Analysis of the Properties of 44 ABC Transporter Genes from Biocontrol Agent <i>Trichoderma asperellum</i> ACCC30536 and Their Responses to Pathogenic <i>Alternaria alternata</i> Toxin Stress
ATP-binding cassette (ABC) transporters are involved in transporting multiple substrates, such as toxins, and may be important for the survival of <i>Trichoderma</i> when encountering biotic toxins. In this study, genome searching revealed that there are 44 ABC transporters encoded in th...
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| Langue: | English |
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MDPI AG
2023-02-01
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| Collection: | Current Issues in Molecular Biology |
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| Accès en ligne: | https://www.mdpi.com/1467-3045/45/2/101 |
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| author | Hua-Ying Du Yu-Zhou Zhang Kuo Liu Pei-Wen Gu Shuang Cao Xiang Gao Zhi-Ying Wang Zhi-Hua Liu Ze-Yang Yu |
| author_facet | Hua-Ying Du Yu-Zhou Zhang Kuo Liu Pei-Wen Gu Shuang Cao Xiang Gao Zhi-Ying Wang Zhi-Hua Liu Ze-Yang Yu |
| author_sort | Hua-Ying Du |
| collection | DOAJ |
| description | ATP-binding cassette (ABC) transporters are involved in transporting multiple substrates, such as toxins, and may be important for the survival of <i>Trichoderma</i> when encountering biotic toxins. In this study, genome searching revealed that there are 44 ABC transporters encoded in the genome of <i>Trichoderma asperellum</i>. These ABC transporters were divided into six types based on three-dimensional (3D) structure prediction, of which four, represented by 39 ABCs, are involved in transport and the remaining two, represented by 5 ABCs, are involved in regulating translation. The characteristics of nucleotide-binding domain (NBD) are important in the identification of ABC proteins. Even though the 3D structures of the 79 NBDs in the 44 ABCs are similar, multiple sequence alignment showed they can be divided into three classes. In total, 794 motifs were found in the promoter regions of the 44 <i>ABC</i> genes, of which 541 were cis-regulators related to stress responses. To characterize how their ABCs respond when <i>T. asperellum</i> interact with fungi or plants, <i>T. asperellum</i> was cultivated in either minimal media (MM) control, C-hungry, N-hungry, or poplar medium (PdPap) to simulate normal conditions, competition with pathogens, interaction with pathogens, and interaction with plants, respectively. The results show that 17 of 39 transport ABCs are highly expressed in at least one condition, whereas four of the five translation-regulating ABCs are highly expressed in at least one condition. Of these 21 highly expressed <i>ABCs</i>, 6 were chosen for RT-qPCR expression under the toxin stress of phytopathogen <i>Alternaria alternata</i>, and the results show <i>ABC01</i>, <i>ABC04</i>, <i>ABC05</i>, and <i>ABC31</i> were highly expressed and may be involved in pathogen interaction and detoxifying toxins from <i>A. alternata</i>. |
| first_indexed | 2024-03-11T08:59:16Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-11T08:59:16Z |
| publishDate | 2023-02-01 |
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| series | Current Issues in Molecular Biology |
| spelling | doaj.art-b51a7900a2884ef199f4db5b8352dd402023-11-16T19:51:08ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-02-014521570158610.3390/cimb45020101Analysis of the Properties of 44 ABC Transporter Genes from Biocontrol Agent <i>Trichoderma asperellum</i> ACCC30536 and Their Responses to Pathogenic <i>Alternaria alternata</i> Toxin StressHua-Ying Du0Yu-Zhou Zhang1Kuo Liu2Pei-Wen Gu3Shuang Cao4Xiang Gao5Zhi-Ying Wang6Zhi-Hua Liu7Ze-Yang Yu8School of Agriculture, Ningxia University, 489 Helan Mountain West Road, Yinchuan 750021, ChinaSchool of Forestry, Northeast Forestry University, 26 Hexing Road, Harbin 150040, ChinaSchool of Agriculture, Ningxia University, 489 Helan Mountain West Road, Yinchuan 750021, ChinaSchool of Agriculture, Ningxia University, 489 Helan Mountain West Road, Yinchuan 750021, ChinaSchool of Agriculture, Ningxia University, 