Isolation and Genome-Based Characterization of Biocontrol Potential of <i>Bacillus siamensis</i> YB-1631 against Wheat Crown Rot Caused by <i>Fusarium pseudograminearum</i>
Fusarium crown rot (FCR) caused by <i>Fusarium pseudograminearum</i> is one of the most serious soil-borne diseases of wheat. Among 58 bacterial isolates from the rhizosphere soil of winter wheat seedlings, strain YB-1631 was found to have the highest in vitro antagonism to <i>F. p...
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MDPI AG
2023-05-01
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| Online Access: | https://www.mdpi.com/2309-608X/9/5/547 |
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| author | Qianqian Dong Qingxiang Liu Paul H. Goodwin Xiaoxu Deng Wen Xu Mingcong Xia Jie Zhang Runhong Sun Chao Wu Qi Wang Kun Wu Lirong Yang |
| author_facet | Qianqian Dong Qingxiang Liu Paul H. Goodwin Xiaoxu Deng Wen Xu Mingcong Xia Jie Zhang Runhong Sun Chao Wu Qi Wang Kun Wu Lirong Yang |
| author_sort | Qianqian Dong |
| collection | DOAJ |
| description | Fusarium crown rot (FCR) caused by <i>Fusarium pseudograminearum</i> is one of the most serious soil-borne diseases of wheat. Among 58 bacterial isolates from the rhizosphere soil of winter wheat seedlings, strain YB-1631 was found to have the highest in vitro antagonism to <i>F. pseudograminearum</i> growth. LB cell-free culture filtrates inhibited mycelial growth and conidia germination of <i>F. pseudograminearum</i> by 84.14% and 92.23%, respectively. The culture filtrate caused distortion and disruption of the cells. Using a face-to-face plate assay, volatile substances produced by YB-1631 inhibited <i>F. pseudograminearum</i> growth by 68.16%. In the greenhouse, YB-1631 reduced the incidence of FCR on wheat seedlings by 84.02% and increased root and shoot fresh weights by 20.94% and 9.63%, respectively. YB-1631 was identified as <i>Bacillus siamensis</i> based on the <i>gyrB</i> sequence and average nucleotide identity of the complete genome. The complete genome was 4,090,312 bp with 4357 genes and 45.92% GC content. In the genome, genes were identified for root colonization, including those for chemotaxis and biofilm production, genes for plant growth promotion, including those for phytohormones and nutrient assimilation, and genes for biocontrol activity, including those for siderophores, extracellular hydrolase, volatiles, nonribosomal peptides, polyketide antibiotics, and elicitors of induced systemic resistance. In vitro production of siderophore, β-1, 3-glucanase, amylase, protease, cellulase, phosphorus solubilization, and indole acetic acid were detected. <i>Bacillus siamensis</i> YB-1631 appears to have significant potential in promoting wheat growth and controlling wheat FCR caused by <i>F. pseudograminearum</i>. |
| first_indexed | 2024-03-11T03:35:47Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-11T03:35:47Z |
| publishDate | 2023-05-01 |
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| spelling | doaj.art-b509ce5a934b45f2a69c99fb37ebee792023-11-18T02:02:07ZengMDPI AGJournal of Fungi2309-608X2023-05-019554710.3390/jof9050547Isolation and Genome-Based Characterization of Biocontrol Potential of <i>Bacillus siamensis</i> YB-1631 against Wheat Crown Rot Caused by <i>Fusarium pseudograminearum</i>Qianqian Dong0Qingxiang Liu1Paul H. Goodwin2Xiaoxu Deng3Wen Xu4Mingcong Xia5Jie Zhang6Runhong Sun7Chao Wu8Qi Wang9Kun Wu10Lirong Yang11College of Life Sciences, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Life Sciences, Henan Agricultural University, Zhengzhou 450046, ChinaSchool of Environmental Sciences, University of Guelph, Guelph, ON N1G 2W1, CanadaInstitute of Plant Protection Research, Henan Academy of Agricultural Sciences, Henan Agricultural Microbiology Innovation Center, Zhengzhou 450002, ChinaInstitute of Plant Protection Research, Henan Academy of Agricultural Sciences, Henan Agricultural Microbiology Innovation Center, Zhengzhou 450002, ChinaInstitute of Plant Protection Research, Henan Academy of Agricultural Sciences, Henan Agricultural Microbiology Innovation Center, Zhengzhou 450002, ChinaInstitute of Plant Protection Research, Henan Academy of Agricultural Sciences, Henan Agricultural Microbiology Innovation Center, Zhengzhou 450002, ChinaInstitute of Plant Protection