be associated with differential gene expression that we found comparing the two cell types. Our results disclose an unprecedented framework relevant for deciphering the genomic mechanisms at the base of normal human myelopoiesis. |
| first_indexed | 2024-04-10T18:34:30Z |
| format | Article |
| id | doaj.art-b4fca2666df94b7286c191e71291ea28 |
| institution | Directory Open Access Journal |
| issn | 2038-8322 2038-8330 |
| language | English |
| last_indexed | 2024-04-10T18:34:30Z |
| publishDate | 2018-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Hematology Reports |
| spelling | doaj.art-b4fca2666df94b7286c191e71291ea282023-02-02T02:01:49ZengMDPI AGHematology Reports2038-83222038-83302018-04-0110110.4081/hr.2018.75153906Chromosome positioning in interphase nuclei of hematopoietic stem cell and myeloid precursorMariana Lomiento0Fabiana Mammoli1Emilia Maria Cristina Mazza2Silvio Bicciato3Sergio Ferrari4Department of Life Sciences, University of Modena and Reggio Emilia, ModenaDepartment of Life Sciences, University of Modena and Reggio Emilia, ModenaDepartment of Life Sciences, University of Modena and Reggio Emilia, ModenaDepartment of Life Sciences, University of Modena and Reggio Emilia, ModenaDepartment of Life Sciences, University of Modena and Reggio Emilia, ModenaHuman myelopoiesis is an intriguing biological process during which multipotent stem cells limit their differentiation potential generating precursors that evolve into terminally differentiated cells. The differentiation process is correlated with differential gene expression and changes in nuclear architecture. In interphase, chromosomes are distinct entities known as chromosome territories and they show a radial localization that could result in a constrain of inter-homologous distance. This element plays a role in genome stability and gene expression. Here, we provide the first experimental evidence of 3D chromosomal arrangement considering two steps of human normal myelopoiesis. Specifically, multicolor 3D-FISH and 3D image analysis revealed that, in both normal human hematopoietic stem cells and myelod precursors CD14-, chromosomal position is correlated with gene density. However, we observed that inter-homologue distances are totally different during differentiation. This could be associated with differential gene expression that we found comparing the two cell types. Our results disclose an unprecedented framework relevant for deciphering the genomic mechanisms at the base of normal human myelopoiesis.http://www.pagepress.org/journals/index.php/hr/article/view/7515Hematopoietic Stem CellMyelopoiesisgene expression3D FISHchromosome territory |
| spellingShingle | Mariana Lomiento Fabiana Mammoli Emilia Maria Cristina Mazza Silvio Bicciato Sergio Ferrari Chromosome positioning in interphase nuclei of hematopoietic stem cell and myeloid precursor Hematology Reports Hematopoietic Stem Cell Myelopoiesis gene expression 3D FISH chromosome territory |
| title | Chromosome positioning in interphase nuclei of hematopoietic stem cell and myeloid precursor |
| title_full | Chromosome positioning in interphase nuclei of hematopoietic stem cell and myeloid precursor |
| title_fullStr | Chromosome positioning in interphase nuclei of hematopoietic stem cell and myeloid precursor |
| title_full_unstemmed | Chromosome positioning in interphase nuclei of hematopoietic stem cell and myeloid precursor |
| title_short | Chromosome positioning in interphase nuclei of hematopoietic stem cell and myeloid precursor |
| title_sort | chromosome positioning in interphase nuclei of hematopoietic stem cell and myeloid precursor |
| topic | Hematopoietic Stem Cell Myelopoiesis gene expression 3D FISH chromosome territory |
| url | http://www.pagepress.org/journals/index.php/hr/article/view/7515 |
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