489 Helan Mountain West Road, Yinchuan 750021, ChinaSchool of Agriculture, Ningxia University, 489 Helan Mountain West Road, Yinchuan 750021, ChinaSchool of Forestry, Northeast Forestry University, 26 Hexing Road, Harbin 150040, ChinaSchool of Forestry, Northeast Forestry University, 26 Hexing Road, Harbin 150040, ChinaSchool of Agriculture, Ningxia University, 489 Helan Mountain West Road, Yinchuan 750021, ChinaATP-binding cassette (ABC) transporters are involved in transporting multiple substrates, such as toxins, and may be important for the survival of <i>Trichoderma</i> when encountering biotic toxins. In this study, genome searching revealed that there are 44 ABC transporters encoded in the genome of <i>Trichoderma asperellum</i>. These ABC transporters were divided into six types based on three-dimensional (3D) structure prediction, of which four, represented by 39 ABCs, are involved in transport and the remaining two, represented by 5 ABCs, are involved in regulating translation. The characteristics of nucleotide-binding domain (NBD) are important in the identification of ABC proteins. Even though the 3D structures of the 79 NBDs in the 44 ABCs are similar, multiple sequence alignment showed they can be divided into three classes. In total, 794 motifs were found in the promoter regions of the 44 <i>ABC</i> genes, of which 541 were cis-regulators related to stress responses. To characterize how their ABCs respond when <i>T. asperellum</i> interact with fungi or plants, <i>T. asperellum</i> was cultivated in either minimal media (MM) control, C-hungry, N-hungry, or poplar medium (PdPap) to simulate normal conditions, competition with pathogens, interaction with pathogens, and interaction with plants, respectively. The results show that 17 of 39 transport ABCs are highly expressed in at least one condition, whereas four of the five translation-regulating ABCs are highly expressed in at least one condition. Of these 21 highly expressed <i>ABCs</i>, 6 were chosen for RT-qPCR expression under the toxin stress of phytopathogen <i>Alternaria alternata</i>, and the results show <i>ABC01</i>, <i>ABC04</i>, <i>ABC05</i>, and <i>ABC31</i> were highly expressed and may be involved in pathogen interaction and detoxifying toxins from <i>A. alternata</i>.https://www.mdpi.com/1467-3045/45/2/101biological controlABC proteindetoxification |
| spellingShingle | Hua-Ying Du Yu-Zhou Zhang Kuo Liu Pei-Wen Gu Shuang Cao Xiang Gao Zhi-Ying Wang Zhi-Hua Liu Ze-Yang Yu Analysis of the Properties of 44 ABC Transporter Genes from Biocontrol Agent <i>Trichoderma asperellum</i> ACCC30536 and Their Responses to Pathogenic <i>Alternaria alternata</i> Toxin Stress Current Issues in Molecular Biology biological control ABC protein detoxification |
| title | Analysis of the Properties of 44 ABC Transporter Genes from Biocontrol Agent <i>Trichoderma asperellum</i> ACCC30536 and Their Responses to Pathogenic <i>Alternaria alternata</i> Toxin Stress |
| title_full | Analysis of the Properties of 44 ABC Transporter Genes from Biocontrol Agent <i>Trichoderma asperellum</i> ACCC30536 and Their Responses to Pathogenic <i>Alternaria alternata</i> Toxin Stress |
| title_fullStr | Analysis of the Properties of 44 ABC Transporter Genes from Biocontrol Agent <i>Trichoderma asperellum</i> ACCC30536 and Their Responses to Pathogenic <i>Alternaria alternata</i> Toxin Stress |
| title_full_unstemmed | Analysis of the Properties of 44 ABC Transporter Genes from Biocontrol Agent <i>Trichoderma asperellum</i> ACCC30536 and Their Responses to Pathogenic <i>Alternaria alternata</i> Toxin Stress |
| title_short | Analysis of the Properties of 44 ABC Transporter Genes from Biocontrol Agent <i>Trichoderma asperellum</i> ACCC30536 and Their Responses to Pathogenic <i>Alternaria alternata</i> Toxin Stress |
| title_sort | analysis of the properties of 44 abc transporter genes from biocontrol agent i trichoderma asperellum i accc30536 and their responses to pathogenic i alternaria alternata i toxin stress |
| topic | biological control ABC protein detoxification |
| url | https://www.mdpi.com/1467-3045/45/2/101 |
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