Diversity of Filamentous Fungi Associated with Dairy Processing Environments and Spoiled Products in Brazil
Few studies have investigated the diversity of spoilage fungi from the dairy production chain in Brazil, despite their importance as spoilage microorganisms. In the present study, 109 filamentous fungi were isolated from various spoiled dairy products and dairy production environments. The isolates...
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| 格式: | 文件 |
| 语言: | English |
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MDPI AG
2022-12-01
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| 丛编: | Foods |
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| 在线阅读: | https://www.mdpi.com/2304-8158/12/1/153 |
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| author | Luana Virgínia Souza Rafaela da Silva Rodrigues Andressa Fusieger Raiane Rodrigues da Silva Sidney Rodrigues de Jesus Silva Evandro Martins Solimar Gonçalves Machado Cinzia Caggia Cinzia Lucia Randazzo Antonio Fernandes de Carvalho |
| author_facet | Luana Virgínia Souza Rafaela da Silva Rodrigues Andressa Fusieger Raiane Rodrigues da Silva Sidney Rodrigues de Jesus Silva Evandro Martins Solimar Gonçalves Machado Cinzia Caggia Cinzia Lucia Randazzo Antonio Fernandes de Carvalho |
| author_sort | Luana Virgínia Souza |
| collection | DOAJ |
| description | Few studies have investigated the diversity of spoilage fungi from the dairy production chain in Brazil, despite their importance as spoilage microorganisms. In the present study, 109 filamentous fungi were isolated from various spoiled dairy products and dairy production environments. The isolates were identified through sequencing of the internal transcribed spacer (ITS) region. In spoiled products, <i>Penicillium</i> and <i>Cladosporium</i> were the most frequent genera of filamentous fungi and were also present in the dairy environment, indicating that they may represent a primary source of contamination. For dairy production environments, the most frequent genera were <i>Cladosporium</i>, <i>Penicillium</i>, <i>Aspergillus</i>, and <i>Nigrospora</i>. Four species (<i>Hypoxylon griseobrunneum</i>, <i>Rhinocladiella similis</i>, <i>Coniochaeta rosae</i>, and <i>Paecilomyces maximus</i>) were identified for the first time in dairy products or in dairy production environment. Phytopathogenic genera were also detected, such as <i>Montagnula</i>, <i>Clonostachys</i>, and <i>Riopa</i>. One species isolated from the dairy production environment is classified as the pathogenic fungi, <i>R. similis</i>. Regarding the phylogeny, 14 different families were observed and most of the fungi belong to the Ascomycota phylum. The understanding of fungal biodiversity in dairy products and environment can support the development of conservation strategies to control food spoilage. This includes the suitable use of preservatives in dairy products, as well as the application of specific cleaning and sanitizing protocols designed for a specific group of target microorganisms. |
| first_indexed | 2024-03-11T10:01:08Z |
| format | Article |
| id | doaj.art-b51cdf81228a44f6ac62c737524c157d |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T10:01:08Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-b51cdf81228a44f6ac62c737524c157d2023-11-16T15:23:07ZengMDPI AGFoods2304-81582022-12-0112115310.3390/foods12010153Diversity of Filamentous Fungi Associated with Dairy Processing Environments and Spoiled Products in BrazilLuana Virgínia Souza0Rafaela da Silva Rodrigues1Andressa Fusieger2Raiane Rodrigues da Silva3Sidney Rodrigues de Jesus Silva4Evandro Martins5Solimar Gonçalves Machado6Cinzia Caggia7Cinzia Lucia Randazzo8Antonio Fernandes de Carvalho9InovaLeite—Laboratório de Pesquisa em Leites e Derivados, Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Viçosa 36570-900, MG, BrazilInovaLeite—Laboratório de Pesquisa em Leites e Derivados, Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Viçosa 36570-900, MG, BrazilInovaLeite—Laboratório de Pesquisa em Leites e Derivados, Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Viçosa 