Assessing the Tsetse Fly Microbiome Composition and the Potential Association of Some Bacteria Taxa with Trypanosome Establishment
The tsetse flies, biological vectors of African trypanosomes, harbour a variety of bacteria involved in their vector competence that may help in developing novel vector control tools. This study provides an inventory of tsetse bacterial communities in Cameroon and explores their possible association...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/6/1141 |
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| author | Calmes Ursain Bouaka Tsakeng Tito Tresor Melachio Tanekou Steve Feudjio Soffack Inaki Tirados Cedrique Noutchih Flobert Njiokou Jude Daiga Bigoga Charles Sinclair Wondji |
| author_facet | Calmes Ursain Bouaka Tsakeng Tito Tresor Melachio Tanekou Steve Feudjio Soffack Inaki Tirados Cedrique Noutchih Flobert Njiokou Jude Daiga Bigoga Charles Sinclair Wondji |
| author_sort | Calmes Ursain Bouaka Tsakeng |
| collection | DOAJ |
| description | The tsetse flies, biological vectors of African trypanosomes, harbour a variety of bacteria involved in their vector competence that may help in developing novel vector control tools. This study provides an inventory of tsetse bacterial communities in Cameroon and explores their possible associations with trypanosome establishment in <i>Glossina palpalis palpalis</i>. High throughput sequencing of the V3-V4 hypervariable region of the bacterial 16S rRNA gene, with subsequent metagenomic, multivariate, and association analyses, were used to investigate the levels and patterns of microbial diversity in four tsetse species. Overall, 31 bacterial genera and four phyla were identified. The primary symbiont <i>Wigglesworthia</i> dominated almost all the samples, with an overall relative abundance of 47.29%, and seemed to be replaced by <i>Serratia</i> or <i>Burkholderia</i> in some <i>G. tachinoides</i> flies. Globally, significant differences were observed in the microbiome diversity and composition among tsetse species and between teneral and non-teneral flies, or between flies displaying or not displaying mature trypanosome infections. In addition, differential abundance testing showed some OTUs, or some bacteria taxa, associated with trypanosome maturation in tsetse flies. These bacteria could be further investigated for an understanding of their mechanism of action and alternatively, transformed and used to block trypanosome development in tsetse flies. |
| first_indexed | 2024-03-09T23:00:20Z |
| format | Article |
| id | doaj.art-b4fccfbabfc74d4a92ad636fcada86ca |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T23:00:20Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-b4fccfbabfc74d4a92ad636fcada86ca2023-11-23T18:03:40ZengMDPI AGMicroorganisms2076-26072022-05-01106114110.3390/microorganisms10061141Assessing the Tsetse Fly Microbiome Composition and the Potential Association of Some Bacteria Taxa with Trypanosome EstablishmentCalmes Ursain Bouaka Tsakeng0Tito Tresor Melachio Tanekou1Steve Feudjio Soffack2Inaki Tirados3Cedrique Noutchih4Flobert Njiokou5Jude Daiga Bigoga6Charles Sinclair Wondji7Centre for Research in Infectious Diseases (CRID), Yaoundé P.O. Box 13591, CameroonCentre for Research in Infectious Diseases (CRID), Yaoundé P.O. Box 13591, CameroonDepartment of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, Yaoundé P.O. Box 812, CameroonDepartment of Vector Biology, Liverpool School of Tropical Medicine Pembroke Place, Liverpool L3 5QA, UKCentre for Research in Infectious Diseases (CRID), Yaoundé P.O. Box 13591, CameroonCentre for Research in Infectious Diseases (CRID), Yaoundé P.O. Box 13591, CameroonDepartment of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé P.O. Box 812, CameroonCentre for Research in Infectious Diseases (CRID), Yaoundé P.O. Box 13591, CameroonThe tsetse flies, biological vectors of African trypanosomes, harbour a variety of bacteria involved in their vector competence that may help in developing novel vector control tools. This study provides an inventory of tsetse bacterial communities in Cameroon and explores their possible associations with trypanosome establishment in <i>Glossina palpalis palpalis</i>. High throughput sequencing of the V3-V4 hypervariable region of the bacterial 16S rRNA gene, with subsequent metagenomic, multivariate, and association analyses, were used to investigate the levels and patterns of microbial diversity in four tsetse species. Overall, 31 bacterial genera and four phyla were identified. The primary symbiont <i>Wigglesworthia</i> dominated almost all the samples, with an overall relative abundance of 47.29%, and seemed to be replaced by <i>Serratia</i> or <i>Burkholderia</i> in some <i>G. tachinoides</i> flies. Globally, significant differences were observed in the microbiome diversity and composition among tsetse species and between teneral and non-teneral flies, or between flies displaying or not displaying mature trypanosome infections. In addition, differential abundance testing showed some OTUs, or some bacteria taxa, associated with trypanosome maturation in tsetse flies. These bacteria could be further investigated for an understanding of their mechanism of action and alternatively, transformed and used to block trypanosome development in tsetse flies.https://www.mdpi.com/2076-2607/10/6/1141tsetse fliestrypanosomesmicrobiomevector competencevector control |
| spellingShingle | Calmes Ursain Bouaka Tsakeng Tito Tresor Melachio Tanekou Steve Feudjio Soffack Inaki Tirados Cedrique Noutchih Flobert Njiokou Jude Daiga Bigoga Charles Sinclair Wondji Assessing the Tsetse Fly Microbiome Composition and the Potential Association of Some Bacteria Taxa with Trypanosome Establishment Microorganisms tsetse flies trypanosomes microbiome vector competence vector control |
| title | Assessing the Tsetse Fly Microbiome Composition and the Potential Association of Some Bacteria Taxa with Trypanosome Establishment |
| title_full | Assessing the Tsetse Fly Microbiome Composition and the Potential Association of Some Bacteria Taxa with Trypanosome Establishment |
| title_fullStr | Assessing the Tsetse Fly Microbiome Composition and the Potential Association of Some Bacteria Taxa with Trypanosome Establishment |
| title_full_unstemmed | Assessing the Tsetse Fly Microbiome Composition and the Potential Association of Some Bacteria Taxa with Trypanosome Establishment |
| title_short | Assessing the Tsetse Fly Microbiome Composition and the Potential Association of Some Bacteria Taxa with Trypanosome Establishment |
| title_sort | assessing the tsetse fly microbiome composition and the potential association of some bacteria taxa with trypanosome establishment |
| topic | tsetse flies trypanosomes microbiome vector competence vector control |
| url | https://www.mdpi.com/2076-2607/10/6/1141 |
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