Functional Characterization of Target of Rapamycin Signaling in Verticillium dahliae
More than 200 plants have been suffering from Verticillium wilt caused by Verticillium dahliae (V. dahliae) across the world. The target of rapamycin (TOR) is a lethal gene and controls cell growth and development in various eukaryotes, but little is known about TOR signaling in V. dahliae. Here, we...
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| Format: | Article |
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Frontiers Media S.A.
2019-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.00501/full |
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| author | Linxuan Li Tingting Zhu Yun Song Yun Song Xiumei Luo Li Feng Fengping Zhuo Fengping Zhuo Fuguang Li Fuguang Li Maozhi Ren |
| author_facet | Linxuan Li Tingting Zhu Yun Song Yun Song Xiumei Luo Li Feng Fengping Zhuo Fengping Zhuo Fuguang Li Fuguang Li Maozhi Ren |
| author_sort | Linxuan Li |
| collection | DOAJ |
| description | More than 200 plants have been suffering from Verticillium wilt caused by Verticillium dahliae (V. dahliae) across the world. The target of rapamycin (TOR) is a lethal gene and controls cell growth and development in various eukaryotes, but little is known about TOR signaling in V. dahliae. Here, we found that V. dahliae strain is hypersensitive to rapamycin in the presence of rapamycin binding protein VdFKBP12 while the deletion mutant aaavdfkbp12 is insensitive to rapamycin. Heterologous expressing VdFKBP12 in Arabidopsis conferred rapamycin sensitivity, indicating that VdFKBP12 can bridge the interaction between rapamycin and TOR across species. The key across species of TOR complex 1 (TORC1) and TORC2 have been identified in V. dahliae, suggesting that TOR signaling pathway is evolutionarily conserved in eukaryotic species. Furthermore, the RNA-seq analysis showed that ribosomal biogenesis, RNA polymerase II transcription factors and many metabolic processes were significantly suppressed in rapamycin treated cells of V. dahliae. Importantly, transcript levels of genes associated with cell wall degrading enzymes (CWEDs) were dramatically down-regulated in TOR-inhibited cells. Further infection assay showed that the pathogenicity of V. dahliae and occurrence of Verticillium wilt can be blocked in the presence of rapamycin. These observations suggested that VdTOR is a key target of V. dahliae for controlling and preventing Verticillium wilt in plants. |
| first_indexed | 2024-04-13T17:35:20Z |
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| id | doaj.art-b513da14fb6049dcb12114bfa1f0fe2d |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T17:35:20Z |
| publishDate | 2019-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-b513da14fb6049dcb12114bfa1f0fe2d2022-12-22T02:37:24ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-03-011010.3389/fmicb.2019.00501442562Functional Characterization of Target of Rapamycin Signaling in Verticillium dahliaeLinxuan Li0Tingting Zhu1Yun Song2Yun Song3Xiumei Luo4Li Feng5Fengping Zhuo6Fengping Zhuo7Fuguang Li8Fuguang Li9Maozhi Ren10School of Life Sciences, Chongqing University, Chongqing, ChinaSchool of Life Sciences, Chongqing University, Chongqing, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, ChinaNational Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, ChinaSchool of Life Sciences, Chongqing University, Chongqing, ChinaSchool of Life Sciences, Chongqing University, Chongqing, ChinaSchool of Life Sciences, Chongqing University, Chongqing, ChinaSchool of Chemistry and Chemical Engineering, Chongqing University of Science and Technology, Chongqing, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, ChinaNational Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, ChinaSchool of Life Sciences, Chongqing University, Chongqing, ChinaMore than 200 plants have been suffering from Verticillium wilt caused by Verticillium dahliae (V. dahliae) across the world. The target of rapamycin (TOR) is a lethal gene and controls cell growth and development in various eukaryotes, but little is known about TOR signaling in V. dahliae. Here, we found that V. dahliae strain is hypersensitive to rapamycin in the presence of rapamycin binding protein VdFKBP12 while the deletion mutant aaavdfkbp12 is insensitive to rapamycin. Heterologous expressing VdFKBP12 in Arabidopsis conferred rapamycin sensitivity, indicating that VdFKBP12 can bridge the interaction between rapamycin and TOR across species. The key across species of TOR complex 1 (TORC1) and TORC2 have been identified in V. dahliae, suggesting that TOR signaling pathway is evolutionarily conserved in eukaryotic species. Furthermore, the RNA-seq analysis showed that ribosomal biogenesis, RNA polymerase II transcription factors and many metabolic processes were significantly suppressed in rapamycin treated cells of V. dahliae. Importantly, transcript levels of genes associated with cell wall degrading enzymes (CWEDs) were dramatically down-regulated in TOR-inhibited cells. Further infection assay showed that the pathogenicity of V. dahliae and occurrence of Verticillium wilt can be blocked in the presence of rapamycin. These observations suggested that VdTOR is a key target of V. dahliae for controlling and preventing Verticillium wilt in plants.https://www.frontiersin.org/article/10.3389/fmicb.2019.00501/fullrapamycintarget of rapamycinpathogenicityVerticillium dahliaeVerticillium wilt |
| spellingShingle | Linxuan Li Tingting Zhu Yun Song Yun Song Xiumei Luo Li Feng Fengping Zhuo Fengping Zhuo Fuguang Li Fuguang Li Maozhi Ren Functional Characterization of Target of Rapamycin Signaling in Verticillium dahliae Frontiers in Microbiology rapamycin target of rapamycin pathogenicity Verticillium dahliae Verticillium wilt |
| title | Functional Characterization of Target of Rapamycin Signaling in Verticillium dahliae |
| title_full | Functional Characterization of Target of Rapamycin Signaling in Verticillium dahliae |
| title_fullStr | Functional Characterization of Target of Rapamycin Signaling in Verticillium dahliae |
| title_full_unstemmed | Functional Characterization of Target of Rapamycin Signaling in Verticillium dahliae |
| title_short | Functional Characterization of Target of Rapamycin Signaling in Verticillium dahliae |
| title_sort | functional characterization of target of rapamycin signaling in verticillium dahliae |
| topic | rapamycin target of rapamycin pathogenicity Verticillium dahliae Verticillium wilt |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.00501/full |
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