Exploring Sensitive Label-Free Multiplex Analysis with Raman-Coded Microbeads and SERS-Coded Reporters
Suspension microsphere immunoassays are rapidly gaining attention in multiplex bioassays. Accurate detection of multiple analytes from a single measurement is critical in modern bioanalysis, which always requires complex encoding systems. In this study, a novel bioassay with Raman-coded antibody sup...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/2/121 |
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| author | Umar Azhar Qazi Ahmed Saira Ishaq Zeyad T. Alwahabi Sheng Dai |
| author_facet | Umar Azhar Qazi Ahmed Saira Ishaq Zeyad T. Alwahabi Sheng Dai |
| author_sort | Umar Azhar |
| collection | DOAJ |
| description | Suspension microsphere immunoassays are rapidly gaining attention in multiplex bioassays. Accurate detection of multiple analytes from a single measurement is critical in modern bioanalysis, which always requires complex encoding systems. In this study, a novel bioassay with Raman-coded antibody supports (polymer microbeads with different Raman signatures) and surface-enhanced Raman scattering (SERS)-coded nanotags (organic thiols on a gold nanoparticle surface with different SERS signatures) was developed as a model fluorescent, label-free, bead-based multiplex immunoassay system. The developed homogeneous immunoassays included two surface-functionalized monodisperse Raman-coded microbeads of polystyrene and poly(4-tert-butylstyrene) as the immune solid supports, and two epitope modified nanotags (self-assembled 4-mercaptobenzoic acid or 3-mercaptopropionic acid on gold nanoparticles) as the SERS-coded reporters. Such multiplex Raman/SERS-based microsphere immunoassays could selectively identify specific paratope–epitope interactions from one mixture sample solution under a single laser illumination, and thus hold great promise in future suspension multiplex analysis for diverse biomedical applications. |
| first_indexed | 2024-03-09T22:28:14Z |
| format | Article |
| id | doaj.art-b5181ef5b8d0400cbf4cd34703e60e36 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T22:28:14Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-b5181ef5b8d0400cbf4cd34703e60e362023-11-23T19:01:40ZengMDPI AGBiosensors2079-63742022-02-0112212110.3390/bios12020121Exploring Sensitive Label-Free Multiplex Analysis with Raman-Coded Microbeads and SERS-Coded ReportersUmar Azhar0Qazi Ahmed1Saira Ishaq2Zeyad T. Alwahabi3Sheng Dai4School of Chemical Engineering and Advanced Materials, The University of Adelaide, Adelaide, SA 5005, AustraliaSchool of Chemical Engineering and Advanced Materials, The University of Adelaide, Adelaide, SA 5005, AustraliaSchool of Chemical Engineering and Advanced Materials, The University of Adelaide, Adelaide, SA 5005, AustraliaSchool of Chemical Engineering and Advanced Materials, The University of Adelaide, Adelaide, SA 5005, AustraliaSchool of Chemical Engineering and Advanced Materials, The University of Adelaide, Adelaide, SA 5005, AustraliaSuspension microsphere immunoassays are rapidly gaining attention in multiplex bioassays. Accurate detection of multiple analytes from a single measurement is critical in modern bioanalysis, which always requires complex encoding systems. In this study, a novel bioassay with Raman-coded antibody supports (polymer microbeads with different Raman signatures) and surface-enhanced Raman scattering (SERS)-coded nanotags (organic thiols on a gold nanoparticle surface with different SERS signatures) was developed as a model fluorescent, label-free, bead-based multiplex immunoassay system. The developed homogeneous immunoassays included two surface-functionalized monodisperse Raman-coded microbeads of polystyrene and poly(4-tert-butylstyrene) as the immune solid supports, and two epitope modified nanotags (self-assembled 4-mercaptobenzoic acid or 3-mercaptopropionic acid on gold nanoparticles) as the SERS-coded reporters. Such multiplex Raman/SERS-based microsphere immunoassays could selectively identify specific paratope–epitope interactions from one mixture sample solution under a single laser illumination, and thus hold great promise in future suspension multiplex analysis for diverse biomedical applications.https://www.mdpi.com/2079-6374/12/2/121SERSRamanmicrobeadsmultipleximmunoassays |
| spellingShingle | Umar Azhar Qazi Ahmed Saira Ishaq Zeyad T. Alwahabi Sheng Dai Exploring Sensitive Label-Free Multiplex Analysis with Raman-Coded Microbeads and SERS-Coded Reporters Biosensors SERS Raman microbeads multiplex immunoassays |
| title | Exploring Sensitive Label-Free Multiplex Analysis with Raman-Coded Microbeads and SERS-Coded Reporters |
| title_full | Exploring Sensitive Label-Free Multiplex Analysis with Raman-Coded Microbeads and SERS-Coded Reporters |
| title_fullStr | Exploring Sensitive Label-Free Multiplex Analysis with Raman-Coded Microbeads and SERS-Coded Reporters |
| title_full_unstemmed | Exploring Sensitive Label-Free Multiplex Analysis with Raman-Coded Microbeads and SERS-Coded Reporters |
| title_short | Exploring Sensitive Label-Free Multiplex Analysis with Raman-Coded Microbeads and SERS-Coded Reporters |
| title_sort | exploring sensitive label free multiplex analysis with raman coded microbeads and sers coded reporters |
| topic | SERS Raman microbeads multiplex immunoassays |
| url | https://www.mdpi.com/2079-6374/12/2/121 |
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