Two Synthetic Peptides Corresponding to the Human Follicle-Stimulating Hormone β-Subunit Promoted Reproductive Functions in Mice
A follicle stimulating hormone (FSH) is widely used in the assisted reproduction and a synthetic peptide corresponding to a receptor binding region of the human (h) FSH-β-(34–37) (TRDL) modulated reproduction. Furthermore, a 13-amino acid sequence corresponding to hFSH-β-(37–49) (LVYKDPARPKIQK) was...
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/19/11735 |
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| author | Xingfa Han Xinyu Bai Huan Yao Weihao Chen Fengyan Meng Xiaohan Cao Yong Zhuo Lun Hua Guixian Bu Xiaogang Du Qiuxia Liang Xianyin Zeng |
| author_facet | Xingfa Han Xinyu Bai Huan Yao Weihao Chen Fengyan Meng Xiaohan Cao Yong Zhuo Lun Hua Guixian Bu Xiaogang Du Qiuxia Liang Xianyin Zeng |
| author_sort | Xingfa Han |
| collection | DOAJ |
| description | A follicle stimulating hormone (FSH) is widely used in the assisted reproduction and a synthetic peptide corresponding to a receptor binding region of the human (h) FSH-β-(34–37) (TRDL) modulated reproduction. Furthermore, a 13-amino acid sequence corresponding to hFSH-β-(37–49) (LVYKDPARPKIQK) was recently identified as the receptor binding site. We hypothesized that the synthetic peptides corresponding to hFSH-β-(37–49) and hFSH-β-(34–49), created by merging hFSH-β-(34–37) and hFSH-β-(37–49), modulate the reproductive functions, with the longer peptide being more biologically active. In male or female prepubertal mice, a single injection of 200 μg/g BW ip of hFSH-β-(37–49) or hFSH-β-(34–49) hastened (<i>p</i> < 0.05) puberty, whereas the same treatments given daily for 4 d promoted (<i>p</i> < 0.05) the gonadal steroidogenesis and gamete formation. In addition of either peptide to the in vitro cell cultures, promoted (<i>p</i> < 0.05) the proliferation of primary murine granulosa cells and the estradiol production by upregulating the expression of <i>Ccnd2</i> and <i>Cyp19a1</i>, respectively. In adult female mice, 200 μg/g BW ip of either peptide during diestrus antagonized the FSH-stimulated estradiol increase and uterine weight gain during proestrus. Furthermore, hFSH-β-(34–49) was a more potent (<i>p</i> < 0.05) reproductive modulator than hFSH-β-(37–49), both in vivo and in vitro. We concluded that hFSH-β-(37–49) and especially hFSH-β-(34–49), have the potential for reproductive modulation. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:38:29Z |
| publishDate | 2022-10-01 |
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| spelling | doaj.art-b4fcb795ecb0402facb8803dda1361652023-11-23T20:38:33ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123191173510.3390/ijms231911735Two Synthetic Peptides Corresponding to the Human Follicle-Stimulating Hormone β-Subunit Promoted Reproductive Functions in MiceXingfa Han0Xinyu Bai1Huan Yao2Weihao Chen3Fengyan Meng4Xiaohan Cao5Yong Zhuo6Lun Hua7Guixian Bu8Xiaogang Du9Qiuxia Liang10Xianyin Zeng11Isotope Research Laboratory, Biological Engineering and Application Biology Department, Sichuan Agricultural University, Ya’an 625014, ChinaIsotope Research Laboratory, Biological Engineering and Application Biology Department, Sichuan Agricultural University, Ya’an 625014, ChinaIsotope Research Laboratory, Biological Engineering and Application Biology Department, Sichuan Agricultural University, Ya’an 625014, ChinaIsotope Research Laboratory, Biological Engineering and Application Biology Department, Sichuan Agricultural University, Ya’an 625014, ChinaIsotope Research Laboratory, Biological Engineering and Application Biology Department, Sichuan Agricultural University, Ya’an 625014, ChinaIsotope Research Laboratory, Biological Engineering and Application Biology Department, Sichuan Agricultural University, Ya’an 625014, ChinaKey Laboratory for Animal