Evaluation of artificial drug incorporation into hair for the production of quality control samples
Hair analysis is thoroughly used in forensic toxicology although there are still some points of concern such as lack of reference material, whether for internal quality controls (IQC) or even for analytical validation process verification. Nonetheless spiked samples are still widely used, mainly for...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal do Rio Grande do Sul
2021-12-01
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| Series: | Drug Analytical Research |
| Online Access: | https://seer.ufrgs.br/index.php/produto&producao/ojs/include/index.php/dar/article/view/118887 |
| _version_ | 1797388953843662848 |
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| author | Flavia Lopes Roveri Ana Miguel Fonseca Pego Sarah Eller Tiago Franco de Oliveira Mauricio Yonamine |
| author_facet | Flavia Lopes Roveri Ana Miguel Fonseca Pego Sarah Eller Tiago Franco de Oliveira Mauricio Yonamine |
| author_sort | Flavia Lopes Roveri |
| collection | DOAJ |
| description | Hair analysis is thoroughly used in forensic toxicology although there are still some points of concern such as lack of reference material, whether for internal quality controls (IQC) or even for analytical validation process verification. Nonetheless spiked samples are still widely used, mainly for method development, it is not possible to evaluate its actual reproducibility and accuracy. As an alternative, IQC could be produced artificially by the laboratories themselves. The aim of this work was the evaluation of the phenomenon of artificial incorporation of drugs into hair to produce internal quality controls. These controls have been prepared according to the recommendations from the National Institute of Standards and Technology. For the amphetamine group, amphetamine and MDMA, showed different incorporation rates, of 0.17 up to 0.5% for amphetamine and 0.10 up to 0.4% for MDMA. As for cocaine, the incorporation rate was progressive over the course of days, ranging from 0.15 to 0.75%. The highest incorporation was found for diazepam, from 0.57%. to 3.75%. Lower rates were obtained for morphine, ranging from 0.08 to 0.25%, given that the incorporation rate of 0.25% has been reached on the ninth day. Some factors such as incubation time, agitation process and sample washing probably influenced the way analytes incorporate into the matrix including the effects of homogenization of the samples. Overall, knowing the incorporation profile of each analyte it is possible to produce IQC, with different concentrations. Thus, laboratories will have the fortified samples as a better tool for evaluating their own methodology.
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| first_indexed | 2024-03-08T22:48:31Z |
| format | Article |
| id | doaj.art-b5096b84cc704d8590db9f39cd388b82 |
| institution | Directory Open Access Journal |
| issn | 2527-2616 |
| language | English |
| last_indexed | 2024-03-08T22:48:31Z |
| publishDate | 2021-12-01 |
| publisher | Universidade Federal do Rio Grande do Sul |
| record_format | Article |
| series | Drug Analytical Research |
| spelling | doaj.art-b5096b84cc704d8590db9f39cd388b822023-12-16T18:13:20ZengUniversidade Federal do Rio Grande do SulDrug Analytical Research2527-26162021-12-0152Evaluation of artificial drug incorporation into hair for the production of quality control samplesFlavia Lopes Roveri0Ana Miguel Fonseca Pego1Sarah Eller2Tiago Franco de Oliveira3Mauricio Yonamine4School of Pharmaceutical Sciences, University of São PauloSchool of Pharmaceutical Sciences, University of São PauloUniversity of Health Sciences of Porto AlegreUniversity of Health Sciences of Porto AlegreSchool of Pharmaceutical Sciences, University of São PauloHair analysis is thoroughly used in forensic toxicology although there are still some points of concern such as lack of reference material, whether for internal quality controls (IQC) or even for analytical validation process verification. Nonetheless spiked samples are still widely used, mainly for method development, it is not possible to evaluate its actual reproducibility and accuracy. As an alternative, IQC could be produced artificially by the laboratories themselves. The aim of this work was the evaluation of the phenomenon of artificial incorporation of drugs into hair to produce internal quality controls. These controls have been prepared according to the recommendations from the National Institute of Standards and Technology. For the amphetamine group, amphetamine and MDMA, showed different incorporation rates, of 0.17 up to 0.5% for amphetamine and 0.10 up to 0.4% for MDMA. As for cocaine, the incorporation rate was progressive over the course of days, ranging from 0.15 to 0.75%. The highest incorporation was found for diazepam, from 0.57%. to 3.75%. Lower rates were obtained for morphine, ranging from 0.08 to 0.25%, given that the incorporation rate of 0.25% has been reached on the ninth day. Some factors such as incubation time, agitation process and sample washing probably influenced the way analytes incorporate into the matrix including the effects of homogenization of the samples. Overall, knowing the incorporation profile of each analyte it is possible to produce IQC, with different concentrations. Thus, laboratories will have the fortified samples as a better tool for evaluating their own methodology. https://seer.ufrgs.br/index.php/produto&producao/ojs/include/index.php/dar/article/view/118887 |
| spellingShingle | Flavia Lopes Roveri Ana Miguel Fonseca Pego Sarah Eller Tiago Franco de Oliveira Mauricio Yonamine Evaluation of artificial drug incorporation into hair for the production of quality control samples Drug Analytical Research |
| title | Evaluation of artificial drug incorporation into hair for the production of quality control samples |
| title_full | Evaluation of artificial drug incorporation into hair for the production of quality control samples |
| title_fullStr | Evaluation of artificial drug incorporation into hair for the production of quality control samples |
| title_full_unstemmed | Evaluation of artificial drug incorporation into hair for the production of quality control samples |
| title_short | Evaluation of artificial drug incorporation into hair for the production of quality control samples |
| title_sort | evaluation of artificial drug incorporation into hair for the production of quality control samples |
| url | https://seer.ufrgs.br/index.php/produto&producao/ojs/include/index.php/dar/article/view/118887 |
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