Research, Henan Academy of Agricultural Sciences, Henan Agricultural Microbiology Innovation Center, Zhengzhou 450002, ChinaInstitute of Plant Protection Research, Henan Academy of Agricultural Sciences, Henan Agricultural Microbiology Innovation Center, Zhengzhou 450002, ChinaCollege of Plant Protection, China Agricultural University, Beijing 100193, ChinaCollege of Life Sciences, Henan Agricultural University, Zhengzhou 450046, ChinaInstitute of Plant Protection Research, Henan Academy of Agricultural Sciences, Henan Agricultural Microbiology Innovation Center, Zhengzhou 450002, ChinaFusarium crown rot (FCR) caused by <i>Fusarium pseudograminearum</i> is one of the most serious soil-borne diseases of wheat. Among 58 bacterial isolates from the rhizosphere soil of winter wheat seedlings, strain YB-1631 was found to have the highest in vitro antagonism to <i>F. pseudograminearum</i> growth. LB cell-free culture filtrates inhibited mycelial growth and conidia germination of <i>F. pseudograminearum</i> by 84.14% and 92.23%, respectively. The culture filtrate caused distortion and disruption of the cells. Using a face-to-face plate assay, volatile substances produced by YB-1631 inhibited <i>F. pseudograminearum</i> growth by 68.16%. In the greenhouse, YB-1631 reduced the incidence of FCR on wheat seedlings by 84.02% and increased root and shoot fresh weights by 20.94% and 9.63%, respectively. YB-1631 was identified as <i>Bacillus siamensis</i> based on the <i>gyrB</i> sequence and average nucleotide identity of the complete genome. The complete genome was 4,090,312 bp with 4357 genes and 45.92% GC content. In the genome, genes were identified for root colonization, including those for chemotaxis and biofilm production, genes for plant growth promotion, including those for phytohormones and nutrient assimilation, and genes for biocontrol activity, including those for siderophores, extracellular hydrolase, volatiles, nonribosomal peptides, polyketide antibiotics, and elicitors of induced systemic resistance. In vitro production of siderophore, β-1, 3-glucanase, amylase, protease, cellulase, phosphorus solubilization, and indole acetic acid were detected. <i>Bacillus siamensis</i> YB-1631 appears to have significant potential in promoting wheat growth and controlling wheat FCR caused by <i>F. pseudograminearum</i>.https://www.mdpi.com/2309-608X/9/5/547Fusarium crown rot<i>Bacillus siamensi</i>s<i>Fusarium pseudograminearum</i>biocontrolgenomegrowth promotion |
| spellingShingle | Qianqian Dong Qingxiang Liu Paul H. Goodwin Xiaoxu Deng Wen Xu Mingcong Xia Jie Zhang Runhong Sun Chao Wu Qi Wang Kun Wu Lirong Yang Isolation and Genome-Based Characterization of Biocontrol Potential of <i>Bacillus siamensis</i> YB-1631 against Wheat Crown Rot Caused by <i>Fusarium pseudograminearum</i> Journal of Fungi Fusarium crown rot <i>Bacillus siamensi</i>s <i>Fusarium pseudograminearum</i> biocontrol genome growth promotion |
| title | Isolation and Genome-Based Characterization of Biocontrol Potential of <i>Bacillus siamensis</i> YB-1631 against Wheat Crown Rot Caused by <i>Fusarium pseudograminearum</i> |
| title_full | Isolation and Genome-Based Characterization of Biocontrol Potential of <i>Bacillus siamensis</i> YB-1631 against Wheat Crown Rot Caused by <i>Fusarium pseudograminearum</i> |
| title_fullStr | Isolation and Genome-Based Characterization of Biocontrol Potential of <i>Bacillus siamensis</i> YB-1631 against Wheat Crown Rot Caused by <i>Fusarium pseudograminearum</i> |
| title_full_unstemmed | Isolation and Genome-Based Characterization of Biocontrol Potential of <i>Bacillus siamensis</i> YB-1631 against Wheat Crown Rot Caused by <i>Fusarium pseudograminearum</i> |
| title_short | Isolation and Genome-Based Characterization of Biocontrol Potential of <i>Bacillus siamensis</i> YB-1631 against Wheat Crown Rot Caused by <i>Fusarium pseudograminearum</i> |
| title_sort | isolation and genome based characterization of biocontrol potential of i bacillus siamensis i yb 1631 against wheat crown rot caused by i fusarium pseudograminearum i |
| topic | Fusarium crown rot <i>Bacillus siamensi</i>s <i>Fusarium pseudograminearum</i> biocontrol genome growth promotion |
| url | https://www.mdpi.com/2309-608X/9/5/547 |
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