Disease-Resistant Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University, Ya’an 625014, ChinaKey Laboratory for Animal Disease-Resistant Nutrition of the Ministry of Education of China, Animal Nutrition Institute, Sichuan Agricultural University, Ya’an 625014, ChinaIsotope Research Laboratory, Biological Engineering and Application Biology Department, Sichuan Agricultural University, Ya’an 625014, ChinaIsotope Research Laboratory, Biological Engineering and Application Biology Department, Sichuan Agricultural University, Ya’an 625014, ChinaIsotope Research Laboratory, Biological Engineering and Application Biology Department, Sichuan Agricultural University, Ya’an 625014, ChinaIsotope Research Laboratory, Biological Engineering and Application Biology Department, Sichuan Agricultural University, Ya’an 625014, ChinaA follicle stimulating hormone (FSH) is widely used in the assisted reproduction and a synthetic peptide corresponding to a receptor binding region of the human (h) FSH-β-(34–37) (TRDL) modulated reproduction. Furthermore, a 13-amino acid sequence corresponding to hFSH-β-(37–49) (LVYKDPARPKIQK) was recently identified as the receptor binding site. We hypothesized that the synthetic peptides corresponding to hFSH-β-(37–49) and hFSH-β-(34–49), created by merging hFSH-β-(34–37) and hFSH-β-(37–49), modulate the reproductive functions, with the longer peptide being more biologically active. In male or female prepubertal mice, a single injection of 200 μg/g BW ip of hFSH-β-(37–49) or hFSH-β-(34–49) hastened (<i>p</i> < 0.05) puberty, whereas the same treatments given daily for 4 d promoted (<i>p</i> < 0.05) the gonadal steroidogenesis and gamete formation. In addition of either peptide to the in vitro cell cultures, promoted (<i>p</i> < 0.05) the proliferation of primary murine granulosa cells and the estradiol production by upregulating the expression of <i>Ccnd2</i> and <i>Cyp19a1</i>, respectively. In adult female mice, 200 μg/g BW ip of either peptide during diestrus antagonized the FSH-stimulated estradiol increase and uterine weight gain during proestrus. Furthermore, hFSH-β-(34–49) was a more potent (<i>p</i> < 0.05) reproductive modulator than hFSH-β-(37–49), both in vivo and in vitro. We concluded that hFSH-β-(37–49) and especially hFSH-β-(34–49), have the potential for reproductive modulation.https://www.mdpi.com/1422-0067/23/19/11735FSHsynthetic peptidepharmacological agentreproductive modulationmice |
| spellingShingle | Xingfa Han Xinyu Bai Huan Yao Weihao Chen Fengyan Meng Xiaohan Cao Yong Zhuo Lun Hua Guixian Bu Xiaogang Du Qiuxia Liang Xianyin Zeng Two Synthetic Peptides Corresponding to the Human Follicle-Stimulating Hormone β-Subunit Promoted Reproductive Functions in Mice International Journal of Molecular Sciences FSH synthetic peptide pharmacological agent reproductive modulation mice |
| title | Two Synthetic Peptides Corresponding to the Human Follicle-Stimulating Hormone β-Subunit Promoted Reproductive Functions in Mice |
| title_full | Two Synthetic Peptides Corresponding to the Human Follicle-Stimulating Hormone β-Subunit Promoted Reproductive Functions in Mice |
| title_fullStr | Two Synthetic Peptides Corresponding to the Human Follicle-Stimulating Hormone β-Subunit Promoted Reproductive Functions in Mice |
| title_full_unstemmed | Two Synthetic Peptides Corresponding to the Human Follicle-Stimulating Hormone β-Subunit Promoted Reproductive Functions in Mice |
| title_short | Two Synthetic Peptides Corresponding to the Human Follicle-Stimulating Hormone β-Subunit Promoted Reproductive Functions in Mice |
| title_sort | two synthetic peptides corresponding to the human follicle stimulating hormone β subunit promoted reproductive functions in mice |
| topic | FSH synthetic peptide pharmacological agent reproductive modulation mice |
| url | https://www.mdpi.com/1422-0067/23/19/11735 |
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