36570-900, MG, BrazilInovaLeite—Laboratório de Pesquisa em Leites e Derivados, Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Viçosa 36570-900, MG, BrazilInovaLeite—Laboratório de Pesquisa em Leites e Derivados, Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Viçosa 36570-900, MG, BrazilInovaLeite—Laboratório de Pesquisa em Leites e Derivados, Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Viçosa 36570-900, MG, BrazilInovaLeite—Laboratório de Pesquisa em Leites e Derivados, Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Viçosa 36570-900, MG, BrazilDi3A—Dipartimento di Agricoltura, Alimentazione e Ambiente, Università degli Studi di Catania, 95123 Catania, ItalyDi3A—Dipartimento di Agricoltura, Alimentazione e Ambiente, Università degli Studi di Catania, 95123 Catania, ItalyInovaLeite—Laboratório de Pesquisa em Leites e Derivados, Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Viçosa 36570-900, MG, BrazilFew studies have investigated the diversity of spoilage fungi from the dairy production chain in Brazil, despite their importance as spoilage microorganisms. In the present study, 109 filamentous fungi were isolated from various spoiled dairy products and dairy production environments. The isolates were identified through sequencing of the internal transcribed spacer (ITS) region. In spoiled products, <i>Penicillium</i> and <i>Cladosporium</i> were the most frequent genera of filamentous fungi and were also present in the dairy environment, indicating that they may represent a primary source of contamination. For dairy production environments, the most frequent genera were <i>Cladosporium</i>, <i>Penicillium</i>, <i>Aspergillus</i>, and <i>Nigrospora</i>. Four species (<i>Hypoxylon griseobrunneum</i>, <i>Rhinocladiella similis</i>, <i>Coniochaeta rosae</i>, and <i>Paecilomyces maximus</i>) were identified for the first time in dairy products or in dairy production environment. Phytopathogenic genera were also detected, such as <i>Montagnula</i>, <i>Clonostachys</i>, and <i>Riopa</i>. One species isolated from the dairy production environment is classified as the pathogenic fungi, <i>R. similis</i>. Regarding the phylogeny, 14 different families were observed and most of the fungi belong to the Ascomycota phylum. The understanding of fungal biodiversity in dairy products and environment can support the development of conservation strategies to control food spoilage. This includes the suitable use of preservatives in dairy products, as well as the application of specific cleaning and sanitizing protocols designed for a specific group of target microorganisms.https://www.mdpi.com/2304-8158/12/1/153air factory contamination<i>Cladosporium</i>dairy chainfood safetyspoilage fungi |
| spellingShingle | Luana Virgínia Souza Rafaela da Silva Rodrigues Andressa Fusieger Raiane Rodrigues da Silva Sidney Rodrigues de Jesus Silva Evandro Martins Solimar Gonçalves Machado Cinzia Caggia Cinzia Lucia Randazzo Antonio Fernandes de Carvalho Diversity of Filamentous Fungi Associated with Dairy Processing Environments and Spoiled Products in Brazil Foods air factory contamination <i>Cladosporium</i> dairy chain food safety spoilage fungi |
| title | Diversity of Filamentous Fungi Associated with Dairy Processing Environments and Spoiled Products in Brazil |
| title_full | Diversity of Filamentous Fungi Associated with Dairy Processing Environments and Spoiled Products in Brazil |
| title_fullStr | Diversity of Filamentous Fungi Associated with Dairy Processing Environments and Spoiled Products in Brazil |
| title_full_unstemmed | Diversity of Filamentous Fungi Associated with Dairy Processing Environments and Spoiled Products in Brazil |
| title_short | Diversity of Filamentous Fungi Associated with Dairy Processing Environments and Spoiled Products in Brazil |
| title_sort | diversity of filamentous fungi associated with dairy processing environments and spoiled products in brazil |
| topic | air factory contamination <i>Cladosporium</i> dairy chain food safety spoilage fungi |
| url | https://www.mdpi.com/2304-8158/12/1/153 |
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