Hepatoprotective effect of 2,6-dimethylpyridine N-oxide (Ivin) in experimental model of CCl(4)-induced hepatitis of rats
The effect of concomitant pesticides and plant growth regulators on humans is still not well understood. N-oxide-2,6-dimethylpyridine (Ivin) is the plant growth regulator known to reduce the acute toxicity of pesticides, but its protective mechanisms need to be investigated. The aim of the study was...
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2023-08-01
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| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2023/08/Vasetska_95_4.pdf |
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| author | O. P. Vasetska V. S. Lisovska M. H. Prodanchuk P. H. Zhminko |
| author_facet | O. P. Vasetska V. S. Lisovska M. H. Prodanchuk P. H. Zhminko |
| author_sort | O. P. Vasetska |
| collection | DOAJ |
| description | The effect of concomitant pesticides and plant growth regulators on humans is still not well understood. N-oxide-2,6-dimethylpyridine (Ivin) is the plant growth regulator known to reduce the acute toxicity of pesticides, but its protective mechanisms need to be investigated. The aim of the study was to assess the hepatoprotective ability of 2,6-dimethylpyridine N-oxide (Ivin) using a model of acute CCl4-induced hepatitis. Male Wistar Han rats received two subcutaneous CCl4 injections (0.8 ml/100 g b.w.). Oral Ivin (13 or 0.13 mg/kg) and hepatoprotector “Silybor-35” (5 mg/kg) as reference substances were administered orally one hour pre- and 2 hours post-CCl4 injection. The biochemical assay of blood plasma, estimation of lipid peroxidation products in the liver tissue and histological liver analysis were done. The results of functional tests and histomorphological studies of liver tissue demonstrated that Ivin exhibited a pronounced hepatoprotective effect, more pronounced when it was administered at a low 0.13 mg/kg dose. Calculation of the hepatoprotection efficiency index for Ivin showed that it was comparable to that for “Silybor-35”. |
| first_indexed | 2024-03-11T16:42:37Z |
| format | Article |
| id | doaj.art-b50768e8bfc649cab0c0841ce1490673 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-11T16:42:37Z |
| publishDate | 2023-08-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-b50768e8bfc649cab0c0841ce14906732023-10-23T08:08:49ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032023-08-01954354510.15407/ubj95.04.035Hepatoprotective effect of 2,6-dimethylpyridine N-oxide (Ivin) in experimental model of CCl(4)-induced hepatitis of ratsO. P. Vasetska0https://orcid.org/0000-0002-1919-8593V. S. Lisovska1M. H. Prodanchuk2P. H. Zhminko3SE “L. I. Medved’s Research Center of Preventive Toxicology, Food and Chemical, Ministry of Health, Ukraine”, Kyiv, UkraineSE “L. I. Medved’s Research Center of Preventive Toxicology, Food and Chemical, Ministry of Health, Ukraine”, Kyiv, UkraineSE “L. I. Medved’s Research Center of Preventive Toxicology, Food and Chemical, Ministry of Health, Ukraine”, Kyiv, UkraineSE “L. I. Medved’s Research Center of Preventive Toxicology, Food and Chemical, Ministry of Health, Ukraine”, Kyiv, UkraineThe effect of concomitant pesticides and plant growth regulators on humans is still not well understood. N-oxide-2,6-dimethylpyridine (Ivin) is the plant growth regulator known to reduce the acute toxicity of pesticides, but its protective mechanisms need to be investigated. The aim of the study was to assess the hepatoprotective ability of 2,6-dimethylpyridine N-oxide (Ivin) using a model of acute CCl4-induced hepatitis. Male Wistar Han rats received two subcutaneous CCl4 injections (0.8 ml/100 g b.w.). Oral Ivin (13 or 0.13 mg/kg) and hepatoprotector “Silybor-35” (5 mg/kg) as reference substances were administered orally one hour pre- and 2 hours post-CCl4 injection. The biochemical assay of blood plasma, estimation of lipid peroxidation products in the liver tissue and histological liver analysis were done. The results of functional tests and histomorphological studies of liver tissue demonstrated that Ivin exhibited a pronounced hepatoprotective effect, more pronounced when it was administered at a low 0.13 mg/kg dose. Calculation of the hepatoprotection efficiency index for Ivin showed that it was comparable to that for “Silybor-35”.http://ukrbiochemjournal.org/wp-content/uploads/2023/08/Vasetska_95_4.pdfepatoprotective effectivinсcl4-induced hepatitis of rats |
| spellingShingle | O. P. Vasetska V. S. Lisovska M. H. Prodanchuk P. H. Zhminko Hepatoprotective effect of 2,6-dimethylpyridine N-oxide (Ivin) in experimental model of CCl(4)-induced hepatitis of rats The Ukrainian Biochemical Journal epatoprotective effect ivin сcl4-induced hepatitis of rats |
| title | Hepatoprotective effect of 2,6-dimethylpyridine N-oxide (Ivin) in experimental model of CCl(4)-induced hepatitis of rats |
| title_full | Hepatoprotective effect of 2,6-dimethylpyridine N-oxide (Ivin) in experimental model of CCl(4)-induced hepatitis of rats |
| title_fullStr | Hepatoprotective effect of 2,6-dimethylpyridine N-oxide (Ivin) in experimental model of CCl(4)-induced hepatitis of rats |
| title_full_unstemmed | Hepatoprotective effect of 2,6-dimethylpyridine N-oxide (Ivin) in experimental model of CCl(4)-induced hepatitis of rats |
| title_short | Hepatoprotective effect of 2,6-dimethylpyridine N-oxide (Ivin) in experimental model of CCl(4)-induced hepatitis of rats |
| title_sort | hepatoprotective effect of 2 6 dimethylpyridine n oxide ivin in experimental model of ccl 4 induced hepatitis of rats |
| topic | epatoprotective effect ivin сcl4-induced hepatitis of rats |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2023/08/Vasetska_95_4.pdf |
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