Effect of Training Intensity on HIF-1a Gene Expression and Cardiac TG Content in Rats with Non-Alcoholic Fatty Liver Disease
Introduction: HIF-1a is an important regulator in response to hypoxia that affects various genes involved in energy metabolism. The aim of this study was to investigate the effect of exercise intensity on HIF-1a gene expression and cardiac TG content in rats with non-alcoholic fatty liver disease. M...
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| Format: | Article |
| Language: | fas |
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Shahid Sadoughi University of Medical Sciences
2022-10-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| Subjects: | |
| Online Access: | http://jssu.ssu.ac.ir/article-1-5674-en.html |
| _version_ | 1797995805874847744 |
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| author | Mahdi Bagherian Abdolali Banaeifar Sajad Arshadi Mohammad Ali Azarbayjani |
| author_facet | Mahdi Bagherian Abdolali Banaeifar Sajad Arshadi Mohammad Ali Azarbayjani |
| author_sort | Mahdi Bagherian |
| collection | DOAJ |
| description | Introduction: HIF-1a is an important regulator in response to hypoxia that affects various genes involved in energy metabolism. The aim of this study was to investigate the effect of exercise intensity on HIF-1a gene expression and cardiac TG content in rats with non-alcoholic fatty liver disease.
Methods: The present study was performed experimentally on 40 male Wistar rats. Mice were randomly divided into 4 groups. The control groups, low-intensity endurance training and intense interval training, consumed high-fat food for 16 weeks, and then the two training groups participated in exercise for 8 weeks. Furthermore, sham group, which used standard food during this period, at the end of training, the expression level of HIF-1a and the amount of intra-tissue fat of the heart were measured in 4 groups. HIF-1a expression was assessed using real-time PCR and TG assay on an auto-analyzer. SPSS software version 16 and analysis of variance were used to analyze the data.
Results: The results showed that there was a significant difference in the expression of HIF-1a gene between the control and the sham groups, HIIT and LIET groups (P=0.001). Moreover, there was a significant difference in triglyceride between HIIT and LIET groups compared to the sham and control groups, but no significant difference was observed between HIIT and LIET groups (P=0.0001).
Conclusion: The results showed that intense intermittent exercise and low-intensity continuous exercise may reduce cardiac triglyceride content by decreasing HIF-1a expression and increasing fatty acid oxidation. |
| first_indexed | 2024-04-11T10:07:44Z |
| format | Article |
| id | doaj.art-b506b1a02081480792799ea46fa3dc56 |
| institution | Directory Open Access Journal |
| issn | 2228-5741 2228-5733 |
| language | fas |
| last_indexed | 2024-04-11T10:07:44Z |
| publishDate | 2022-10-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| spelling | doaj.art-b506b1a02081480792799ea46fa3dc562022-12-22T04:30:11ZfasShahid Sadoughi University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd2228-57412228-57332022-10-0130750625076Effect of Training Intensity on HIF-1a Gene Expression and Cardiac TG Content in Rats with Non-Alcoholic Fatty Liver DiseaseMahdi Bagherian0Abdolali Banaeifar1Sajad Arshadi2Mohammad Ali Azarbayjani3 Department of Sports Physiology, Islamic Azad University, South Tehran Branch, Tehran, Iran. Department of Sports Physiology, Islamic Azad University, Central Tehran Branch, Tehran, Iran. Department of Sports Physiology, Islamic Azad University, Central Tehran Branch, Tehran, Iran. Department of Sports Physiology, Islamic Azad University, Central Tehran Branch, Tehran, Iran. Introduction: HIF-1a is an important regulator in response to hypoxia that affects various genes involved in energy metabolism. The aim of this study was to investigate the effect of exercise intensity on HIF-1a gene expression and cardiac TG content in rats with non-alcoholic fatty liver disease. Methods: The present study was performed experimentally on 40 male Wistar rats. Mice were randomly divided into 4 groups. The control groups, low-intensity endurance training and intense interval training, consumed high-fat food for 16 weeks, and then the two training groups participated in exercise for 8 weeks. Furthermore, sham group, which used standard food during this period, at the end of training, the expression level of HIF-1a and the amount of intra-tissue fat of the heart were measured in 4 groups. HIF-1a expression was assessed using real-time PCR and TG assay on an auto-analyzer. SPSS software version 16 and analysis of variance were used to analyze the data. Results: The results showed that there was a significant difference in the expression of HIF-1a gene between the control and the sham groups, HIIT and LIET groups (P=0.001). Moreover, there was a significant difference in triglyceride between HIIT and LIET groups compared to the sham and control groups, but no significant difference was observed between HIIT and LIET groups (P=0.0001). Conclusion: The results showed that intense intermittent exercise and low-intensity continuous exercise may reduce cardiac triglyceride content by decreasing HIF-1a expression and increasing fatty acid oxidation.http://jssu.ssu.ac.ir/article-1-5674-en.htmllow intensity endurance traininghigh intensity interval traininghif-1aheart tissuetriglyceride. |
| spellingShingle | Mahdi Bagherian Abdolali Banaeifar Sajad Arshadi Mohammad Ali Azarbayjani Effect of Training Intensity on HIF-1a Gene Expression and Cardiac TG Content in Rats with Non-Alcoholic Fatty Liver Disease Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd low intensity endurance training high intensity interval training hif-1a heart tissue triglyceride. |
| title | Effect of Training Intensity on HIF-1a Gene Expression and Cardiac TG Content in Rats with Non-Alcoholic Fatty Liver Disease |
| title_full | Effect of Training Intensity on HIF-1a Gene Expression and Cardiac TG Content in Rats with Non-Alcoholic Fatty Liver Disease |
| title_fullStr | Effect of Training Intensity on HIF-1a Gene Expression and Cardiac TG Content in Rats with Non-Alcoholic Fatty Liver Disease |
| title_full_unstemmed | Effect of Training Intensity on HIF-1a Gene Expression and Cardiac TG Content in Rats with Non-Alcoholic Fatty Liver Disease |
| title_short | Effect of Training Intensity on HIF-1a Gene Expression and Cardiac TG Content in Rats with Non-Alcoholic Fatty Liver Disease |
| title_sort | effect of training intensity on hif 1a gene expression and cardiac tg content in rats with non alcoholic fatty liver disease |
| topic | low intensity endurance training high intensity interval training hif-1a heart tissue triglyceride. |
| url | http://jssu.ssu.ac.ir/article-1-5674-en.html |
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