Protective impact of N-acetyl-L-cysteine on methionine stimulatory effects in rat colon
Introduction: Methionine is an essential sulfuric amino acid, which, according to numerous studies, has a role in the reduction of inflammation, caused by changes in colon, and inhibits important pathways in the development of numerous gastrointestinal disorders. However, subchronic methionine loadi...
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| Format: | Article |
| Language: | English |
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University of Belgrade, Medical Faculty
2016-01-01
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| Series: | Medicinski Podmladak |
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| Online Access: | http://scindeks-clanci.ceon.rs/data/pdf/0369-1527/2016/0369-15271602080H.pdf |
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| author | Hadži-Tanović Lara Todorović Dušan Stojanović Marija Šćepanović Ljiljana Đurić Dragan |
| author_facet | Hadži-Tanović Lara Todorović Dušan Stojanović Marija Šćepanović Ljiljana Đurić Dragan |
| author_sort | Hadži-Tanović Lara |
| collection | DOAJ |
| description | Introduction: Methionine is an essential sulfuric amino acid, which, according to numerous studies, has a role in the reduction of inflammation, caused by changes in colon, and inhibits important pathways in the development of numerous gastrointestinal disorders. However, subchronic methionine loading leads to oxidative stress, which is a potential cause of disease of numerous organs, digestive tract being one of them. N-acetyl-L-cysteine is a natural antioxidant, which affects some of the important signaling pathways and has a potential therapeutic role in treatment of colon disorders. Aim: To examine the influence of N-acetyl-L-cysteine on histological parameters of rat colon, after subchronic methionine load. Material and methods: Experiments were conducted on 15-20 days old male (Wistar albino) rats, weighing 160 g. There were used 24 animals in the experiment, divided into 3 groups, with eight animals per group. First, control group (K), received NaCl, second group received methionine (M group), while third group received methionine + N-acetyl-L-cysteine (N group). The rats were sacrificed after 21 day and tissue samples of colon were obtained. In all of the three groups, changes of histological parameters of rat colon were analyzed. Results: Methionine load leads to statistically significant increase (p < 0.01) in crypt depth of rat colon, lamina muscularis mucosae thickness, the mucosal thickness and increase in lamina propria cell density compared to control, while N-acetyl- L-cysteine application showed statistically significant decrease (p < 0.01) of these histological parameters compared to M group. Conclusion: N-acetyl-L-cysteine has a significantly protective effect on methionine load induced changes in rat colon. |
| first_indexed | 2024-12-14T13:23:15Z |
| format | Article |
| id | doaj.art-b4fedfdae2eb4c479535677b2be692c4 |
| institution | Directory Open Access Journal |
| issn | 0369-1527 2466-5525 |
| language | English |
| last_indexed | 2024-12-14T13:23:15Z |
| publishDate | 2016-01-01 |
| publisher | University of Belgrade, Medical Faculty |
| record_format | Article |
| series | Medicinski Podmladak |
| spelling | doaj.art-b4fedfdae2eb4c479535677b2be692c42022-12-21T22:59:54ZengUniversity of Belgrade, Medical FacultyMedicinski Podmladak0369-15272466-55252016-01-01672808410.5937/medpodm1602080H0369-15271602080HProtective impact of N-acetyl-L-cysteine on methionine stimulatory effects in rat colonHadži-Tanović Lara0Todorović Dušan1Stojanović Marija2Šćepanović Ljiljana3Đurić Dragan4University of Belgrade, Faculty of Medicine, Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, Belgrade, SerbiaMedicinski fakultet, Univerzitet u Beogradu + Institut za medicinsku fiziologiju 'Rihard Burijan'Medicinski fakultet, Univerzitet u Beogradu + Institut za medicinsku fiziologiju 'Rihard Burijan'Medicinski fakultet, Univerzitet u Beogradu + Institut za medicinsku fiziologiju 'Rihard Burijan'Introduction: Methionine is an essential sulfuric amino acid, which, according to numerous studies, has a role in the reduction of inflammation, caused by changes in colon, and inhibits important pathways in the development of numerous gastrointestinal disorders. However, subchronic methionine loading leads to oxidative stress, which is a potential cause of disease of numerous organs, digestive tract being one of them. N-acetyl-L-cysteine is a natural antioxidant, which affects some of the important signaling pathways and has a potential therapeutic role in treatment of colon disorders. Aim: To examine the influence of N-acetyl-L-cysteine on histological parameters of rat colon, after subchronic methionine load. Material and methods: Experiments were conducted on 15-20 days old male (Wistar albino) rats, weighing 160 g. There were used 24 animals in the experiment, divided into 3 groups, with eight animals per group. First, control group (K), received NaCl, second group received methionine (M group), while third group received methionine + N-acetyl-L-cysteine (N group). The rats were sacrificed after 21 day and tissue samples of colon were obtained. In all of the three groups, changes of histological parameters of rat colon were analyzed. Results: Methionine load leads to statistically significant increase (p < 0.01) in crypt depth of rat colon, lamina muscularis mucosae thickness, the mucosal thickness and increase in lamina propria cell density compared to control, while N-acetyl- L-cysteine application showed statistically significant decrease (p < 0.01) of these histological parameters compared to M group. Conclusion: N-acetyl-L-cysteine has a significantly protective effect on methionine load induced changes in rat colon.http://scindeks-clanci.ceon.rs/data/pdf/0369-1527/2016/0369-15271602080H.pdfN-acetyl-L-cysteinemethioninecolonrat |
| spellingShingle | Hadži-Tanović Lara Todorović Dušan Stojanović Marija Šćepanović Ljiljana Đurić Dragan Protective impact of N-acetyl-L-cysteine on methionine stimulatory effects in rat colon Medicinski Podmladak N-acetyl-L-cysteine methionine colon rat |
| title | Protective impact of N-acetyl-L-cysteine on methionine stimulatory effects in rat colon |
| title_full | Protective impact of N-acetyl-L-cysteine on methionine stimulatory effects in rat colon |
| title_fullStr | Protective impact of N-acetyl-L-cysteine on methionine stimulatory effects in rat colon |
| title_full_unstemmed | Protective impact of N-acetyl-L-cysteine on methionine stimulatory effects in rat colon |
| title_short | Protective impact of N-acetyl-L-cysteine on methionine stimulatory effects in rat colon |
| title_sort | protective impact of n acetyl l cysteine on methionine stimulatory effects in rat colon |
| topic | N-acetyl-L-cysteine methionine colon rat |
| url | http://scindeks-clanci.ceon.rs/data/pdf/0369-1527/2016/0369-15271602080H.pdf |
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