Roles of Process Parameters on the Ricinoleic Acid Production from Castor Oil by <i>Aspergillus flavus</i> BU22S
Ricinoleic acid is a biobased green chemical industrially produced from castor oil. Microbial conversion is a cleaner and greener approach to ricinoleic acid production from castor oil. These processes should be further optimized for a better yield of the product. <i>Aspergillus flavus</i&g...
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MDPI AG
2023-03-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/4/318 |
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| author | Shikha Singh Sumit Sharma Saurabh Jyoti Sarma Satinder Kaur Brar |
| author_facet | Shikha Singh Sumit Sharma Saurabh Jyoti Sarma Satinder Kaur Brar |
| author_sort | Shikha Singh |
| collection | DOAJ |
| description | Ricinoleic acid is a biobased green chemical industrially produced from castor oil. Microbial conversion is a cleaner and greener approach to ricinoleic acid production from castor oil. These processes should be further optimized for a better yield of the product. <i>Aspergillus flavus</i> BU22S was used to convert castor oil into ricinoleic acid. The strain was isolated and identified by molecular biological techniques. It was found to be effective in the biotransformation of castor oil. The ricinoleic acid production and dry cell weight of the fungus were studied as functions of time. In this study, to increase the yield of ricinoleic acid and decrease the oil loss, which microorganisms utilizes in biomass production, response surface methodology (RSM) has been used for process optimization. The central composite design was used to optimize the predictor variables such as oil concentration (% <i>w/v</i>), glucose concentration (% <i>w/v</i>), and calcium chloride concentration (% <i>w/v</i>) to increase the overall yield of ricinoleic acid. A quadratic model was found to be the best fit to predict the responses of the experimental results. The model suggested that the concentrations of oil, glucose, and calcium chloride should be lower in order to increase the ricinoleic acid yield and minimize the oil loss. The bench scale studies of optimized conditions from RSM were also conducted. The yield of ricinoleic acid in batch and fed-batch culture studies was also compared. The yield of the ricinoleic acid in batch culture was 21.67 g/kg of total oil. The yield of ricinoleic acid in fed-batch culture in the absence of an external air supply was 46.77 g/kg of total oil. In this case, the oil loss was also reduced to only 12%. |
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| language | English |
| last_indexed | 2024-03-11T05:02:24Z |
| publishDate | 2023-03-01 |
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| series | Fermentation |
| spelling | doaj.art-b510ea3c8f6e4c3694c521f98a32a3732023-11-17T19:10:10ZengMDPI AGFermentation2311-56372023-03-019431810.3390/fermentation9040318Roles of Process Parameters on the Ricinoleic Acid Production from Castor Oil by <i>Aspergillus flavus</i> BU22SShikha Singh0Sumit Sharma1Saurabh Jyoti Sarma2Satinder Kaur Brar3Department of Biotechnology, Bennett University, Greater Noida 201310, IndiaDepartment of Biotechnology, Bennett University, Greater Noida 201310, IndiaDepartment of Biotechnology, Bennett University, Greater Noida 201310, IndiaLassonde School of Engineering, York University, North York, Toronto, ON M3J1P3, CanadaRicinoleic acid is a biobased green chemical industrially produced from castor oil. Microbial conversion is a cleaner and greener approach to ricinoleic acid production from castor oil. These processes should be further optimized for a better yield of the product. <i>Aspergillus flavus</i> BU22S was used to convert castor oil into ricinoleic acid. The strain was isolated and identified by molecular biological techniques. It was found to be effective in the biotransformation of castor oil. The ricinoleic acid production and dry cell weight of the fungus were studied as functions of time. In this study, to increase the yield of ricinoleic acid and decrease the oil loss, which microorganisms utilizes in biomass production, response surface methodology (RSM) has been used for process optimization. The central composite design was used to optimize the predictor variables such as oil concentration (% <i>w/v</i>), glucose concentration (% <i>w/v</i>), and calcium chloride concentration (% <i>w/v</i>) to increase the overall yield of ricinoleic acid. A quadratic model was found to be the best fit to predict the responses of the experimental results. The model suggested that the concentrations of oil, glucose, and calcium chloride should be lower in order to increase the ricinoleic acid yield and minimize the oil loss. The bench scale studies of optimized conditions from RSM were also conducted. The yield of ricinoleic acid in batch and fed-batch culture studies was also compared. The yield of the ricinoleic acid in batch culture was 21.67 g/kg of total oil. The yield of ricinoleic acid in fed-batch culture in the absence of an external air supply was 46.77 g/kg of total oil. In this case, the oil loss was also reduced to only 12%.https://www.mdpi.com/2311-5637/9/4/318<i>Aspergillus flavus</i>calcium chloridecastor oilricinoleic acidRSM |
| spellingShingle | Shikha Singh Sumit Sharma Saurabh Jyoti Sarma Satinder Kaur Brar Roles of Process Parameters on the Ricinoleic Acid Production from Castor Oil by <i>Aspergillus flavus</i> BU22S Fermentation <i>Aspergillus flavus</i> calcium chloride castor oil ricinoleic acid RSM |
| title | Roles of Process Parameters on the Ricinoleic Acid Production from Castor Oil by <i>Aspergillus flavus</i> BU22S |
| title_full | Roles of Process Parameters on the Ricinoleic Acid Production from Castor Oil by <i>Aspergillus flavus</i> BU22S |
| title_fullStr | Roles of Process Parameters on the Ricinoleic Acid Production from Castor Oil by <i>Aspergillus flavus</i> BU22S |
| title_full_unstemmed | Roles of Process Parameters on the Ricinoleic Acid Production from Castor Oil by <i>Aspergillus flavus</i> BU22S |
| title_short | Roles of Process Parameters on the Ricinoleic Acid Production from Castor Oil by <i>Aspergillus flavus</i> BU22S |
| title_sort | roles of process parameters on the ricinoleic acid production from castor oil by i aspergillus flavus i bu22s |
| topic | <i>Aspergillus flavus</i> calcium chloride castor oil ricinoleic acid RSM |
| url | https://www.mdpi.com/2311-5637/9/4/318 |
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