Expanding xylose metabolism in yeast for plant cell wall conversion to biofuels
Sustainable biofuel production from renewable biomass will require the efficient and complete use of all abundant sugars in the plant cell wall. Using the cellulolytic fungus Neurospora crassa as a model, we identified a xylodextrin transport and consumption pathway required for its growth on hemice...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2015-02-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/05896 |
| _version_ | 1797997495274438656 |
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| author | Xin Li Vivian Yaci Yu Yuping Lin Kulika Chomvong Raíssa Estrela Annsea Park Julie M Liang Elizabeth A Znameroski Joanna Feehan Soo Rin Kim Yong-Su Jin N Louise Glass Jamie HD Cate |
| author_facet | Xin Li Vivian Yaci Yu Yuping Lin Kulika Chomvong Raíssa Estrela Annsea Park Julie M Liang Elizabeth A Znameroski Joanna Feehan Soo Rin Kim Yong-Su Jin N Louise Glass Jamie HD Cate |
| author_sort | Xin Li |
| collection | DOAJ |
| description | Sustainable biofuel production from renewable biomass will require the efficient and complete use of all abundant sugars in the plant cell wall. Using the cellulolytic fungus Neurospora crassa as a model, we identified a xylodextrin transport and consumption pathway required for its growth on hemicellulose. Reconstitution of this xylodextrin utilization pathway in Saccharomyces cerevisiae revealed that fungal xylose reductases act as xylodextrin reductases, producing xylosyl-xylitol oligomers as metabolic intermediates. These xylosyl-xylitol intermediates are generated by diverse fungi and bacteria, indicating that xylodextrin reduction is widespread in nature. Xylodextrins and xylosyl-xylitol oligomers are then hydrolyzed by two hydrolases to generate intracellular xylose and xylitol. Xylodextrin consumption using a xylodextrin transporter, xylodextrin reductases and tandem intracellular hydrolases in cofermentations with sucrose and glucose greatly expands the capacity of yeast to use plant cell wall-derived sugars and has the potential to increase the efficiency of both first-generation and next-generation biofuel production. |
| first_indexed | 2024-04-11T10:33:55Z |
| format | Article |
| id | doaj.art-b509c1018164456e97266f9481d95d41 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T10:33:55Z |
| publishDate | 2015-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-b509c1018164456e97266f9481d95d412022-12-22T04:29:21ZengeLife Sciences Publications LtdeLife2050-084X2015-02-01410.7554/eLife.05896Expanding xylose metabolism in yeast for plant cell wall conversion to biofuelsXin Li0Vivian Yaci Yu1Yuping Lin2Kulika Chomvong3Raíssa Estrela4Annsea Park5Julie M Liang6Elizabeth A Znameroski7Joanna Feehan8Soo Rin Kim9Yong-Su Jin10N Louise Glass11Jamie HD Cate12Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States; Impossible Foods, Inc, Redwood City, United StatesDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Chemistry, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesInstitute for Genomic Biology, University of Illinois, Urbana, United States; School of Food Science and Biotechnology, Kyungpook National University, Daegu, Republic of KoreaInstitute for Genomic Biology, University of Illinois, Urbana, United States; Department of Food Science and Human Nutrition, University of Illinois, Urbana, United StatesDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States; Department of Chemistry, University of California, Berkeley, Berkeley, United States; Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, United StatesSustainable biofuel production from renewable biomass will require the efficient and complete use of all abundant sugars in the plant cell wall. Using the cellulolytic fungus Neurospora crassa as a model, we identified a xylodextrin transport and consumption pathway required for its growth on hemicellulose. Reconstitution of this xylodextrin utilization pathway in Saccharomyces cerevisiae revealed that fungal xylose reductases act as xylodextrin reductases, producing xylosyl-xylitol oligomers as metabolic intermediates. These xylosyl-xylitol intermediates are generated by diverse fungi and bacteria, indicating that xylodextrin reduction is widespread in nature. Xylodextrins and xylosyl-xylitol oligomers are then hydrolyzed by two hydrolases to generate intracellular xylose and xylitol. Xylodextrin consumption using a xylodextrin transporter, xylodextrin reductases and tandem intracellular hydrolases in cofermentations with sucrose and glucose greatly expands the capacity of yeast to use plant cell wall-derived sugars and has the potential to increase the efficiency of both first-generation and next-generation biofuel production.https://elifesciences.org/articles/05896xylosehemicellulosebiofuelxylodextrincofermentationxylosyl-xylitol |
| spellingShingle | Xin Li Vivian Yaci Yu Yuping Lin Kulika Chomvong Raíssa Estrela Annsea Park Julie M Liang Elizabeth A Znameroski Joanna Feehan Soo Rin Kim Yong-Su Jin N Louise Glass Jamie HD Cate Expanding xylose metabolism in yeast for plant cell wall conversion to biofuels eLife xylose hemicellulose biofuel xylodextrin cofermentation xylosyl-xylitol |
| title | Expanding xylose metabolism in yeast for plant cell wall conversion to biofuels |
| title_full | Expanding xylose metabolism in yeast for plant cell wall conversion to biofuels |
| title_fullStr | Expanding xylose metabolism in yeast for plant cell wall conversion to biofuels |
| title_full_unstemmed | Expanding xylose metabolism in yeast for plant cell wall conversion to biofuels |
| title_short | Expanding xylose metabolism in yeast for plant cell wall conversion to biofuels |
| title_sort | expanding xylose metabolism in yeast for plant cell wall conversion to biofuels |
| topic | xylose hemicellulose biofuel xylodextrin cofermentation xylosyl-xylitol |
| url | https://elifesciences.org/articles/05896 |
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