Mechanism of anti-cancer effect of β-glucan on SH-SY5Y cell line
Anti-cancer property of fungi derived β-glucan (Lentinula edodes) on several cancer cell lines have been reported. In this work, the SH-SY5Y cell lines were treated with various concentrations of β-glucan (62.5, 125, 250 and 500 μg/mL) and the viability of the cells was tested using the XTT assay af...
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| Format: | Article |
| Language: | English |
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Bangladesh Pharmacological Society
2021-10-01
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| Series: | Bangladesh Journal of Pharmacology |
| Subjects: | |
| Online Access: | https://banglajol.info/index.php/BJP/article/view/54872 |
| _version_ | 1818907142415974400 |
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| author | Ahmet Kemal Filiz Ziad Joha Fatih Yulak |
| author_facet | Ahmet Kemal Filiz Ziad Joha Fatih Yulak |
| author_sort | Ahmet Kemal Filiz |
| collection | DOAJ |
| description | Anti-cancer property of fungi derived β-glucan (Lentinula edodes) on several cancer cell lines have been reported. In this work, the SH-SY5Y cell lines were treated with various concentrations of β-glucan (62.5, 125, 250 and 500 μg/mL) and the viability of the cells was tested using the XTT assay after 24 hours. Cleaved PARP, BCL-2, 8-hydroxy-desoxyguanosine (8-oxo-dG), cleaved caspase 3, Bax, total oxidant, and total antioxidant levels in the cells were measured by commercial kits. β-Glucan significantly decreased the cell viability in SH-SY5Y cells. ELISA tests demonstrated that β-glucan therapy dramatically increased 8-oxo-dG, cleaved caspase 3, Bax, cleaved PARP, total oxidant. However, β-glucan treatment did not change the BCL-2 protein level. Altogether, β-glucan caused significant cytotoxicity in SH-SY5Y cells by inducing oxidative stress, increasing DNA damage, and ultimately increasing apoptosis. |
| first_indexed | 2024-12-19T21:50:25Z |
| format | Article |
| id | doaj.art-b5022bf1a75a4b7d96b5c6bd8ea40fae |
| institution | Directory Open Access Journal |
| issn | 1991-0088 |
| language | English |
| last_indexed | 2024-12-19T21:50:25Z |
| publishDate | 2021-10-01 |
| publisher | Bangladesh Pharmacological Society |
| record_format | Article |
| series | Bangladesh Journal of Pharmacology |
| spelling | doaj.art-b5022bf1a75a4b7d96b5c6bd8ea40fae2022-12-21T20:04:25ZengBangladesh Pharmacological SocietyBangladesh Journal of Pharmacology1991-00882021-10-01164Mechanism of anti-cancer effect of β-glucan on SH-SY5Y cell lineAhmet Kemal Filiz0Ziad Joha1Fatih Yulak2Department of Physiology, School of Medicine, Sivas Cumhuriyet University, Sivas, Turkey. Department of Pharmacology, School of Medicine, Sivas Cumhuriyet University, Sivas, Turkey.Department of Physiology, School of Medicine, Sivas Cumhuriyet University, Sivas, Turkey.Anti-cancer property of fungi derived β-glucan (Lentinula edodes) on several cancer cell lines have been reported. In this work, the SH-SY5Y cell lines were treated with various concentrations of β-glucan (62.5, 125, 250 and 500 μg/mL) and the viability of the cells was tested using the XTT assay after 24 hours. Cleaved PARP, BCL-2, 8-hydroxy-desoxyguanosine (8-oxo-dG), cleaved caspase 3, Bax, total oxidant, and total antioxidant levels in the cells were measured by commercial kits. β-Glucan significantly decreased the cell viability in SH-SY5Y cells. ELISA tests demonstrated that β-glucan therapy dramatically increased 8-oxo-dG, cleaved caspase 3, Bax, cleaved PARP, total oxidant. However, β-glucan treatment did not change the BCL-2 protein level. Altogether, β-glucan caused significant cytotoxicity in SH-SY5Y cells by inducing oxidative stress, increasing DNA damage, and ultimately increasing apoptosis.https://banglajol.info/index.php/BJP/article/view/54872ApoptosisCell viabilityβ-glucanDNA damageELISASH-SY5Y cell |
| spellingShingle | Ahmet Kemal Filiz Ziad Joha Fatih Yulak Mechanism of anti-cancer effect of β-glucan on SH-SY5Y cell line Bangladesh Journal of Pharmacology Apoptosis Cell viability β-glucan DNA damage ELISA SH-SY5Y cell |
| title | Mechanism of anti-cancer effect of β-glucan on SH-SY5Y cell line |
| title_full | Mechanism of anti-cancer effect of β-glucan on SH-SY5Y cell line |
| title_fullStr | Mechanism of anti-cancer effect of β-glucan on SH-SY5Y cell line |
| title_full_unstemmed | Mechanism of anti-cancer effect of β-glucan on SH-SY5Y cell line |
| title_short | Mechanism of anti-cancer effect of β-glucan on SH-SY5Y cell line |
| title_sort | mechanism of anti cancer effect of β glucan on sh sy5y cell line |
| topic | Apoptosis Cell viability β-glucan DNA damage ELISA SH-SY5Y cell |
| url | https://banglajol.info/index.php/BJP/article/view/54872 |
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