Flame resistant cotton lines generated by synergistic epistasis in a MAGIC population.
Textiles made from cotton fibers are flammable and thus often include flame retardant additives for consumer safety. Transgressive segregation in multi-parent populations facilitates new combinations of alleles of genes and can result in traits that are superior to those of any of the parents. A scr...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0278696 |
| _version_ | 1827998767447539712 |
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| author | Gregory N Thyssen Brian D Condon Doug J Hinchliffe Linghe Zeng Marina Naoumkina Johnie N Jenkins Jack C McCarty Ruixiu Sui Crista Madison Ping Li David D Fang |
| author_facet | Gregory N Thyssen Brian D Condon Doug J Hinchliffe Linghe Zeng Marina Naoumkina Johnie N Jenkins Jack C McCarty Ruixiu Sui Crista Madison Ping Li David D Fang |
| author_sort | Gregory N Thyssen |
| collection | DOAJ |
| description | Textiles made from cotton fibers are flammable and thus often include flame retardant additives for consumer safety. Transgressive segregation in multi-parent populations facilitates new combinations of alleles of genes and can result in traits that are superior to those of any of the parents. A screen of 257 recombinant inbred lines from a multi-parent advanced generation intercross (MAGIC) population for naturally enhance flame retardance (FR) was conducted. All eleven parents, like all conventional white fiber cotton cultivars produce flammable fabric. MAGIC recombinant inbred lines (RILs) that produced fibers with significantly lower heat release capacities (HRC) as measured by microscale combustion calorimetry (MCC) were identified and the stability of the phenotypes of the outliers were confirmed when the RILs were grown at an additional location. Of the textiles fabricated from the five superior RILs, four exhibited the novel characteristic of inherent flame resistance. When exposed to open flame by standard 45° incline flammability testing, these four fabrics self-extinguished. To determine the genetic architecture of this novel trait, linkage, epistatic and multi-locus genome wide association studies (GWAS) were conducted with 473k SNPs identified by whole genome sequencing (WGS). Transcriptomes of developing fiber cells from select RILs were sequenced (RNAseq). Together, these data provide insight into the genetic mechanism of the unexpected emergence of flame-resistant cotton by transgressive segregation in a breeding program. The incorporation of this trait into global cotton germplasm by breeding has the potential to greatly reduce the costs and impacts of flame-retardant chemicals. |
| first_indexed | 2024-04-10T05:48:54Z |
| format | Article |
| id | doaj.art-b510e002c1684310959d97337043c973 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-10T05:48:54Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b510e002c1684310959d97337043c9732023-03-05T05:31:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01181e027869610.1371/journal.pone.0278696Flame resistant cotton lines generated by synergistic epistasis in a MAGIC population.Gregory N ThyssenBrian D CondonDoug J HinchliffeLinghe ZengMarina NaoumkinaJohnie N JenkinsJack C McCartyRuixiu SuiCrista MadisonPing LiDavid D FangTextiles made from cotton fibers are flammable and thus often include flame retardant additives for consumer safety. Transgressive segregation in multi-parent populations facilitates new combinations of alleles of genes and can result in traits that are superior to those of any of the parents. A screen of 257 recombinant inbred lines from a multi-parent advanced generation intercross (MAGIC) population for naturally enhance flame retardance (FR) was conducted. All eleven parents, like all conventional white fiber cotton cultivars produce flammable fabric. MAGIC recombinant inbred lines (RILs) that produced fibers with significantly lower heat release capacities (HRC) as measured by microscale combustion calorimetry (MCC) were identified and the stability of the phenotypes of the outliers were confirmed when the RILs were grown at an additional location. Of the textiles fabricated from the five superior RILs, four exhibited the novel characteristic of inherent flame resistance. When exposed to open flame by standard 45° incline flammability testing, these four fabrics self-extinguished. To determine the genetic architecture of this novel trait, linkage, epistatic and multi-locus genome wide association studies (GWAS) were conducted with 473k SNPs identified by whole genome sequencing (WGS). Transcriptomes of developing fiber cells from select RILs were sequenced (RNAseq). Together, these data provide insight into the genetic mechanism of the unexpected emergence of flame-resistant cotton by transgressive segregation in a breeding program. The incorporation of this trait into global cotton germplasm by breeding has the potential to greatly reduce the costs and impacts of flame-retardant chemicals.https://doi.org/10.1371/journal.pone.0278696 |
| spellingShingle | Gregory N Thyssen Brian D Condon Doug J Hinchliffe Linghe Zeng Marina Naoumkina Johnie N Jenkins Jack C McCarty Ruixiu Sui Crista Madison Ping Li David D Fang Flame resistant cotton lines generated by synergistic epistasis in a MAGIC population. PLoS ONE |
| title | Flame resistant cotton lines generated by synergistic epistasis in a MAGIC population. |
| title_full | Flame resistant cotton lines generated by synergistic epistasis in a MAGIC population. |
| title_fullStr | Flame resistant cotton lines generated by synergistic epistasis in a MAGIC population. |
| title_full_unstemmed | Flame resistant cotton lines generated by synergistic epistasis in a MAGIC population. |
| title_short | Flame resistant cotton lines generated by synergistic epistasis in a MAGIC population. |
| title_sort | flame resistant cotton lines generated by synergistic epistasis in a magic population |
| url | https://doi.org/10.1371/journal.pone.0278696 |
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