Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy
Abstract Porphyromonas gingivalis is one of the most important Gram-negative anaerobe bacteria involved in the pathogenesis of periodontitis. P. gingivalis has an arsenal of specialized virulence factors that contribute to its pathogenicity. Among them, fimbriae play a role in the initial attachment...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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University of São Paulo
2019-04-01
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| Series: | Journal of Applied Oral Science |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100441&lng=en&tlng=en |
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| author | Mariana Gouvêa Latini ABREU Dione KAWAMOTO Marcia Pinto Alves MAYER Vinicius D’Avila Bitencourt PASCOAL Karina Sampaio CAIAFFA Elizangela P. ZUZA Cristiane DUQUE Gabriela Alessandra da Cruz Galhardo CAMARGO |
| author_facet | Mariana Gouvêa Latini ABREU Dione KAWAMOTO Marcia Pinto Alves MAYER Vinicius D’Avila Bitencourt PASCOAL Karina Sampaio CAIAFFA Elizangela P. ZUZA Cristiane DUQUE Gabriela Alessandra da Cruz Galhardo CAMARGO |
| author_sort | Mariana Gouvêa Latini ABREU |
| collection | DOAJ |
| description | Abstract Porphyromonas gingivalis is one of the most important Gram-negative anaerobe bacteria involved in the pathogenesis of periodontitis. P. gingivalis has an arsenal of specialized virulence factors that contribute to its pathogenicity. Among them, fimbriae play a role in the initial attachment and organization of biofilms. Different genotypes of fimA have been related to length of fimbriae and pathogenicity of the bacterium. Objectives The aim of this study was to identify 5 types of fimA genotype strains in smokers and nonsmokers with periodontitis, before and after periodontal therapy. Material and Methods Thirty-one patients with periodontitis harboring P. gingivalis were selected: 16 nonsmokers (NS) and 15 smokers (SM). Clinical and microbiological parameters were evaluated at baseline and 3 months after periodontal treatment, namely: plaque index, bleeding on probe, probing depth, gingival recession and clinical attachment level. The frequency of P. gingivalis and fimA genotype strains were determined by polymerase chain reaction. Results Type I fimA was detected in the majority of SM and NS at baseline, and the frequency did not diminish after 3 months of treatment. The frequency of type II genotype was higher in SM than NS at baseline. After 3 months, statistical reduction was observed only for types II and V fimA genotypes in SM. The highest association was found between types I and II at baseline for NS (37.5%) and SM (53.3%). Conclusion The most prevalent P. gingivalis fimA genotypes detected in periodontal and smoker patients were genotypes I and II. However, the presence of fimA genotype II was higher in SM. Periodontal treatment was effective in controlling periodontal disease and reducing type II and V P. gingivalis fimA. |
| first_indexed | 2024-12-21T02:24:18Z |
| format | Article |
| id | doaj.art-b51677fac250449abb96cc63924f6522 |
| institution | Directory Open Access Journal |
| issn | 1678-7765 |
| language | English |
| last_indexed | 2024-12-21T02:24:18Z |
| publishDate | 2019-04-01 |
| publisher | University of São Paulo |
| record_format | Article |
| series | Journal of Applied Oral Science |
| spelling | doaj.art-b51677fac250449abb96cc63924f65222022-12-21T19:19:02ZengUniversity of São PauloJournal of Applied Oral Science1678-77652019-04-0127010.1590/1678-7757-2018-0205S1678-77572019000100441Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapyMariana Gouvêa Latini ABREUDione KAWAMOTOMarcia Pinto Alves MAYERVinicius D’Avila Bitencourt PASCOALKarina Sampaio CAIAFFAElizangela P. ZUZACristiane DUQUEGabriela Alessandra da Cruz Galhardo CAMARGOAbstract Porphyromonas gingivalis is one of the most important Gram-negative anaerobe bacteria involved in the pathogenesis of periodontitis. P. gingivalis has an arsenal of specialized virulence factors that contribute to its pathogenicity. Among them, fimbriae play a role in the initial attachment and organization of biofilms. Different genotypes of fimA have been related to length of fimbriae and pathogenicity of the bacterium. Objectives The aim of this study was to identify 5 types of fimA genotype strains in smokers and nonsmokers with periodontitis, before and after periodontal therapy. Material and Methods Thirty-one patients with periodontitis harboring P. gingivalis were selected: 16 nonsmokers (NS) and 15 smokers (SM). Clinical and microbiological parameters were evaluated at baseline and 3 months after periodontal treatment, namely: plaque index, bleeding on probe, probing depth, gingival recession and clinical attachment level. The frequency of P. gingivalis and fimA genotype strains were determined by polymerase chain reaction. Results Type I fimA was detected in the majority of SM and NS at baseline, and the frequency did not diminish after 3 months of treatment. The frequency of type II genotype was higher in SM than NS at baseline. After 3 months, statistical reduction was observed only for types II and V fimA genotypes in SM. The highest association was found between types I and II at baseline for NS (37.5%) and SM (53.3%). Conclusion The most prevalent P. gingivalis fimA genotypes detected in periodontal and smoker patients were genotypes I and II. However, the presence of fimA genotype II was higher in SM. Periodontal treatment was effective in controlling periodontal disease and reducing type II and V P. gingivalis fimA.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100441&lng=en&tlng=enPeriodontal diseaseSmokingPorphyromonas gingivalis |
| spellingShingle | Mariana Gouvêa Latini ABREU Dione KAWAMOTO Marcia Pinto Alves MAYER Vinicius D’Avila Bitencourt PASCOAL Karina Sampaio CAIAFFA Elizangela P. ZUZA Cristiane DUQUE Gabriela Alessandra da Cruz Galhardo CAMARGO Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy Journal of Applied Oral Science Periodontal disease Smoking Porphyromonas gingivalis |
| title | Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy |
| title_full | Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy |
| title_fullStr | Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy |
| title_full_unstemmed | Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy |
| title_short | Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy |
| title_sort | frequency of porphyromonas gingivalis fima in smokers and nonsmokers after periodontal therapy |
| topic | Periodontal disease Smoking Porphyromonas gingivalis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100441&lng=en&tlng=en |
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