The Roles of Two hfq Genes in the Virulence and Stress Resistance of Burkholderia glumae
The Hfq protein is a global small RNA chaperone that interacts with regulatory bacterial small RNAs (sRNA) and plays a role in the post-transcriptional regulation of gene expression. The roles of Hfq in the virulence and pathogenicity of several infectious bacteria have been reported. This study was...
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2018-10-01
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| Series: | The Plant Pathology Journal |
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| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6200039 |
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| author | Jieun Kim Mohamed Mannaa Namgyu Kim Chaeyeong Lee Juyun Kim Jungwook Park Hyun-Hee Lee Young-Su Seo |
| author_facet | Jieun Kim Mohamed Mannaa Namgyu Kim Chaeyeong Lee Juyun Kim Jungwook Park Hyun-Hee Lee Young-Su Seo |
| author_sort | Jieun Kim |
| collection | DOAJ |
| description | The Hfq protein is a global small RNA chaperone that interacts with regulatory bacterial small RNAs (sRNA) and plays a role in the post-transcriptional regulation of gene expression. The roles of Hfq in the virulence and pathogenicity of several infectious bacteria have been reported. This study was conducted to elucidate the functions of two hfq genes in Burkholderia glumae, a causal agent of rice grain rot. Therefore, mutant strains of the rice-pathogenic B. glumae BGR1, targeting each of the two hfq genes, as well as the double defective mutant were constructed and tested for several phenotypic characteristics. Bacterial swarming motility, toxoflavin production, virulence in rice, siderophore production, sensitivity to H₂O₂, and lipase production assays were conducted to compare the mutant strains with the wild-type B. glumae BGR1 and complementation strains. The hfq1 gene showed more influence on bacterial motility and toxoflavin production than the hfq2 gene. Both genes were involved in the full virulence of B. glumae in rice plants. Other biochemical characteristics such as siderophore production and sensitivity to H₂O₂ induced oxidative stress were also found to be regulated by the hfq1 gene. However, lipase activity was shown to be unassociated with both tested genes. To the best of our knowledge, this is the first study to elucidate the functions of two hfq genes in B. glumae. Identification of virulence-related factors in B. glumae will facilitate the development of efficient control measures. |
| first_indexed | 2024-12-23T20:33:26Z |
| format | Article |
| id | doaj.art-b518ec224adb4f0ca08c73d24cc0780d |
| institution | Directory Open Access Journal |
| issn | 1598-2254 |
| language | English |
| last_indexed | 2024-12-23T20:33:26Z |
| publishDate | 2018-10-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-b518ec224adb4f0ca08c73d24cc0780d2022-12-21T17:32:10ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542018-10-0134541242510.5423/PPJ.OA.06.2018.009710.5423PPJ.OA.06.2018.0097The Roles of Two hfq Genes in the Virulence and Stress Resistance of Burkholderia glumaeJieun Kim0Mohamed Mannaa1Namgyu Kim2Chaeyeong Lee3Juyun Kim4Jungwook Park5Hyun-Hee Lee6Young-Su Seo7Department of Microbiology, Pusan National University, Busan 46241, KoreaDepartment of Microbiology, Pusan National University, Busan 46241, KoreaDepartment of Microbiology, Pusan National University, Busan 46241, KoreaDepartment of Microbiology, Pusan National University, Busan 46241, KoreaDepartment of Microbiology, Pusan National University, Busan 46241, KoreaDepartment of Microbiology, Pusan National University, Busan 46241, KoreaDepartment of Microbiology, Pusan National University, Busan 46241, KoreaDepartment of Microbiology, Pusan National University, Busan 46241, KoreaThe Hfq protein is a global small RNA chaperone that interacts with regulatory bacterial small RNAs (sRNA) and plays a role in the post-transcriptional regulation of gene expression. The roles of Hfq in the virulence and pathogenicity of several infectious bacteria have been reported. This study was conducted to elucidate the functions of two hfq genes in Burkholderia glumae, a causal agent of rice grain rot. Therefore, mutant strains of the rice-pathogenic B. glumae BGR1, targeting each of the two hfq genes, as well as the double defective mutant were constructed and tested for several phenotypic characteristics. Bacterial swarming motility, toxoflavin production, virulence in rice, siderophore production, sensitivity to H₂O₂, and lipase production assays were conducted to compare the mutant strains with the wild-type B. glumae BGR1 and complementation strains. The hfq1 gene showed more influence on bacterial motility and toxoflavin production than the hfq2 gene. Both genes were involved in the full virulence of B. glumae in rice plants. Other biochemical characteristics such as siderophore production and sensitivity to H₂O₂ induced oxidative stress were also found to be regulated by the hfq1 gene. However, lipase activity was shown to be unassociated with both tested genes. To the best of our knowledge, this is the first study to elucidate the functions of two hfq genes in B. glumae. Identification of virulence-related factors in B. glumae will facilitate the development of efficient control measures.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6200039Burkholderia glumaegene functionshfq geneRNA chaperone |
| spellingShingle | Jieun Kim Mohamed Mannaa Namgyu Kim Chaeyeong Lee Juyun Kim Jungwook Park Hyun-Hee Lee Young-Su Seo The Roles of Two hfq Genes in the Virulence and Stress Resistance of Burkholderia glumae The Plant Pathology Journal Burkholderia glumae gene functions hfq gene RNA chaperone |
| title | The Roles of Two hfq Genes in the Virulence and Stress Resistance of Burkholderia glumae |
| title_full | The Roles of Two hfq Genes in the Virulence and Stress Resistance of Burkholderia glumae |
| title_fullStr | The Roles of Two hfq Genes in the Virulence and Stress Resistance of Burkholderia glumae |
| title_full_unstemmed | The Roles of Two hfq Genes in the Virulence and Stress Resistance of Burkholderia glumae |
| title_short | The Roles of Two hfq Genes in the Virulence and Stress Resistance of Burkholderia glumae |
| title_sort | roles of two hfq genes in the virulence and stress resistance of burkholderia glumae |
| topic | Burkholderia glumae gene functions hfq gene RNA chaperone |
| url | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6200039 |
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