Osteogenic differentiation of adipose tissue-derived mesenchymal stem cells cultured with different concentrations of prolactin
ABSTRACT The objective was to evaluate the in vitro effect of prolactin in osteogenic potential of adipose tissue-derived mesenchymal stem cells (ADSCs) in female rats. ADSCs were cultured in osteogenic medium with and without the addition of prolactin and distributed into three groups: 1) ADSCs (co...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Minas Gerais
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| Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000601573&lng=en&tlng=en |
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| author | K.P. Oliveira A.M.S. Reis A.P. Silva C.L.R. Silva A.M. Goes R. Serakides N.M. Ocarino |
| author_facet | K.P. Oliveira A.M.S. Reis A.P. Silva C.L.R. Silva A.M. Goes R. Serakides N.M. Ocarino |
| author_sort | K.P. Oliveira |
| collection | DOAJ |
| description | ABSTRACT The objective was to evaluate the in vitro effect of prolactin in osteogenic potential of adipose tissue-derived mesenchymal stem cells (ADSCs) in female rats. ADSCs were cultured in osteogenic medium with and without the addition of prolactin and distributed into three groups: 1) ADSCs (control), 2) ADSCs with addition of 100ng/mL of prolactin and 3) ADSCs with addition of 300ng/mL of prolactin. At 21 days of differentiation, the tests of MTT conversion into formazan crystals, percentage of mineralized nodules and cells per field and quantification of genic transcript for alkaline phosphatase, osteopontin, osteocalcin, bone sialoprotein, BMP-2 and collagen I by real-time RT-PCR were made. The addition of prolactin reduced the conversion of MTT in group 3 and increased the percentage of cells per field in the groups 2 and 3, however without significantly increasing the percentage of mineralized nodules and the expression of alkaline phosphatase, osteopontin, osteocalcin, bone sialoprotein, BMP-2 and collagen I. In conclusion, the addition of prolactin in concentrations of 100ng/mL and 300ng/mL does not change the osteogenic differentiation to the ADSCs of female rats despite increase in the cellularity of the culture. |
| first_indexed | 2024-12-14T11:11:56Z |
| format | Article |
| id | doaj.art-b51cba214d76434fa241ff6c1c7feb73 |
| institution | Directory Open Access Journal |
| issn | 1678-4162 |
| language | English |
| last_indexed | 2024-12-14T11:11:56Z |
| publisher | Universidade Federal de Minas Gerais |
| record_format | Article |
| series | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| spelling | doaj.art-b51cba214d76434fa241ff6c1c7feb732022-12-21T23:04:14ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-41626961573158010.1590/1678-4162-9364S0102-09352017000601573Osteogenic differentiation of adipose tissue-derived mesenchymal stem cells cultured with different concentrations of prolactinK.P. OliveiraA.M.S. ReisA.P. SilvaC.L.R. SilvaA.M. GoesR. SerakidesN.M. OcarinoABSTRACT The objective was to evaluate the in vitro effect of prolactin in osteogenic potential of adipose tissue-derived mesenchymal stem cells (ADSCs) in female rats. ADSCs were cultured in osteogenic medium with and without the addition of prolactin and distributed into three groups: 1) ADSCs (control), 2) ADSCs with addition of 100ng/mL of prolactin and 3) ADSCs with addition of 300ng/mL of prolactin. At 21 days of differentiation, the tests of MTT conversion into formazan crystals, percentage of mineralized nodules and cells per field and quantification of genic transcript for alkaline phosphatase, osteopontin, osteocalcin, bone sialoprotein, BMP-2 and collagen I by real-time RT-PCR were made. The addition of prolactin reduced the conversion of MTT in group 3 and increased the percentage of cells per field in the groups 2 and 3, however without significantly increasing the percentage of mineralized nodules and the expression of alkaline phosphatase, osteopontin, osteocalcin, bone sialoprotein, BMP-2 and collagen I. In conclusion, the addition of prolactin in concentrations of 100ng/mL and 300ng/mL does not change the osteogenic differentiation to the ADSCs of female rats despite increase in the cellularity of the culture.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000601573&lng=en&tlng=enratosteogenic differentiationosteoblastshormoneadipose tissue |
| spellingShingle | K.P. Oliveira A.M.S. Reis A.P. Silva C.L.R. Silva A.M. Goes R. Serakides N.M. Ocarino Osteogenic differentiation of adipose tissue-derived mesenchymal stem cells cultured with different concentrations of prolactin Arquivo Brasileiro de Medicina Veterinária e Zootecnia rat osteogenic differentiation osteoblasts hormone adipose tissue |
| title | Osteogenic differentiation of adipose tissue-derived mesenchymal stem cells cultured with different concentrations of prolactin |
| title_full | Osteogenic differentiation of adipose tissue-derived mesenchymal stem cells cultured with different concentrations of prolactin |
| title_fullStr | Osteogenic differentiation of adipose tissue-derived mesenchymal stem cells cultured with different concentrations of prolactin |
| title_full_unstemmed | Osteogenic differentiation of adipose tissue-derived mesenchymal stem cells cultured with different concentrations of prolactin |
| title_short | Osteogenic differentiation of adipose tissue-derived mesenchymal stem cells cultured with different concentrations of prolactin |
| title_sort | osteogenic differentiation of adipose tissue derived mesenchymal stem cells cultured with different concentrations of prolactin |
| topic | rat osteogenic differentiation osteoblasts hormone adipose tissue |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000601573&lng=en&tlng=en |
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